Analysis of mechanism for the acquisition of infectivity by avian influenza virus in chickens

• Project/Area Number: 23580432
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: Kyoto Sangyo University
• Principal Investigator: TAKAKUWA Hiroki 京都産業大学, 総合生命科学部, 准教授 (20449507)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 鳥インフルエンザウイルス / 宿主域 / 感受性 / 鳥ｲﾝﾌﾙｴﾝｻﾞｳｲﾙｽ

Research Abstract

To provide information on the mechanism of acquisition of infectivity by avian influenza virus in chickens, we compared the complete sequences of H3N8 virus isolated from chickens and ducks. Seven amino acid substitutions were detected in the hemagglutinin (HA), matrix 2 (M2), and nonstructural 2 (NS2) proteins. Especially, three substitutions were detected at the position of 128, 137, and 145 on the globular head in the HA protein. These results suggest that these three amino acids in HA protein are related to the infectivity of avian influenza virus in chickens. Further studies using reverse genetics procedures, the HA genes of chicken and duck recombinant avian influenza virus strains were exchanged, are therefore necessary to clarify this issue.

Research Products

• [Journal Article] Molecular epidemiology of avian influenza viruses circulating among healthy poultry flocks in farms in northern Vietnam (2012)
  • Author(s): H. Takakuwa, T. Yamashiro, M. Q. Le, L. S. Phuong, H. Ozaki, R. Tsunekuni, T. Usui, H. Ito, M. Morimatsu, Y. Tomioka, T. Yamaguchi, T. Ito, T. Murase, E. Ono, K. Otsuki
  • Journal Title: Preventive Veterinary Medicine Volume: Vol.103 Pages: 192-200
  • NAID: 120006985204
  • Peer Reviewed
• [Journal Article] Isolation and characterization of H6N1 and H9N2 avian influenza viruses from ducks in hanoi (2012)
  • Author(s): K. Hotta, H. Takakuwa, Q. M. T. Le, S. L. Phuong, T. Murase, E. Ono, T. Ito, K. Otsuki, and T. Yamashiro
  • Journal Title: Vietnam., Virus Research Volume: Vol.163 Pages: 448-453
  • Peer Reviewed
• [Journal Article] Molecular epidemiology of avian influenza viruses circulating among healthy poultry flocks in farms in northern Vietnam (2012)
  • Author(s): Takakuwa, H., et al.
  • Journal Title: Preventive Veterinary Medicine Volume: 103 Issue: 2-3 Pages: 192-200
  • DOI: 10.1016/j.prevetmed.2011.09.014
  • Peer Reviewed
• [Journal Article] Isolation and characterization of H6N1 and H9N2 avian influenza viruses from Ducks in Hanoi, Vietnam (2012)
  • Author(s): Hotta, K.
  • Journal Title: Virus Res. Volume: 163(2) Issue: 2 Pages: 448-453
  • DOI: 10.1016/j.virusres.2011.11.004
  • Peer Reviewed
• [Presentation] Molecular epidemiology of avian influenza viruses circulating among healthy duck flocks in farms in northern Vietnam during year 2006-2010 (2012)
  • Author(s): H. Takakuwa, T. Usui, K. Hotta, M. Q. Le, L. S. Phuong, R. Tsunekuni, H. Ozaki, H. Ito, M. Morimatsu, Y. Tomioka, T. Yamaguchi, T. Ito, T. Murase, E. Ono, T. Yamashiro, K. Otsuki
  • Organizer: アジアリサーチフォーラム
  • Place of Presentation: 神戸
• [Presentation] Molecular epidemiology of avian influenza viruses circulating among healthy duck flocks in farms in northern Vietnam during year 2006-2010 (2012)
  • Author(s): H. Takakuwa, T. Usui, K. Hotta, M. Q. Le, L. S. Phuong, R. Tsunekuni, H. Ozaki, H. Ito, M. Morimatsu, Y. Tomioka, T. Yamaguchi, T. Ito, T. Murase, E. Ono, T. Yamashiro, K. Otsuki
  • Organizer: J-GRIDのアジア・アフリカリサーチフォーラム
  • Place of Presentation: 神戸
• [Remarks]
  • URL: http://www.kyoto-su.ac.jp/graduate/g_eng/g_bio/kyoin/takakuwa.html