| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Outline of Final Research Achievements |
To develop an effective useful protein expression system by edible basidiomycetous fungi, improvements of Lentinula edodes transfomation method and laccase gene expression in L. edodes were studied. As a result, we were able to simplify the protoplast preparation method. Further, as a result of L. edodes transformation by laccase gene expression vector pChG-clcc1, a transformant exhibited more than two times higher laccase activity compared to the host was obtained. In addition, we isolated a laccase gene of lacquer (Toxicodendron vernicifluum) as a heterologous laccase gene for expression by L. edodes, and sequenced the full length of the laccase cDNA.
|
