Analysis of the mechanism that elaborate an alternative pathogenicity by loss of capsule in Streptococcus suis

• Project/Area Number: 23580420
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: The University of Tokyo
• Principal Investigator: SEKIZAKI TSUTOMU 東京大学, 農学生命科学研究科, 教授 (70355163)
• Co-Investigator(Renkei-kenkyūsha): OSAWA Ro 神戸大学, 大学院農学研究科, 教授 (10253189) ; OSAKI Makoto 独立行政法人農業・食品産業技術総合研究 機構, 動物衛生研究所, 主任研究員 (80355164) ; TAKAMATSU Daisuke 独立行政法人農業・食品産業技術総合研究 機構, 動物衛生研究所, 主任研究員 (60414728) ; OKURA Masatoshi 独立行政法人農業・食品産業技術総合研究 機構, 動物衛生研究所, 研究員 (60508315)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 人獣共通感染症 / 獣医細菌学 / 分子遺伝学 / レンサ球菌 / 病原因子 / ブタ / 莢膜 / 豚レンサ球菌 / 心内膜炎 / 遺伝子変異

Research Abstract

Streptococcus suis is a bacterial pathogen that causes meningitis or endocarditis in pigs and humans. Capsule, a surface component produced by this bacterium, is an important virulence factor that inhibits phagocytosis of white blood cells. This study showed that acapsular cells of S. suis, which have sometimes been found in swine endocarditis isolates, have spontaneous mutations in capsular polysaccharide synthesis (cps) genes, and that mutations in some cps genes appeared lethal for the bacteria, whereas these lethal effects were relieved by mutations in the other cps genes. Moreover, acapsular cells showed elevated adherence to porcine and human platelets, a major virulence determinant for infective endocarditis, compared with capsular cells and the acapsular cells facilitated the incorporation of capsular cells to the biofilm formed by acapsular cells.

Research Products

• [Journal Article] Streptococcus suis serotyping by a new multiplex PCR (2014)
  • Author(s): Kerdsin A, Akeda Y, Hatrongjit R, Detchawna U, Sekizaki T, Hamada S, Gottschalk M, Oishi K.
  • Journal Title: J Med Microbiol Volume: 63 Pages: 824-830
• [Journal Article] Capsule loss or death : The position of mutations among capsule genes sways the destiny of Streptococcus suis (2014)
  • Author(s): Lakkitjaroen, N., Takamatsu, D., Okura, M., Sato, M., Osaki, M., and Sekizaki, T.
  • Journal Title: FEMS Microbiol. Lett Volume: 354 Pages: 46-54
• [Journal Article] Development of a two-step multiplex PCR assay for typing of capsular polysaccharide synthesis gene clusters of Streptococcus suis (2014)
  • Author(s): Okura, M., Lachance, C., Osaki, M., Sekizaki, T., Maruyama, F., Nozawa, T., Nakagawa, I., Hamada, S., Rossignol, C., Gottschalk, M. and Takamatsu, D.
  • Journal Title: J. Clin. Microbiol Volume: 52 Pages: 1714-1719
• [Journal Article] Development of a two-step multiplex PCR assay for typing of capsular polysaccharide synthesis gene clusters of Streptococcus suis (2014)
  • Author(s): Okura M, Lachance C, Osaki M, Sekizaki T, Maruyama F, Nozawa T, Nakagawa I, Hamada S, Rossignol C, Gottschalk M, Takamatsu D
  • Journal Title: J Clin Microbiol Volume: 52 Issue: 5 Pages: 1714-1719
  • DOI: 10.1128/jcm.03411-13
  • Peer Reviewed / Open Access
• [Journal Article] Capsule loss or death: The position of mutations among capsule genes sways the destiny of Streptococcus suis. (2014)
  • Author(s): Lakkitjaroen, N., Takamatsu, D., Okura, M., Sato, M., Osaki, M., and Sekizaki, T.
  • Journal Title: FEMS Microbiol. Lett. Volume: 353 Issue: 1 Pages: 46-54
  • DOI: 10.1111/1574-6968.12428
  • Peer Reviewed
• [Journal Article] Streptococcus suis Serotyping by a New Multiplex PCR. (2014)
  • Author(s): Kerdsin A, Akeda Y, Hatrongjit R, Detchawna U, Sekizaki T, Hamada S, Gottschalk M, Oishi K.
