Study on molecular basis of growth factor/receptor of canine hemangiosarcoma

• Project/Area Number: 23580439
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: Gifu University
• Principal Investigator: SAKAI Hiroki 岐阜大学, 応用生物科学部, 准教授 (40283288)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
• Keywords: 血管肉腫 / 犬 / 培養細胞株 / mTOR / PDGFR / DNAアレイ / PDGF受容体 / 遺伝子変異 / MUC-1 / 株化細胞 / ｍTOR / Akt / Erk / シグナル伝達系 / 増殖因子 / ヌードマウス / 移植 / 細胞株

Research Abstract

Seven canine hemangiosarcoma cell lines were established for analysis of pathogenesis of hemangiosarcoma. All cell lines showed active intake of DiI-Ac-LDL, and positive for CD31, VEGF-A, bFGF, HGF, IGF-I and PDGF-B mRNA by RT-PCR. Constitutive activation of mTORC2/Akt/4E-BP1 associated with the proliferation of cell lines in vitro and in vivo, and this activation was confirmed in spontaneous canine hemangiosarcomas. Moreover, the high expression of PDGF receptor beta without genomic mutation of jaxutamembrane area was detected in hemangiosarcoma. It is suggested that mTOR and PDGF receptor beta may be considered as one of therapeutic targets.

Research Products

• [Journal Article] Analysis of genomic mutation and immunohistochemistry of platelet-derived growth factor receptors in canine vascular tumours (2014)
  • Author(s): Abou Asa, S., Mori, T., Maruo, K., Khater, A., El-Sawa,k A., Abd El-Aziz, E., Yanai, T., and Sakai, H.
  • Journal Title: Vet. Comp. Oncol Volume: (in press)
  • Peer Reviewed
• [Journal Article] ミニチュア・ダックスフンドの炎症性結直腸ポリープの細胞学的特徴と多核巨細胞の診断的意義 (2014)
  • Author(s): 井上紗季,酒井洋樹,米丸加余子,柳井徳磨
  • Journal Title: 日本獣医師会雑誌 Volume: 67 Pages: 193-198
  • NAID: 130004640754
  • Peer Reviewed
• [Journal Article] Analysis of genomic mutation and immunohistochemistry of platelet-derived growth factor receptors in canine vascular tumours. (2014)
  • Author(s): Abou Asa, S., Mori, T., Maruo, K., Khater, A., El-Sawa,k A., Abd El-Aziz, E., Yanai, T., and Sakai, H.
  • Journal Title: Vet. Comp. Oncol. Volume: in press Issue: 3 Pages: 237-245
  • DOI: 10.1111/vco.12035
  • Peer Reviewed
• [Journal Article] Polypiod eosinophilic ureteritis in a dog (2013)
  • Author(s): Sakai H., Hajiri, Y., Kobayashi, M., Ohta, G., and Yanai, T.
  • Journal Title: Aust. Vet. Practitioner Volume: 43 Pages: 527-529
  • Peer Reviewed
• [Journal Article] Chemically Modified Synthetic microRNA-205 Inhibits the Growth of Melanoma Cells In Vitro and In Vivo (2013)
  • Author(s): Noguchi, S., Iwasaki, J., Kumazaki, M., Mori, T., Maruo, K., Saka,i H., Yamada, N., Shimada, K., Naoe, T., Kitade, Y., and Akao, Y.
  • Journal Title: Mol. Ther Volume: 21(6) Pages: 1204-11
  • Peer Reviewed
• [Journal Article] The Class A macrophage scavenger receptor CD204 is a useful immunohistochemical marker of canine histiocytic sarcoma (2013)
  • Author(s): Kato, Y., Murakami, M., Hoshino, Y., Mori, T., Maruo, K., Hirata, A., Nakagawa, T. L. D. R., Yanai, T., and Sakai, H.
  • Journal Title: J. Comp. Pathol Volume: 148 Pages: 188-96
  • Peer Reviewed
• [Journal Article] The class A macrophage scavenger receptor CD204 is a useful immunohistochemical marker of canine histiocytic sarcoma. (2013)
  • Author(s): Kato Y, Murakami M, Hoshino Y, Mori T, Maruo K, Hirata A, Nakagawa TL, Yanai
  • Journal Title: J Comp Pathol Volume: 148 Issue: 2-3 Pages: 188-196
  • DOI: 10.1016/j.jcpa.2012.06.009
  • Peer Reviewed
• [Journal Article] Chemically Modified Synthetic microRNA-205 Inhibits the Growth of Melanoma Cells In Vitro and In Vivo. (2013)
  • Author(s): Noguchi, S., Iwasaki, J., Kumazaki, M., Mori, T., Maruo, K., Saka,i H., Yamada, N., Shimada, K., Naoe, T., Kitade, Y., and Akao, Y.
