| Project/Area Number |
23580426
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Kagoshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OKAMOTO Karoku 鹿児島大学, 共同獣医学部, 教授 (00136847)
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| Project Period (FY) |
2011 – 2013
|
| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | サルモネラ / 抗菌剤耐性 / ブロイラー |
|---|
| Research Abstract |
The study was conducted to determine the antimicrobial resistance and characterize the B-lactamase genes and the plasmids in S. Infantis isolates from broilers. In 93 (6.3%) isolates recovered, 33 (35.5%) isolates showed resistance to cefotaxime conferred by TEM-20, TEM-52 and CTX-M-25 ESBLs. In addition to ESC-resistance, eight (8.6%) isolates exhibited resistance to cefoxitin mediated by CMY-2 AmpC B-lactamase. Plasmid analysis revealed the blaTEM-20 and blaCMY2 genes were associated with IncP plasmids, blaTEM was -52 linked with a non-typable plasmid and blaCTX-M-25 was carried by an IncA/C plasmid. Resistance to streptomycin, sulfamethoxazole, and oxytetracycline encoded by the aadA1, sul1, and tetA, respectively, was found in 86 (92.5%) isolates. These data indicate that S. Infantis isolates producing ESBLs and AmpC B-lactamase have spread among broiler farms in Japan. These data demonstrated the incidence of ESC-resistant S. Infantis carrying blaTEM-52 remarkably increased.
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