| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
The salivary gland transcriptome analysis of Lutzomyia ayacuchensis, a vector of cutaneous leishmaniasis in Ecuadorian Andes, was performed and abundant transcripts coding for secreted proteins were identified. The most abundant transcript coded for an RGD-containing peptide was expressed in Escherichia coli and its activity was characterized. The resulting protein inhibited both platelet aggregation and blood coagulation pathway, indicating that the peptide plays an important role in the blood feeding of Lu. ayacuchensis by inhibiting host hemostasis via dual mechanisms. On the other hand, salivary nucleotidases of sand flies were assessed on their activity as an exacerbation factor for Leishmania infection. Salivary nucleotidases, PduApy and PduADA, slightly accelerated the disease progression after co-injection of Leishmania parasites, suggesting that these proteins in saliva may be partly associated with exacerbation of the disease in leishmaniasis.
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