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Trial of new virus isolation from industrial animals

Research Project

Project/Area Number 23580451
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Clinical veterinary science
Research InstitutionAzabu University

Principal Investigator

OCHIAI Hideharu  麻布大学, 付置研究所, 准教授 (20247307)

Co-Investigator(Kenkyū-buntansha) MIZUTANI Tetsuya  国立感染症研究所, ウイルスII部 (70281681)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsウイルス / 衛生 / 感染症 / ヤギ / エンテロウイルス / 次世代シーケンサー / 産業動物 / 迅速検出 / ブタ
Research Abstract

Goat diarrheal feces were subjected to metagenome analysis by the next-generation sequencing. Nucleotide sequences with homology to enteroviruses were obtained. Primers for RT-PCR were designed based on the nucleotide sequence of these sequences at the 5'-untranslated region, and we determined 563 bp nucleotide sequences that showed homology to bovine-like and ovine enteroviruses (77–87 %). We named the virus detected in this study goat enterovirus G1 (GEV-G1). In the phylogenetic analysis, GEV-G1 belonged to a cluster containing ovine enteroviruses. To our knowledge, this is the first report on nucleotide sequences of an enterovirus infecting Japanese goats.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (2 results)

All 2014

All Journal Article (2 results) (of which Peer Reviewed: 2 results)

  • [Journal Article] Detection of enterovirus genome sequence from diarrheal feces of goat2014

    • Author(s)
      Omatsu T, Tsuchiaka S, Hirata T, Shiroma Y, Okazaki S, Katayama Y, Oba M, Nishiura N, Sassa Y, Furuya T, Nagai M, Ochiai H, Tamaki S, Mizutani T.
    • Journal Title

      Virus Genes

      Volume: 48(3) Issue: 3 Pages: 550-2

    • DOI

      10.1007/s11262-014-1057-9

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Detection of enterovirus genome sequence from diarrheal feces of goat.2014

    • Author(s)
      T. Mizutani, Y. Sayama, A. Nakanishi, H. Ochiai, K. Sakai, K. Wakabayashi, K. Tanaka, E. Miura, I. Kurane, S. Morikawa, S. Ono
    • Journal Title

      Virus Genes

      Volume: Inpress

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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