  • Journal Title: J Med Microbiol. Volume: 63 Issue: 6 Pages: 824-830
  • DOI: 10.1099/jmm.0.069757-0
  • Peer Reviewed
• [Journal Article] Antimicrobial susceptibility of Streptococcus gallolyticus isolated from humans and animals (2013)
  • Author(s): Nomoto R., Tien le H.T., Sekizaki T., and Osawa R.
  • Journal Title: Jpn J Infect Dis Volume: 66(4) Pages: 334-336
  • NAID: 130003381700
• [Journal Article] Genetic analysis of capsular polysaccharide synthesis 1 gene clusters from all serotypes of Streptococcus suis : potential mechanisms for the generation of capsular variation (2013)
  • Author(s): Okura, M., Takamatsu, D., Maruyama, F., Nozawa, T., Nakagawa, I., Osaki, M., Sekizaki, T., Gottschalk, M., Kumagai, Y., and Hamada, S.
  • Journal Title: Appl. Environ. Microbiol Volume: 79 Pages: 2796-2806
• [Journal Article] Antimicrobial susceptibility of Streptococcus gallolyticus isolated from humans and animals. (2013)
  • Author(s): Nomoto R., Tien le H.T., Sekizaki T., and Osawa R.
  • Journal Title: Jpn J Infect Dis. Volume: 66 Pages: 334-336
  • NAID: 130003381700
  • Peer Reviewed
• [Journal Article] Consumer awareness and attitude on radiocesium food contamination following Fukushima incident. (2013)
  • Author(s): Hosono, H., Kumagai, Y., and Sekizaki, T.
  • Journal Title: J. Disaster Res. Volume: 8 Pages: 762-772
  • Peer Reviewed
• [Journal Article] Genetic analysis of capsular polysaccharide synthesis gene clusters from all serotypes of Streptococcus suis : potential mechanisms for generation of capsular variation (2013)
  • Author(s): Okura M, Takamatsu D, Maruyama F, Nozawa T, Nakagawa I, Osaki M, Sekizaki T, Gottschalk M, Kumagai Y, Hamada S
  • Journal Title: Appl Environ Microbiol Volume: 79 Issue: 8 Pages: 2796-2806
  • DOI: 10.1128/aem.03742-12
  • Peer Reviewed
• [Journal Article] Fifteen Streptococcus suis serotypes identified by multiplex PCR (2012)
  • Author(s): Anusak, K., Dejsirilert, S., Akeda, Y., Sekizaki, T., Hamada, S., Gottschalk, M., Oishi, K.
  • Journal Title: J. Med. Microbiol Volume: 61 Pages: 1669-1672
• [Journal Article] Streptococcus suis : an emerging biothreat (2012)
  • Author(s): Sekizaki, T.
  • Journal Title: Journal of Disaster Research Volume: 7 (3) Pages: 303-312
• [Journal Article] Fifteen Streptococcus suis serotypes identified by multiplex PCR. (2012)
  • Author(s): Kerdsin A
  • Journal Title: Journal of Medical Microbiology Volume: 61 Issue: 12 Pages: 1669-1672
  • DOI: 10.1099/jmm.0.048587-0
  • Peer Reviewed
• [Journal Article] Streptococcus suis: an emerging biothreat (2012)
  • Author(s): Sekizaki, T.
  • Journal Title: J. Disaster Res. Volume: 7 Pages: 303-312
  • URL: http://www.fujipress.jp/finder/xslt.php?mode=present&inputfile=DSSTR000700030008.xml
  • Peer Reviewed
• [Journal Article] Lineage and virulence of Streptococcus suis serotype 2 isolates from North America (2011)
  • Author(s): Fittipaldi, N., Xu, J., Lacouture, S., Tharavichitkul, P., Osaki, M., Sekizaki, T., Takamatsu, D., Gottschalk, M.
  • Journal Title: Emerg. Infect. Dis Volume: 17 Pages: 2239-44
• [Journal Article] Loss of capsule among Streptococcus suis isolates from porcine endocarditis and its biological significance (2011)
  • Author(s): Lakkitjaroen, N., Takamatsu, D., Okura, M., Sato, M., Osaki, M., Sekizaki, T.
  • Journal Title: J. Med. Microbiol Volume: 60 Pages: 1669-1676
• [Journal Article] The minor pilin subunit Sgp2 is necessary for assembly of the pilus encoded by the srtG cluster of Streptococcus suis (2011)
  • Author(s): Okura, M., Osaki, M., Fittipaldi, N., Gottschalk, M., Sekizaki, T., and Takamatsu, D.