  • Journal Title: Mol. Ther. Volume: 21 Issue: 6 Pages: 1204-1211
  • DOI: 10.1038/mt.2013.70
  • Peer Reviewed
• [Journal Article] Multimodal therapy for the treatment of an oncocytoma in the nasal cavity of a cat. (2013)
  • Author(s): Mori T, Mishima H, Sakai H, Noguchi S, Maruo K.
  • Journal Title: J Small Anim Pract Volume: 54 Issue: 7 Pages: 390-390
  • DOI: 10.1111/jsap.12094
  • Peer Reviewed
• [Journal Article] Polypiod eosinophilic ureteritis in a dog. (2013)
  • Author(s): Sakai H., Hajiri, Y., Kobayashi, M., Ohta, G., and Yanai, T.
  • Journal Title: Aust. Vet. Practitioner Volume: 43 Pages: 527-529
  • Peer Reviewed
• [Journal Article] ImmunohistochemicalAnalysis of the Akt/mTOR/4E-BP1 signalling pathway in canine haemangiomas and haemangiosarcomas (2012)
  • Author(s): Murai, A., Abou, A. S., Kodama, A., Sakai, H., Hirata, A., Yanai, T.
  • Journal Title: J. Comp. Pathol Volume: 147 Pages: 430-440
  • Peer Reviewed
• [Journal Article] Fascin-1 expression in canine cutaneous and oral melanocytic tumours (2012)
  • Author(s): Yamada, N., Mori, T., Murakami, M., Noguchi, S., Sakai, H., Akao, Y., Maruo, K.
  • Journal Title: Vet. Comp. Oncol Volume: 10 Pages: 303-311
  • Peer Reviewed
• [Journal Article] Constitutive phosphorylation of the mTORC2/Akt/4E-BP1 pathway in newly derived canine hemangiosarcoma cell lines (2012)
  • Author(s): Murai, A., Asa, S. A., Kodama, A., Hirata, A., Yanai, T., and Sakai, H.
  • Journal Title: BMC Vet. Res Volume: 8 Pages: 128-128
  • Peer Reviewed
• [Journal Article] Cytologically atypical anal sac gland carcinoma in a dog (2012)
  • Author(s): Sakai, H., Murakami, M., Mishima, H., Hoshino, Y., Mori, T., Maruo, K. and Yanai, T.
  • Journal Title: Vet. Clin. Pathol Volume: 41 Pages: 291-294
  • Peer Reviewed
• [Journal Article] Palliative radiation therapy in a dog with malignant trichoepithelioma (2012)
  • Author(s): Hoshino, Y., Mori, T., Sakai, H., Murakami, M., and Maruo, K.
  • Journal Title: Aust. Vet. J Volume: 90 Pages: 210-213
  • Peer Reviewed
• [Journal Article] Expression of platelet-derived growth factor and its receptors in spontaneous canine hemangiosarcoma and cutaneous hemangioma (2012)
  • Author(s): Abou Asa, S., Murai, A., Murakami, M., Hoshino, Y., Mori, T., Maruo, K., Khater, A., El-sawak, A., Abd el-Aziz, E., Yanai, T., and Sakai, H.
  • Journal Title: Histol. Histopathol Volume: 27 Pages: 601-607
  • Peer Reviewed
• [Journal Article] Constitutive phosphorylation of the mTORC2/Akt/4E-BP 1 pathway in newly derived canine hemangiosarcoma cell lines (2012)
  • Author(s): Murai, A., Asa, SA., Kodama , A., Hirata, A., Yanai, T. and Sakai, H.
  • Journal Title: BMC Veterinary Research Volume: 8 Issue: 1 Pages: 128-128
  • DOI: 10.1186/1746-6148-8-128
  • Peer Reviewed
• [Journal Article] Immunohistochemical analysis of the Akt/mTOR/4E-BP 1 signalling pathway in canine haemangiomas and haemangiosarcomas (2012)
  • Author(s): Murai, A., Asa, SA., Kodama, A., Sakai, H., Hirata, A. and Yanai , T.
