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Development of microbial factory for bioplastic production adapting inedible biomass hydrolysates

Research Project

Project/Area Number 23580452
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Boundary agriculture
Research InstitutionHokkaido University

Principal Investigator

OOI Toshihiko  北海道大学, 工学(系)研究科(研究院), 准教授 (40223713)

Co-Investigator(Kenkyū-buntansha) TAGUCHI Seiichi  北海道大学, 大学院工学研究院, 教授 (70216828)
KEN'ICHRO Matsumoto  北海道大学, 大学院工学研究院, 准教授 (80360642)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywordsバイオプラスチック / バイオポリエステル / 非可食バイオマス / 微生物工場 / 糖化 / 乳酸ポリマー / バイオマス / 酵素糖化 / バイオリファイナリー
Research Abstract

Lignocellulosic biomass is mainly composed of glucose and xylose as the major sugars. The use of glucose for the biopolymer production has been demonstrated to be efficient whereas, the use of xylose is inefficient. However efficient utilization of both sugars is essential. In this study, methods for the efficient utilization of xylose were developed and enhanced to obtain high productivities of biopolymers. For P(LA-co-3HB) production lactic acid supplementation into the cell contributed to the inprovement of polymer yield from xylose. In addition xylose was found to be superiorb to glucose in giving higher LA fraction in the copolymer. The uptake of xylose by GatC which is galactitol transporter also effective to produce the copolymer. The approach for high yield production of biopolymers in combination with the lignocellulosic-derived mix sugars have a potential for the establishment of biorifinary for the production of variety of biopolymers.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (22 results)

All 2014 2013 2012 2011 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (16 results) Remarks (2 results)

  • [Journal Article] Enhanced production of poly(lactate-co-3-hydroxybutyrate) from xylose in engineered Escherichia coli overexpressing a galactitol transporter2014

    • Author(s)
      Nduko JM, Matsumoto K, Ooi T, Taguchi S
    • Journal Title

      Appl Microbiol Biotechnol.

      Volume: 98 Issue: 6 Pages: 2453-2460

    • DOI

      10.1007/s00253-013-5401-0

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effectiveness of xylose utilization for high yield production of lactate-enriched P(lactate-co-3- hydroxybutyrate) using a lactate-overproducing strain of Escherichia coli and an evolved lactate-polymerizing enzyme2013

    • Author(s)
      JM. Nduko, K. Matsumoto, T. Ooi, S. Taguchi
    • Journal Title

      Metab. Eng

      Volume: vol.15 Pages: 159-166

    • DOI

      10.1016/j.ymben.2012.11.007

    • NAID

      120005228684

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effectiveness of xylose utilization for high yield production of lactate-enriched P(lactate-co-3-hydroxybutyrate) using a lactate-overproducing strain of Escherichia coli and an evolved lactate-polymerizing enzyme2013

    • Author(s)
      John Masani NDUKO, Ken’ichiro MATSUMOTO, Toshihiko OOI and Seiichi TAGUCH
    • Journal Title

      Metabolic Engineering

      Volume: 15 Pages: 159-166

    • NAID

      120005228684

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Polyhydroxyalkanoates production from cellulose hydrolysate in Escherichia coli LS5218 with superior resistance against 5-hydroxymethylfurfural2012

    • Author(s)
      J.M.Nduko, W.Suzuki, K.Matsumoto, H.Kobayashi, T.Ooi, A.Fukuoka, S.Taguchi
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 113 Issue: 1 Pages: 70-72

    • DOI

      10.1016/j.jbiosc.2011.08.021

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 植物バイオマス由来の酵素糖化物を炭素源としたバイオポリエステルの生産2014

    • Author(s)
      大井俊彦、佐々木勝平、松本謙一郎、田口精一
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      明治大学(川崎
    • Related Report
      2013 Final Research Report
  • [Presentation] 植物バイオマス由来の酵素糖化物を炭素源としたバイオポリエステルの生産2014

    • Author(s)
      大井俊彦、佐々木勝平、松本謙一郎、田口精一
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学 川崎市多摩区
    • Related Report
      2013 Annual Research Report
  • [Presentation] Efficient production of poly(lactate-co-3- hydroxybutyrate) using hemicellulose-derived sugar, xylose, in engineered Escherichia coli overexpressing a galactitol transporter2013

    • Author(s)
      JM. Nduko, K. Matsumoto, T. Ooi, S. Taguchi
    • Organizer
      Frontier Chemistry Center International Symposium 2013
    • Place of Presentation
      Hokkaido University (Sapporo)
    • Related Report
      2013 Final Research Report
  • [Presentation] 植物バイオマスを用いた共重合ポリエステルの生産2013

    • Author(s)
      木勝平、大井俊彦、ンドゥコジョン、松本謙一郎、田口精一
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      広島国際会議場(広島)
    • Related Report
      2013 Final Research Report
  • [Presentation] 非可食性植物バイオマスからのP(3HB)の微生物生産2013

    • Author(s)
      大井俊彦、佐々木勝平、ンドゥコジョン、松本謙一郎、田口精一
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      広島国際会議場(広島)
    • Related Report
      2013 Final Research Report
  • [Presentation] 植物バイオマスを用いた共重合ポリエステルの生産2013