  • Journal Title: J. Bacteriol Volume: 193 Pages: 822-831
• [Journal Article] Loss of capsule among Streptococcus suis isolates from porcine endocarditis and its biological significance. (2011)
  • Author(s): Lakkitjaroen, N., Takamatsu, D., Okura, M., Sato, M., Osaki, M., Sekizaki, T.
  • Journal Title: J. Med. Microbiol. Volume: 60 Issue: 11 Pages: 1669-1676
  • DOI: 10.1099/jmm.0.034686-0
  • Peer Reviewed
• [Journal Article] The minor pilin subunit Sgp2 is necessary for assembly of the pilus encoded by the srtG cluster of Streptococcus suis. (2011)
  • Author(s): Okura, M., Osaki, M., Fittipaldi, N., Gottschalk, M., Sekizaki, T., and Takamatsu, D.
  • Journal Title: J. Bacteriol. Volume: 193 Issue: 4 Pages: 822-831
  • DOI: 10.1128/jb.01555-09
  • Peer Reviewed
• [Journal Article] Lineage and Virulence of Streptococcus suis Serotype 2 Isolates from North America. (2011)
  • Author(s): Fittipaldi, N., Xu, J., Lacouture, S., Tharavichitkul, P., Osaki, M., Sekizaki, T., Takamatsu, D., Gottschalk, M.
  • Journal Title: Emerg. Infect. Dis. Volume: 17 Issue: 12 Pages: 2239-2244
  • DOI: 10.3201/eid1712.110609
  • Peer Reviewed
• [Presentation] Reappraisal of the taxonomy of Streptococcus suis serotypes 20, 22, and 26 based on DNA homology (2014)
  • Author(s): 野本竜平、石田沙倉、遠矢真理、関崎勉、丸山史人、大澤朗
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: タワーホール船堀(国立感染研)
• [Presentation] Streptococcus suisの再分類に対応するPCR法 (2014)
  • Author(s): 石田沙倉、遠矢真理、野本竜平、大澤朗、関崎勉
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: タワーホール船堀(国立感染研)
• [Presentation] 豚レンサ球菌莢膜糖鎖合成遺伝子領域を標的とした型別法の開発と利用 (2014)
  • Author(s): 大倉正稔、大崎慎人、関崎勉、丸山史人、野澤孝志、中川一路、浜田茂幸、高松大輔
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: タワーホール船堀(国立感染研)
• [Presentation] Development of a novel LAMP assay for detection of Streptococcus suis (2014)
  • Author(s): Ishida, S., Tohya, M., Nomoto, R., Osawa, R., and Sekizaki, T.
  • Organizer: 12th Japan-Korea International Symposium on Microbiology 2014
  • Place of Presentation: Tower Hall Funabori, Tokyo, Japan
• [Presentation] Development of a novel LAMP assay for detection of Streptococcus suis. (2014)
  • Author(s): Ishida, S., Tohya, M., Nomoto, R., Osawa, R., and Sekizaki, T.
  • Organizer: 12th Japan-Korea International Symposium on Microbiology 2014
  • Place of Presentation: Tower Hall Funabori, Tokyo
• [Presentation] Reappraisal of the taxonomy of Streptococcus suis serotypes 20, 22, and 26 based on DNA homology. (2014)
  • Author(s): 野本竜平、石田沙倉、遠矢真理、関崎 勉、丸山史人、大澤 朗
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: タワーホール船堀、東京都
• [Presentation] Streptococcus suisの再分類に対応するPCR法 (2014)
  • Author(s): 石田沙倉、遠矢真理、野本竜平、大澤 朗、関崎 勉
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: タワーホール船堀、東京都
• [Presentation] 豚レンサ球菌莢膜糖鎖合成遺伝子領域を標的とした型別法の開発と利用 (2014)
  • Author(s): 大倉正稔、大崎慎人、関崎 勉、丸山史人、野澤孝志、中川一路、浜田茂幸、高松大輔
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: タワーホール船堀、東京都
• [Presentation] 豚レンサ球菌の莢膜と病原性について (2013)
  • Author(s): 関崎勉
  • Organizer: シンポジウム「グローバリズム時代の新興感染症」
  • Place of Presentation: 日本大学生物資源科学部、藤沢、神奈川
• [Presentation] Loss of capsule and an alternative virulence in Streptococcus suis (2013)
  • Author(s): Sekizaki, T.