  • Journal Title: Journal of Comparative Pathology Volume: 147 Issue: 4 Pages: 430-440
  • DOI: 10.1016/j.jcpa.2012.05.002
  • Peer Reviewed
• [Journal Article] Expression of platelet-derived growth factor and its receptors in spontaneous canine hemangiosarcoma and cutaneous hemangioma. (2012)
  • Author(s): Asa SA, Murai A, Murakami M, Hoshino Y, Mori T, Maruo K, Khater A, El-Sawak A, El-Aziz EA, Yanai T, Sakai H.
  • Journal Title: Histology and Histopathology Volume: 27 Pages: 601-607
  • Peer Reviewed
• [Journal Article] 犬の鼻腔内に発生した血管肉腫の1例 (2011)
  • Author(s): 関根一哉,星野有希,森崇,酒井洋樹,野口俊助,山田名美,山瀬新悟,丸尾幸嗣
  • Journal Title: 日本獣医師会雑誌 Volume: 64 Pages: 893-895
  • NAID: 10030288496
  • Peer Reviewed
• [Journal Article] Ganglioneuroma in the urinary bladder of a dog (2011)
  • Author(s): Sakai, H., Yonemaru, K., Takeda, M., Niimi, K., Murakami, M., Hirata, A., and Yanai, T.
  • Journal Title: J. Vet. Med. Sci Volume: 73 Pages: 801-803
  • NAID: 130000444834
  • Peer Reviewed
• [Presentation] 腫瘍の細胞診 ～「わかること」と「わからないこと」～ (2014)
  • Author(s): 酒井洋樹
  • Organizer: 日本獣医内科学アカデミー第10回記念学術大会
  • Place of Presentation: パシフィコ横浜
  • Invited
• [Presentation] 耳の腫瘍診断と腫瘍特徴の解説 (2013)
  • Author(s): 酒井洋樹
  • Organizer: 第34回動物臨床医学会年次大会
  • Place of Presentation: 大阪
  • Year and Date: 2013-11-15
• [Presentation] 岐阜県におけるイヌの飼育頭数に対する腫瘍の発生比率の分析 (2013)
  • Author(s): 駒澤敏,酒井洋樹,森崇,村上麻美,伊藤祐典,山崎美史,丸尾幸嗣
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜
  • Year and Date: 2013-09-21
• [Presentation] MRIによりメラノーマと誤診した虹彩血管肉腫の一例 (2013)
  • Author(s): 村上麻美,森崇,伊藤祐典,酒井洋樹,山瀬新悟,川部美史,丸尾幸嗣
  • Organizer: 第54回日本獣医画像診断学会
  • Place of Presentation: 岐阜
  • Year and Date: 2013-09-20
• [Presentation] 岐阜県におけるイヌの飼育頭数に対する腫瘍の発生比率の分析 (2013)
  • Author(s): 駒澤 敏, 酒井 洋樹, 森 崇, 村上 麻美, 伊藤 祐典, 山崎 美史, 丸尾 幸嗣
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学
• [Presentation] 耳の腫瘍 診断と腫瘍特徴の解説 (2013)
  • Author(s): 酒井 洋樹
  • Organizer: 第34動物臨床医学会年次大会
  • Place of Presentation: 大阪国際会議場
  • Invited
• [Presentation] 坐骨神経に発生した組織球肉腫の犬の一例 (2013)
  • Author(s): 13. 淺田 慎也, 高村 文子, 吉田 俊一, 酒井 洋樹, 前田 貞俊, 神志那 弘明
  • Organizer: 第54回日本獣医画像診断学会
  • Place of Presentation: 岐阜大学
• [Presentation] MRIによりメラノーマと誤診した虹彩血管肉腫の一例 (2013)
  • Author(s): 村上 麻美, 森 崇, 伊藤 祐典, 酒井 洋樹, 山瀬 新悟, 川部 美史, 丸尾 幸嗣
  • Organizer: 第54回日本獣医画像診断学会
  • Place of Presentation: 岐阜大学
• [Presentation] 分子標的治療を目指した犬膀胱移行上皮癌細胞株の樹立 (2012)
  • Author(s): 星野有希,森崇,西谷由莉,酒井洋樹,奥村正裕,丸尾幸嗣