    • Author(s)
      佐々木勝平、大井俊彦、ンドゥコジョン、松本謙一郎、田口精一
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      広島国際会議場 広島市中区
    • Related Report
      2013 Annual Research Report
  • [Presentation] Efficient production of poly(lactate-co-3-hydroxybutyrate) using hemicellulose-derived sugar, xylose, in engineered Escherichia coli overexpressing a galactitol transporter2013

    • Author(s)
      Nduko JM, Matsumoto K, Ooi T, Taguchi S
    • Organizer
      Frontier Chemistry Center International Symposium 2013
    • Place of Presentation
      北海道大学 札幌市北区
    • Related Report
      2013 Annual Research Report
  • [Presentation] Biosynthesis of polyhydroxyalkanoates from cellulose hydrolysate in Escherichia coli strain with superior tolerance to 5-hydroxymethylfurfural2012

    • Author(s)
      JM Nduko, K. Matsumoto, H. Kobayashi, T. Ooi, A. Fukuoka, S. Taguchi
    • Organizer
      The 5th GCOE International Symposium
    • Place of Presentation
      Hokkaido University (Sapporo)
    • Year and Date
      2012-02-22
    • Related Report
      2013 Final Research Report
  • [Presentation] Efficient bioconversion of lignocellulosic biomass-derived sugars into poly(lactate-co-3- hydroxybutyrate) by metabolically engineered Escherichia coli2012

    • Author(s)
      JM. Nduko, K. Matsumoto, T. Ooi, S. Taguchi
    • Organizer
      15th International Biotechnology Symposium and Exhibition
    • Place of Presentation
      EXICO, Daegu (Korea)
    • Related Report
      2013 Final Research Report
  • [Presentation] Lactate-based polyester production by recombinant bacteria using lignocellulosic biomass sugars as carbon sources2012

    • Author(s)
      JM. Nduko, K. Matsumoto, T. Ooi, S. Taguchi
    • Organizer
      Japan-China-Korea Joint Symposium on Enzyme Engineering
    • Place of Presentation
      Kanazawa Excel Hotel (Kanazawa)
    • Related Report
      2013 Final Research Report
  • [Presentation] Lactate-based polyesters production by recombinant bacteria using lignocellulosic biomass sugars as carbon sources2012

    • Author(s)
      John Masani Nduko,Ken'ichiro Matsumoto, Toshihiko Ooi, Seiichi Taguchi
    • Organizer
      Japan-Chica-Korea Joint Symposium on Enzyme Engineering
    • Place of Presentation
      Kanazawa Exel Hotel, kanazawa, Japan
    • Related Report
      2012 Research-status Report
  • [Presentation] Efficient microbial production of (R)-3-hydroxybutyric acid by engineered Escherichia coli.2012

    • Author(s)
      Toshihiko Ooi, Takehiro Okei, Inori Honma, Tsuyoshi Watanabe, Ken'ichiro Matsumoto, Seiichi Taguchi
    • Organizer
      15th International Biotechnology Symposium
    • Place of Presentation
      EXICO,Daegu, Korea
    • Related Report
      2012 Research-status Report
  • [Presentation] Biosynthesis of polyhydroxyalkanoates from cellulose hydrolysate in Escherichia coli strain with superior tolerance to 5-hydroxymethylfurfural2012

    • Author(s)
      John Masani Nduko, Ken’ichiro Matsumoto, Hirokazu Kobayashi, Toshihiko Ooi, Atsushi Fukuoka, Seiichi Taguchi
    • Organizer
      The 5th GCOE International Symposium
    • Place of Presentation
      北海道大学(札幌市)
    • Related Report
      2011 Research-status Report
  • [Presentation] Polyhydroxyalkanoates production from cellulose hydrolysate in Escherichia coli LS5218 with superior resistance against 5-hydroxymethylfulfural2011

    • Author(s)
      JM Nduko, W. Suzuki, K. Matsumoto, H. Kobayashi, T. Ooi, A. Fukuoka, S. Taguchi
    • Organizer
      2011 Taiwan-Japan Bilateral Polymer Symposium
    • Place of Presentation
      National China University (Taiwan)
    • Year and Date
      2011-09-15
    • Related Report
      2013 Final Research Report
  • [Presentation] Production of lactate-based polyesters from xylose in Escherichia coli2011

    • Author(s)
      JM Nduko, K. Matsumoto, T. Ooi, S. Taguchi
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      東京農工大学(東京)
    • Related Report
      2013 Final Research Report
  • [Presentation] Polyhydroxyalkanoates Production from Cellulose Hydrolysate in Escherichia coli LS5218 with Superior Resistance to 5-Hydroxymethylfurfural2011

    • Author(s)
      John Masani Nduko, Wakako Suzuki, Ken’ichiro Matsumoto, Hirokazu Kobayashi, Toshihiko Ooi, Atsushi Fukuoka, Seiichi Taguchi:
    • Organizer
      2011 Taiwan-Japan Bilateral Polymer Symposium
    • Place of Presentation
      National Chiao University (Taiwan)
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.eng.hokudai.ac.jp/labo/seika/TOP.html

    • Related Report
      2013 Final Research Report
  • [Remarks] バイオ分子工学研究室ウェブページ

    • URL

      http://www.eng.hokudai.ac.jp/labo/seika/TOP.html

    • Related Report
      2012 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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