Monitoring of naturally feed contamination of zearalenone in cattle herds and its preventive measures

• Project/Area Number: 23580441
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: Kagoshima University
• Principal Investigator: TAKAGI Mitsuhiro 鹿児島大学, 獣医学部, 准教授 (40271746)
• Co-Investigator(Kenkyū-buntansha): UNO Seiichi 鹿児島大学, 水産学部, 准教授 (50381140) ; OTOI Takeshige 山口大学, 共同獣医学部, 教授 (30311814)
• Research Collaborator: JOHANNA Fink-Gremmels ユトレヒト大学, 獣医学部, 教授 ; 國師 恵美子 鹿児島大学, 大学院連合農学研究科 ; 中村 正幸 鹿児島大学, 農学部, 准教授 ; 宮本 明夫 帯広畜産大学, 畜産学研究科, 教授 ; 出口 栄三郎 鹿児島大学, 共同獣医学部, 教授 ; 川村 理 香川大学, 農学部, 教授 ; 志賀 慧 鹿児島大学, 農学部獣医学科 ; 松本 大策 (有)シェパード中央家畜診療所 ; 蓮沼 浩 鹿児島大学, 大学院連合獣医学研究科 ; 伏見 康生 鹿児島大学, 大学院連合獣医学研究科 ; 岡本 光司 鹿児島県曽於農業共済組合家畜診療所 ; 渡邊 麗 (新屋 麗) 鹿児島県曽於農業共済組合家畜診療所
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: ウシ / ブタ / ゼアラレノン / 尿 / マイコトキシン吸着剤 / 繁殖性 / 家畜 / 繁殖

Outline of Final Research Achievements

We investigated effects of a commercially available mycotoxin adsorbent (MA) using Zearalenone (ZEN) contaminated herd as a model herd. The results indicate that MA supplementation has beneficial effects in reducing ZEA absorption from the intestine of cattle, reversing hepatic effects. Additionally, our field trials revealed that 1) natural ZEN contamination resulting in urine levels below the threshold value does not affect embryo production in Japanese Black and Holstein cows undergoing superovulation, and 2) the monthly (seasonally) variation of urinary ZEN concentrations existing under the standard dairy farming condition. Moreover, the effects of in vitro exposure of porcine spermatozoa to ZEN were studied by evaluating several parameters of an in vitro fertilization (IVF) system. Overall, the results indicate that prolonged exposure of boar spermatozoa to ZEN up to 1000 μg/l under reduced metabolic conditions does not affect their in vitro function.

