Trial of new virus isolation from industrial animals

• Project/Area Number: 23580451
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: Azabu University
• Principal Investigator: OCHIAI Hideharu 麻布大学, 付置研究所, 准教授 (20247307)
• Co-Investigator(Kenkyū-buntansha): MIZUTANI Tetsuya 国立感染症研究所, ウイルスII部 (70281681)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: ウイルス / 衛生 / 感染症 / ヤギ / エンテロウイルス / 次世代シーケンサー / 産業動物 / 迅速検出 / ブタ

Research Abstract

Goat diarrheal feces were subjected to metagenome analysis by the next-generation sequencing. Nucleotide sequences with homology to enteroviruses were obtained. Primers for RT-PCR were designed based on the nucleotide sequence of these sequences at the 5'-untranslated region, and we determined 563 bp nucleotide sequences that showed homology to bovine-like and ovine enteroviruses (77–87 %). We named the virus detected in this study goat enterovirus G1 (GEV-G1). In the phylogenetic analysis, GEV-G1 belonged to a cluster containing ovine enteroviruses. To our knowledge, this is the first report on nucleotide sequences of an enterovirus infecting Japanese goats.

Research Products

• [Journal Article] Detection of enterovirus genome sequence from diarrheal feces of goat (2014)
  • Author(s): Omatsu T, Tsuchiaka S, Hirata T, Shiroma Y, Okazaki S, Katayama Y, Oba M, Nishiura N, Sassa Y, Furuya T, Nagai M, Ochiai H, Tamaki S, Mizutani T.
  • Journal Title: Virus Genes Volume: 48(3) Issue: 3 Pages: 550-2
  • DOI: 10.1007/s11262-014-1057-9
  • Peer Reviewed
• [Journal Article] Detection of enterovirus genome sequence from diarrheal feces of goat. (2014)
  • Author(s): T. Mizutani, Y. Sayama, A. Nakanishi, H. Ochiai, K. Sakai, K. Wakabayashi, K. Tanaka, E. Miura, I. Kurane, S. Morikawa, S. Ono
  • Journal Title: Virus Genes Volume: Inpress
  • Peer Reviewed