  • Organizer: First International Workshop on Streptococcus suis
  • Place of Presentation: Beijing, China
• [Presentation] Streptococcus suis全血清型の莢膜多糖合成遺伝子クラスターの解析 (2013)
  • Author(s): 大倉正稔、高松大輔、丸山史人、野澤孝志、中川一路、大﨑慎人、関崎勉、熊谷由美、浜田茂幸
  • Organizer: 第22回Lancefieldレンサ球菌研究会
  • Place of Presentation: 順天堂大学、東京
• [Presentation] Gene conversions in the Streptococcus suis cps genes lead to either death or benefit for adhesiveness of the whole population (2013)
  • Author(s): Lakkitjaroen, N., Takamatsu, D., Okura, M., Sato, M., Osaki, M., and Sekizaki, T.
  • Organizer: 第22回Lancefieldレンサ球菌研究会
  • Place of Presentation: 順天堂大学、東京
• [Presentation] Streptococcus suis全血清型の莢膜関連遺伝子群の解析 (2013)
  • Author(s): 大倉正稔、高松大輔、丸山史人、野澤孝志、中川一路、大﨑慎人、関崎勉、浜田茂幸
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 幕張メッセ、千葉
• [Presentation] Mutations in cps2J and SSU0533-0534 regions of cps2 locus are lethal for Streptococcus suis (2013)
  • Author(s): Nattakan Lakkitjaroen、高松大輔、大倉正稔、大﨑慎人、関崎勉
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 幕張メッセ、千葉
• [Presentation] Loss of capsule and an alternative virulence in Streptococcus suis. (2013)
  • Author(s): Sekizaki, T.
  • Organizer: First International Workshop on Streptococcus suis
  • Place of Presentation: Beijing, China
  • Invited
• [Presentation] Streptococcus suis全血清型の莢膜多糖合成遺伝子クラスターの解析 (2013)
  • Author(s): 大倉正稔、高松大輔、丸山史人、野澤孝志、中川一路、大﨑慎人、関崎 勉、熊谷由美、浜田茂幸
  • Organizer: 第22回Lancefieldレンサ球菌研究会
  • Place of Presentation: ホテル島根イン青山、東京都
• [Presentation] Gene conversions in the Streptococcus suis cps genes lead to either death or benefit for adhesiveness of the whole population. (2013)
  • Author(s): Lakkitjaroen, N., Takamatsu, D., Okura, M., Sato, M., Osaki, M., and Sekizaki, T.
  • Organizer: 第22回Lancefieldレンサ球菌研究会
  • Place of Presentation: ホテル島根イン青山、東京都
• [Presentation] 豚レンサ球菌感染症の分子遺伝学的研究 (2012)
  • Author(s): 関崎勉
  • Organizer: 第5回感染病態研究フロンティア
  • Place of Presentation: 東京
  • Year and Date: 2012-08-04
• [Presentation] Surface components of Streptococcus suis (2012)
  • Author(s): Sekizaki, T.
  • Organizer: 11th Korea-Japan International Symposium on Microbiology 2012
  • Place of Presentation: Buyeo, Korea
• [Presentation] Mutations that affect the production of capsule in Streptococcus suis (2012)
  • Author(s): Lakkitjaroen, N. Takamatsu, D., Okura, M., Osaki, M., Sekizaki, T.
  • Organizer: 第21回Lancefieldレンサ球菌研究会
  • Place of Presentation: 大阪大学、大阪
• [Presentation] ヒトと動物に共通するレンサ球菌感染症(特別講演) (2012)
  • Author(s): 関崎勉
  • Organizer: 第22回Lancefieldレンサ球菌研究会
  • Place of Presentation: 大阪大学、大阪
• [Presentation] Recent research progress in Streptococcus suis infection (2012)
  • Author(s): Sekizaki, T.
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎ブリックホール(長崎大学医学部)、長崎
• [Presentation] Streptococcus suis血清型1型、2型、1/2型及び14型の莢膜合成遺伝子領域の比較解析 (2012)
  • Author(s): 大倉正稔,大崎慎人,関崎勉,高松大輔
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎ブリックホール(長崎大学医学部)、長崎
• [Presentation] Mutations that affect the production of capsule in Streptococcus suis. (2012)
  • Author(s): Nattakan Lakkitjaroen, Daisuke Takamatsu, Masatoshi Okura, Makoto Osaki, Tsutomu Sekizaki.
