Establishment of novel and objective ultrasonographic examination and its application to clinical veterinary medicine

• Project/Area Number: 23580436
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: Hokkaido University
• Principal Investigator: TAKIGUCHI MITSUYOSHI 北海道大学, (連合)獣医学研究科, 教授 (70261336)
• Co-Investigator(Kenkyū-buntansha): YAMASAKI Masahiro 北海道大学, 大学院獣医学研究科, 准教授 (40322846) ; OHTA Hiroshi 北海道大学, 大学院獣医学研究科, 助教 (50431333)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 超音波検査 / 造影超音波検査 / 膵臓 / 犬 / 膵炎 / 超音波造影剤 / ソナゾイド / 超音波診断

Research Abstract

To establish novel and objective ultrasonographic examination and apply it to clinical veterinary medicine, fundamental parameters of normal canine pancreatic perfusion were determined by using contrast agent Sonazoid. Then, novel diagnostic critetria for acute pancreatitis focused on pancreatic perfusion changes was established based on analysis of contrast-enhanced ultrasonographic examination in experimentally induced acute pancreatitis model in the dog.
In addition, left atrial phasic function in healthy dogs by means of 2-D speckle tracking echocardiography with time-left atrial area curve analysis was evaluated. The repeatability and reproducibility of obtained measurements were adequate. This results indicates that studies are needed to determine clinical applicability in canine patients.

