Persistent infection and sequence analysis of PRRS virus in the southern Kyushu

• Project/Area Number: 23580425
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: University of Miyazaki
• Principal Investigator: HIRAI Takuya 宮崎大学, 農学部, 准教授 (60321668)
• Co-Investigator(Kenkyū-buntansha): YAMAGUCHI Ryoji 宮崎大学, 農学部, 教授 (90150169)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 豚 / 豚繁殖・呼吸障害症候群 / 南九州 / ウイルス / 持続感染 / 病理学

Outline of Final Research Achievements

The objectives of the present study were to evaluate the anatomic localization of porcine reproductive and respiratory syndrome virus (PRRSV) in naturally infected pigs and to determine whether oral fluid could be used to detect the virus in infected animals. Our results confirm previous findings that PRRSV primarily replicates in tonsils and is then shed into oral fluid. Therefore, oral fluid sampling may be effective for the surveillance of PRRSV in breeding herds.

Research Products

• [Journal Article] Enhanced detection of Porcine reproductive and respiratory syndrome virus in fixed tissues by in situ hybridization following tyramide signal amplification. (2015)
  • Author(s): Trang NT, Hirai T, Ngan PH, Lan NT, Fuke N, Toyama K, Yamamoto T, Yamaguchi R.
  • Journal Title: J Vet Diagn Invest Volume: in press
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Detection of porcine reproductive and respiratory syndrome virus in oral fluid from naturally infected pigs in a breeding herd. (2014)
  • Author(s): Trang NT, Hirai T, Yamamoto T, Matsuda M, Okumura N, Giang NT, Lan NT,
  • Journal Title: J Vet Sci Volume: 15 Pages: 361-7
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Oral fluid for detection of porcine reproductive and respiratory syndrome virus in naturally infected pigs in breeding herd (2014)
  • Author(s): Trang NT, Hirai T, Yamamoto T, Matsuda M, Okumura N, Giang NTH, Lan NT,Yamaguchi R
  • Journal Title: J Vet Sci Volume: in press
  • Peer Reviewed
• [Presentation] 子豚のPRRSコントロール法を検討するためのウイルス感染状況調査 (2014)
  • Author(s): 兒玉亜侑美、平井卓哉、奥村尚子、兼光宏枝、小東智哉、山口良二
  • Organizer: 第63回 九州地区獣医師大会・平成26年度
  • Place of Presentation: 鹿児島市
  • Year and Date: 2014-10-14
• [Presentation] 子豚の口腔液中で検出される豚繁殖・呼吸障害症候群ウイルスの局在部位に関する免疫組織化学的研究 (2013)
  • Author(s): 山本 司 平井 卓哉 山口 良二
  • Organizer: 第156回 日本獣医学会学術集会
  • Place of Presentation: 岐阜市
• [Presentation] Localization of porcine reproductive and respiratory syndrome virus in naturally infected sows (2013)
  • Author(s): TRANG NT, HIRAI T, YAMAGUCHI R
  • Organizer: 第156回 日本獣医学会学術集会
  • Place of Presentation: 岐阜市
• [Presentation] A field Investigation: Localization of porcine reproductive and respiratory syndrome virus in naturally infected sows and piglets (2013)
  • Author(s): Takuya Hirai
  • Organizer: 3rd International symposium of University of Miyazaki
  • Place of Presentation: 宮崎市
• [Presentation] 死亡事故率の高い一養豚場における発育不良豚についての病理学的検索 (2013)
  • Author(s): 渡山恵子、平井卓哉、山口良二
  • Organizer: 第62回 九州地区獣医師大会・平成25年度 獣医学術九州地区大会
  • Place of Presentation: 大分市
• [Presentation] 宮崎県内の一養豚場におけるPRRS馴致法の検討 (2013)
  • Author(s): 奥村尚子、平井卓哉、小東智哉、山口良二
  • Organizer: 第62回 九州地区獣医師大会・平成25年度 獣医学術九州地区大会
  • Place of Presentation: 大分市
• [Presentation] 流産母豚における豚繁殖・呼吸障害症候群ウイルスの局在 (2012)
  • Author(s): Nguyen Thi TRANG 、松田麻里、平井卓哉、山口良二
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手大学