  • Organizer: 第21回Lancefieldレンサ球菌研究会
  • Place of Presentation: 大阪大学、大阪府
• [Presentation] ヒトと動物に共通するレンサ球菌感染症 (2012)
  • Author(s): 関崎 勉
  • Organizer: 第21回Lancefieldレンサ球菌研究会
  • Place of Presentation: 大阪大学、大阪府
  • Invited
• [Presentation] Mutations in the mutational cold regions of the cps locus are lethal for Streptococcus suis (2012)
  • Author(s): Nattakan Lakkitjaroen, Daisuke Takamatsu, Masatoshi Okura, Makoto Osaki, Tsutomu Sekizaki
  • Organizer: 第８６回日本細菌学会総会
  • Place of Presentation: 幕張メッセ、千葉県
• [Presentation] Streptococcus suis全血清型の莢膜関連遺伝子群の解析 (2012)
  • Author(s): 大倉正稔、高松大輔、丸山史人、野澤孝志、中川一路、大崎慎人、関崎勉、浜田茂幸
  • Organizer: 第８６回日本細菌学会総会
  • Place of Presentation: 幕張メッセ、千葉県
• [Presentation] Recent research progress in Streptococcus suis infection. (2012)
  • Author(s): Tsutomu Sekizaki.
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎ブリックホール
• [Presentation] Frequent loss of capsular expression in Streptococcus suis isolates from porcine endocarditis and its significance in the pathogenesis of endocarditis (2011)
  • Author(s): Lakkitjaroen, N., Takamatsu, D., Okura, M., Sato, M., Osaki, M., Sekizaki, T.
  • Organizer: International Union of Microbial Society 2011
  • Place of Presentation: Sapporo, Japan
• [Presentation] Frequent loss of capsular expression in Streptococcus suis isolates from porcine endocarditis and its significance in the pathogenesis of endocarditis (2011)
  • Author(s): Lakkitjaroen, L., Takamatsu, D., Okura, M., Sato, M., Osaki, M., Sekizaki, T.
  • Organizer: 第20回Lancefieldレンサ球菌研究会
  • Place of Presentation: 愛知学院大学,名古屋
• [Presentation] Frequent loss of capsular expression in Streptococcus suis isolates from porcine endocarditis and its significance in the pathogenesis of endocarditis. (2011)
  • Author(s): Nattakan Lakkitjaroen, Daisuke Takamatsu, Masatoshi Okura, Masumi Sato, Makoto Osaki, Tsutomu Sekizaki.
  • Organizer: International Union of Microbial Society 2011
  • Place of Presentation: Sapporo, Japan.
• [Presentation] Frequent loss of capsular expression in Streptococcus suis isolates from porcine endocarditis and its significance in the pathogenesis of endocarditis. (2011)
  • Author(s): Nattakan Lakkitjaroen, Daisuke Takamatsu, Masatoshi Okura, Masumi Sato, Makoto Osaki, Tsutomu Sekizaki.
  • Organizer: 第20回Lancefieldレンサ球菌研究会
  • Place of Presentation: 愛知学院大学
• [Presentation] Surface components of Streptococcus suis.
  • Author(s): Sekizaki, T.
  • Organizer: 11th Korea-Japan International Symposium on Microbiology
  • Place of Presentation: Buyeo, Korea
  • Invited
• [Presentation] 豚レンサ球菌感染症の分子遺伝学的研究
  • Author(s): 関崎 勉
  • Organizer: 第5回感染病態研究フロンティア
  • Place of Presentation: 両国第一ホテル、東京都
  • Invited
• [Presentation] 豚レンサ球菌の表層タンパクと病原性
  • Author(s): 関崎 勉
  • Organizer: 第11回微生物研究会
  • Place of Presentation: 東京大学、東京都
  • Invited
• [Book] 「昨今の食中毒問題とその規制強化から学ぶもの」"生食のおいしさとリスク"一色賢司編 (2013)
  • Author(s): 関崎勉
  • Publisher: エヌ・ティー・エス出版
• [Book] 一色賢司編 「昨今の食中毒問題とその規制強化から学ぶもの」、”生食のおいしさとリスク” (2013)
  • Author(s): 関崎 勉
  • Total Pages: 7
  • Publisher: エヌ・ティー・エス出版