Research Products

• [Journal Article] Qualitative and quantitative contrast-enhanced ultrasonographic assessment of cerulein-induced acute pancreatitis in dogs. (2014)
  • Author(s): Lim SY, Nakamura K, Morishita K, Sasaki N, Murakami M, Osuga T, Ohta H, Yamasaki M, Takiguchi M
  • Journal Title: Journal of Veterinary Internal Medicine Volume: 28 Issue: 2 Pages: 496-503
  • DOI: 10.1111/jvim.12319
  • Peer Reviewed / Open Access
• [Journal Article] Ultrasound image-guided therapy enhances antitumor effect of cisplatin (2013)
  • Author(s): Sasaki N, Kudo N, Nakamura K, Lima SY, Murakami M, Kumara WRB, Tamura Y, Ohta H, Yamasaki M, Takiguchi M.
  • Journal Title: Journal of Medical Ultrasonics Volume: 41 Issue: 1 Pages: 11-21
  • DOI: 10.1007/s10396-013-0475-y
  • Peer Reviewed
• [Journal Article] Qualitative and Quantitative Contrast Enhanced Ultrasonography of the Pancreas Using Bolus Injection and Continuous Infusion Methods in Normal Dogs (2013)
  • Author(s): Lim SY, Nakamura K, Morishita K, Sasaki N, Murakami M, Osuga T, Ohta H, Yamasaki M, Takiguchi M.
  • Journal Title: Journal of Veterinary Medical Science Volume: 75 Issue: 12 Pages: 1601-1607
  • DOI: 10.1292/jvms.13-0199
  • NAID: 130003362277
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] Repeatability and reproducibility of measurements obtained via two-dimensional speckle tracking echocardiography of the left atrium and time-left atrial area curve analysis in healthy dogs (2013)
  • Author(s): Osuga T, Nakamura K, Lim SY, Tamura Y, Rajapakshage BKW, Murakami M, Sasaki N, Morishita K, Ohta H, Yamasaki M, Takiguchi M.
  • Journal Title: Am J Vet Res Volume: vol.74 Pages: 864-869
  • Peer Reviewed
• [Journal Article] Repeatability and reproducibility of measurements obtained via two-dimensional speckle tracking echocardiography of the left atrium and time-left atrial area curve analysis in healthy dogs. (2013)
  • Author(s): Osuga T, Nakamura K, Lim SY, Tamura Y, Rajapakshage BKW, Murakami M, Sasaki N, Morishita K, Ohta H, Yamasaki M, Takiguchi M
  • Journal Title: Am J Vet Res Volume: 74 Pages: 1111-1111
  • Peer Reviewed
• [Journal Article] Activation of microbubbles by short-pulsed ultrasound enhances the cytotoxic effect of cis-diamminedichloroplatinum (II) in a canine thyroid adenocarcinoma cell line in vitro (2012)
  • Author(s): Sasaki N, Nakamura K, et al
  • Journal Title: Ultrasound in Medicine and Biology Volume: 38 Issue: 1 Pages: 109-118
  • DOI: 10.1016/j.ultrasmedbio.2011.09.017
  • Peer Reviewed
• [Journal Article] Activation of microbubbles by short-pulsed ultrasound enhances the cytotoxic effect of cis-diamminedichloroplatinum (II) in a canine thyroid adenocarcinoma cell line in vitro (2012)
  • Author(s): Sasaki N, Kudo N, Nakamura K, Lim SY, Murakami M, Bandula Kumara WR, Tamura Y, Ohta H, Yamasaki M, Takiguchi M.
  • Journal Title: Ultrasound Med Biol Volume: 38 Pages: 109-118
  • Peer Reviewed
• [Presentation] Quantitative contrast-enhanced ultrasonographic assessment of naturally occurring pancreatitis in dogs (2014)
  • Author(s): Lim SY, Nakamura K, Morishita K, Suzuki S, Ohta H, Yamasaki M, Takiguchi M.
  • Organizer: 2014 ACVIM Forum
  • Place of Presentation: Gaylord Opryland Resort and Convention Center (Nashville, USA)
• [Presentation] Quantitative contrast-enhanced ultrasonographic assessment of naturally occurring pancreatitis in dogs (2014)
  • Author(s): Lim SY, Nakamura K, Morishita K, Ohta H, Suzuki S, Yamasaki M, Takiguchi M.
  • Organizer: JCVIM Forum
  • Place of Presentation: パシフィコ横浜(横浜市)
• [Presentation] Quantitative Contrast-enhanced Ultrasonographic Assessment of Naturally Occurring Pancreatitis in Dogs (2014)
  • Author(s): Lim SY, Nakamura K, Morishita K, Ohta H, Suzuki S, Yamasaki M, Takiguchi M.
  • Organizer: 日本獣医内科学アカデミー第10回記念学術大会
  • Place of Presentation: パシフィコ横浜（横浜市）
• [Presentation] QUANTITATIVE CONTRAST-ENHANCED ULTRASONOGRAPHIC ASSESSMENT OF NATURALLY OCCURRING PANCREATITIS IN DOGS (2014)
  • Author(s): Lim SY, Nakamura K, Morishita K, Suzuki S, Ohta H, Yamasaki M, Takiguchi M.
  • Organizer: 2014 ACVIM Forum
  • Place of Presentation: Gaylord Opryland Resort and Convention Center (Nashville, USA)
• [Presentation] Qualitative and quantitative contrast enhanced ultrasonographic assessment of cerulein-induced pancreatitis in dogs (2013)
  • Author(s): Lim SY, Nakamura K, Morishita K, Sasaki N, Murakami M, Osuga T, Ohta H, Yamasaki M, Takiguchi M.
  • Organizer: 2013 ACVIM Forum
  • Place of Presentation: Washington State Convention Center (Seattle, USA)
• [Presentation] Qualitative and quantitative contrast enhanced ultrasonographic assessment of cerulein- induced pancreatitis in dogs (2013)
  • Author(s): Lim SY, Nakamura K, Morishita K, Ohta H, Yamasaki M, Takiguchi M.
  • Organizer: JCVIM Forum
  • Place of Presentation: パシフィコ横浜(横浜市)
• [Presentation] QUALITATIVE AND QUANTITATIVE CONTRAST ENHANCED ULTRASONOGRAPHIC ASSESSMENT OF CERULEIN-INDUCED PANCREATITIS IN DOGS (2013)
  • Author(s): Lim SY, Nakamura K, Morishita K, Ohta H, Yamasaki M, Takiguchi M.
  • Organizer: 2013 ACVIM Forum
  • Place of Presentation: Washington State Convention Center (Seattle, USA)
• [Presentation] Qualitative and quantitative contrast enhanced ultrasonographic assessment of cerulein-induced pancreatitis in dogs (2013)
  • Author(s): Sue Yee Lim, Nakamura K, Morishita K, Ohta H, Yamasaki M, Takiguchi M
  • Organizer: Japanese College of Veterinay Internal Medicine
  • Place of Presentation: パシフィコ横浜（横浜市）
• [Presentation] ソナゾイド造影超音波検査と機械学習を組み合わせた脾臓腫瘤の自動良悪性鑑別の試み (2012)
  • Author(s): 中村健介,高木一也,近藤敏志,滝口満喜
  • Organizer: 日本獣医画像診断学会
  • Place of Presentation: 岩手大学(盛岡市)
  • Year and Date: 2012-09-16
• [Presentation] Quantitative comparison of bolus versus continuous infusion contrast enhanced ultrasonography of pancreas in normal dogs (2012)
  • Author(s): Lim SY, Nakamura K, Ohta H, Yamasaki M, Takiguchi M.
  • Organizer: 2012 International veterinary radiology association meeting
  • Place of Presentation: Almira Hotel (Bursa, Turkey)
• [Presentation] Quantitative comparison of bolus versus continuous infusion contrast enhanced ultrasonography of pancreas in normal dogs (2012)
  • Author(s): Sue Yee Lim, Nakamura K, Takiguchi M
  • Organizer: International Vetrinary Radiology Association Meeting
  • Place of Presentation: Almira Hotel (Turkey)
• [Presentation] ソナゾイド造影超音波検査と機械学習を組み合わせた脾臓腫瘤の自動良悪性鑑別の試み (2012)
  • Author(s): 中村健介、滝口満喜、高木一也、近藤敏志
  • Organizer: 日本獣医画像診断学会
  • Place of Presentation: 岩手大学（盛岡市）
• [Patent(Industrial Property Rights)] 医用データ処理装置、医用データ処理方法、及び超音波診断装置 (2013)
  • Inventor(s): 高木一也、滝 口満喜、中村健介
  • Industrial Property Rights Holder: パナソニック株式会社
  • Industrial Property Rights Type: 特許
  • Filing Date: 2013-08-02
• [Patent(Industrial Property Rights)] 超音波診断装置、超音波診断方法 (2012)
  • Inventor(s): 滝口満喜、中村健介、高木一也
  • Industrial Property Rights Holder: 滝口満喜、中村健介、高木一也
  • Industrial Property Rights Type: 特許
  • Filing Date: 2012-08-07