Research Products

• [Journal Article] Application of mycotoxin adsorbent to cattle feed naturally contaminated with zearalenone: Urinary zearalenone excretion and association with anti-Mullerian hormone. (2014)
  • Author(s): Fushimi Y, Takagi M, Hasunuma H, Uno S, Kokushi E, Watanabe U, Liu J, Marey MA, Miyamoto A, Otoi T, Deguchi E, Fink-Gremmels J.
  • Journal Title: World Mycotoxin Journal Volume: 7 Pages: 367-378
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Application of mycotoxin adsorbent to cattle feed naturally contaminated with zearalenone: Urinary zearalenone excretion and association with anti-Mullerian hormone (2014)
  • Author(s): Y. Fushimi, M. Takagi, H. Hasunuma, S. Uno, E. Kokushi, U. Watanabe, J. Liu, M. Marey, A. Miyamoto, T. Otoi, E. Deguchi, and J. Fink-Gremmels
  • Journal Title: World Mycotoxin Journal Volume: In press
  • Peer Reviewed
• [Journal Article] Relationship between urinary zearalenone concentration and embryo production in superovulated cattle (2013)
  • Author(s): M. Takagi, T. Hirai, S. Shiga, S. Uno, E. Kokushi, T. Otoi, E. Deguchi, C. Tshering and J. Fink- Gremmels
  • Journal Title: Archives Animal Breeding Volume: 36 Issue: 1 Pages: 360366-360366
  • DOI: 10.7482/0003-9438-56-036
  • Peer Reviewed
• [Journal Article] Periodic Alteration in Urinary Zearalenone Excretion in a Dairy Cattle Herd (2013)
  • Author(s): M. Takagi, S. Shiga, S. Uno, E. Kokushi, T. Otoi, C. Tshering, E. Deguchi and J. Fink-Gremmels
  • Journal Title: Animal Nutrition and Feed Technology Volume: 13 Pages: 303310-303310
  • Peer Reviewed
• [Journal Article] Effects of long-term in vitro exposure of ejaculated boar sperm to zearalenone and α-zearalenol in sperm liquid storage medium (2013)
  • Author(s): R. Sambuu, M. Takagi, Z. Namula, M. Nii, M. Taniguchi, S. Uno, E. Kokushi, C. Tshering, R. Rodrigues dos Santos, J. Fink-Gremmels and T. Otoi
  • Journal Title: Animal Science Journal Volume: 84 Issue: 1 Pages: 28-34
  • DOI: 10.1111/j.1740-0929.2012.01033.x
  • Peer Reviewed
• [Journal Article] Natural contamination of dietary rice straw with zearalenone and urinary zearalenone concentrations in a cattle herd (2012)
  • Author(s): H. Hasunuma, M. Takagi, O. Kawamura, C. Taniguchi, M. Nakamura, T. Chuma, S. Uno, E. Kokushi, D. Matsumoto, C. Tshering, E. Deguchi, and J. Fink-Gremmels
  • Journal Title: Journal of Animal Science Volume: 90 Pages: 1610-1616
  • Peer Reviewed
• [Journal Article] Natural contamination of dietary rice straw with zearalenone and urinary zearalenone concentrations in a cattle herd (2012)
  • Author(s): H. Hasunuma, M. Takagi, O. Kawamura, C. Taniguchi, M. Nakamura, T. Chuma, S. Uno, E. Kokushi, D. Matsumoto, C. Tshering, E. Deguchi, and J. Fink-Gremmels
  • Journal Title: J Anim Sci Volume: In press
  • Peer Reviewed
• [Journal Article] Effects of long-term in vitro exposure of ejaculated boar sperm to zearalenone and α-zearalenol in sperm liquid storage medium (2012)
  • Author(s): R. Sambuu, M. Takagi, Z. Namula, M. Nii, M. Taniguchi, S. Uno, E. Kokushi, C. Tshering, R. R. Santos, J. Fink-Gremmels and T. Otoi
  • Journal Title: Anim Sci J Volume: In press
  • Peer Reviewed
• [Journal Article] Effects of Exposure to Zearalenone on Porcine Oocytes and Sperm During Maturation and Fertilization In Vitro (2011)
  • Author(s): R. Sambuu, M. Takagi, Z. Namula, T. Otoi, S. Shiga, R. R. Santos and J. Fink-Gremmels
  • Journal Title: J Reprod Dev Volume: 57 Pages: 547-550
  • NAID: 10029593519
  • Peer Reviewed
• [Presentation] Application of mycotoxin adsorbent to cattle feed naturally contaminated with zearalenone: impact on ovarian function and serum anti-Mullerian hormone. (2014)
  • Author(s): Takagi M, Fushimi Y, Uno S, Kokushi E, Watanabe U, Liu J, Marey M, Miyamoto A, Otoi T, Deguchi E, Fink-Gremmels J
  • Organizer: 9 th International Ruminant Reproduction Symposium
  • Place of Presentation: ホテル日航ノースランド帯広（北海道帯広市）
  • Year and Date: 2014-08-25 – 2014-08-29
• [Presentation] Effects of a mycotoxin adsorbent applied with the diet to cattle being exposed to rice straw naturally contaminated with zearalenone (2012)
  • Author(s): M. Takagi,* H. Hasunuma, S. Uno, E. Kokushi, D. Matsumoto, U. Watanabe, K. Okamoto, C. Tshering, E. Deguchi, and J. Fink-Gremmels
  • Organizer: XXVII World Buiatrics Congress 2012（世界牛病学会）
  • Place of Presentation: リスボン（ポルトガル）
• [Presentation] Natural contamination of dietary rice straw with zearalenone and urinary zearalenone concentrations in a cattle herd (2012)
  • Author(s): H. Hasunuma, M. Takagi*, O. Kawamura, C. Taniguchi, M. Nakamura, T. Chuma,S. Uno, E. Kokushi, D. Matsumoto, C. Tshering, E. Deguchi,and J. Fink-Gremmels
  • Organizer: XXVII World Buiatrics Congress 2012（世界牛病学会）
  • Place of Presentation: リスボン（ポルトガル）
• [Presentation] 飼養環境下の牛群におけるカビ毒吸着剤の飼料添加効果 (2012)
  • Author(s): 蓮沼浩、髙木光博、宇野誠一、國師恵美子、松本大策、岡本光司、渡邊麗、出口栄三郎
  • Organizer: 平成２４年度日本産業動物獣医学会（九州地区）
  • Place of Presentation: 宮崎