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 盛岡
• [Presentation] 犬組織球肉腫におけるMatrix metalloproteinase(MMP)の免疫組織学的検索, 日本獣医学会学術集会講演要旨集 (2012)
  • Author(s): 船戸里紗,米丸加余子,村上麻美,森崇,丸尾幸嗣,平田暁大,柳井徳麿,酒井洋樹
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 盛岡
• [Presentation] 血管系腫瘍におけるAkt/mammalian target of rapamycin(mTOR)/eIF 4E-binding protein 1(4EBP1)の免疫組織学的解析 (2012)
  • Author(s): 村井厚子, Samah Salem,平田暁大,酒井洋樹,柳井徳磨
  • Organizer: 第153回日本獣医学会学術集会
  • Place of Presentation: 東京
• [Presentation] 細胞診標本を用いたCD204に対する免疫染色による犬組織球肉腫の診断 (2012)
  • Author(s): 加藤祐樹,酒井洋樹,平田暁大,森崇,丸尾幸嗣,村上麻美,柳井徳磨
  • Organizer: 第153回日本獣医学会学術集会
  • Place of Presentation: 東京
• [Presentation] 犬組織球肉腫におけるMMPの免疫組織化学的検索 (2012)
  • Author(s): 船戸里紗，米丸加余子，村上麻美，森崇，丸尾幸嗣，柳井徳磨，酒井洋樹
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 盛岡
• [Presentation] 猫の腫瘍 (2011)
  • Author(s): 酒井洋樹
  • Organizer: 第32回動物臨床医学会年次大会
  • Place of Presentation: 大阪
• [Presentation] 犬の組織球肉腫におけるMacrophage scavenger receptor 1(CD204)の発現 (2011)
  • Author(s): 加藤祐樹,酒井洋樹, Nakagawa Tizianne,森崇,星野有希,丸尾幸嗣,村上麻美,柳井徳磨
  • Organizer: 第152回日本獣医学会学術集会
  • Place of Presentation: 大阪
• [Presentation] 犬血管肉腫細胞株の細胞増殖に対するphosphatidyl-inositol-3 kinase(PI3K)/Akt/mammalian target of rapamycin(mTOR)シグナル伝達経路の関与 (2011)
  • Author(s): 村井厚子,酒井洋樹,児玉篤史,星野有希,森崇,丸尾幸嗣,柳井徳磨
  • Organizer: 第152回日本獣医学会学術集会
  • Place of Presentation: 大阪
• [Presentation] イヌ血管腫瘍における血小板由来成長因子とその受容体の発現(Expression of platelet derived growth factor and its receptors in canine vascular tumors) (2011)
  • Author(s): Salem Abou Asa Samah,酒井洋樹,村井厚子,村上麻美,柳井徳磨
  • Organizer: 第151回日本獣医学会学術集会
• [Presentation] 血管系腫瘍におけるAkt/ mammalian target of rapamycin (mTOR) / eIF4E-binding protein 1
  • Author(s): 村井厚子、Salem Samah、平田暁大、酒井洋樹、柳井徳磨
  • Organizer: 第153回日本獣医学会学術集会
  • Place of Presentation: 大宮
• [Presentation] 犬血管肉腫細胞株の細胞増殖に対するphosphatidyl-inositol-3 kinase(PI3K)/Akt/mammalian target of rapamycin(mTOR)シグナル伝達経路の関与
  • Author(s): 村井厚子，酒井洋樹, 児玉篤史, 星野有希, 森崇, 丸尾幸嗣, 柳井徳磨
  • Organizer: 第152回日本獣医学会学術集会
  • Place of Presentation: 堺
• [Presentation] Expression of platelet derived growth factor and its receptors in canine vascular tumors
  • Author(s): Abou Asa Samah Salem、酒井洋樹、村井厚子、村上麻美、柳井徳磨
  • Organizer: 第151回日本獣医学会学術集会
  • Place of Presentation: 府中
• [Book] 犬と猫の臨床腫瘍学 (2013)
  • Author(s): 丸尾幸嗣,森崇,酒井洋樹
  • Total Pages: 265
  • Publisher: インターズー
• [Book] 犬と猫の臨床腫瘍学 (2013)
  • Author(s): 丸尾幸嗣，森 崇，酒井洋樹
  • Total Pages: 265
  • Publisher: インターズー