Study on overwintering mechanism of dormant buds of trees for sustainable production of woody biomass
| Project/Area Number |
23580453
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Boundary agriculture
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| Research Institution | Hokkaido University |
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Principal Investigator |
ARAKAWA Keita 北海道大学, (連合)農学研究科(研究院), 准教授 (00241381)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 冬芽 / 越冬機構 / 耐寒性 / 休眠 / カラマツ冬芽 / 器官外凍結 / 過冷却 / 凍結抵抗性 |
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| Research Abstract |
In this study, extraorgan-freezing and endodormancy release of larch dormant buds were studied for understanding overwintering mechanism of dormant buds of trees.
Cryo-scanning electron microscopic study revealed that primordial cells were partially dehydrated at subzero temperatures and water remaining in primordial cells was kept in the deep supercooling state. In this tissue, accumulation of several carbohydrates such as pinitol and sucrose were detected as major components. However, it is difficult to explain that high freezing tolerance by deep supercooling capability of primordial tissue is determined by accumulation of major carbohydrates alone. Possible contribution of minor components such as supercooling-facilitating substances might be studied in larch dormant buds. In addition, proteomic analysis by using LC-MS/MS showed that only minor changes in soluble protein composition occurred in larch dormant buds within three weeks during endodormancy release.
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Report
(4 results)
Research Products
(40 results)
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[Journal Article] Analysis of supercooling activity of tannin-related polyphenols2013
Author(s)
Kuwabara,C.,Wang,D.,Endoh,K.,Fukushi,Y.,Arakawa,K.,Fujikawa,S.
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Journal Title
Cryobiology
Volume: 67
Issue: 1
Pages: 40-49
DOI
Related Report
Peer Reviewed
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[Journal Article] Evaluation on efficacy of supercooling-facilitating substances by various measurements2012
Author(s)
Kuwabara, C., Wang, D., Kasuga, J., Moriwaka, G., Arakawa, K., Koyama, T., Inada, T., Fujikawa, S.
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Journal Title
Cryobiology and Cryotechnology
Volume: 58
Pages: 99-103
NAID
Related Report
Peer Reviewed
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[Presentation] Supercooling-facilitating hydrolyzable tannins isolated from xylem tissues of Cercidiphyllum japonicum2012
Author(s)
Wang,D.,Kuwabara,C.,Endo,K.,Fukushi,Y.,Fujikawa,S.,Arakawa,K.
Organizer
Plant and Microbe Adaptations to Cold
Place of Presentation
Hokkaido University,Sapporo
Related Report
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