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Detection and quantity of vascular bundles in oil palm trunk using cell wall birefringence on exposure to polarized light

Research Project

Project/Area Number 23580463
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Boundary agriculture
Research InstitutionJapan International Research Center for Agricultural Sciences

Principal Investigator

MURATA Yoshinori  独立行政法人国際農林水産業研究センター, その他部局等, その他 (40322664)

Co-Investigator(Kenkyū-buntansha) KOSUGI Akihiko  (独)国際農林水産業研究センター, 生物資源・利用領域, プロジェクトリーダー (70425544)
安部 久  独立行政法人森林総合研究所, その他部局等, 研究員 (80343812)
Co-Investigator(Renkei-kenkyūsha) ABE Hisashi  (独)森林総合研究所, 木材特性領域, 主任研究員 (80343812)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsオイルパーム / 維管束組織 / 柔組織 / 偏光 / 複屈折 / αセルロース / 維管束 / トランク(幹) / セルロース / X線回析 / 近赤外線 / 維管束(VB) / 柔組織(PT) / トランク(幹) / Elaeis guineensis / オイルパームトランク
Research Abstract

Vascular bundles (VBs) of oil palm trunks exhibited birefringence due to polarized orientation of their cell wall components, although another component of the trunks, parenchyma tissue (PT), did not. The intensity of the birefringence depended on the VB composition; therefore, it was possible to estimate the VB content readily using a polarizing microscope connected to an image analyzer. The method proposed in this manuscript should allow easier quantification of VBs in oil palm trunks than methods using X-ray diffraction. Both VBs and PT contained cellulose, hemicelluloses, lignin, and starch; however, the cellulose content of PT was less than half that of VBs. This difference in cellulose content between VB and PT may explain why VBs were detected under polarizing light.

Report

(5 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Products Report
  • Research Products

    (9 results)

All 2015 2013 2012 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (2 results)

  • [Journal Article] Detection of vascular bundles using cell wall birefringence on exposure to polarized light2015

    • Author(s)
      Yoshinori Murata^1*, Satoshi Kubo^2, Eiji Togawa^2, Sitti Fatimah Binti Mhd Ramle^3, Othman Sulaiman^3, Rokiah Hashim^3, Wan Asma Ibrahim^4, Akihiko Kosugi^1, Akiko Hirooka^1, and Hisashi Abe^2
    • Journal Title

      Industrial Crops and Products

      Volume: 65 Pages: 190-197

    • DOI

      10.1016/j.indcrop.2014.11.055

    • Related Report
      Products Report
    • Peer Reviewed
  • [Journal Article] Estimation of the Ratio of Vascular Bundles to Parenchyma Tissue in Oil Palm Trunks using NIR Spectroscopy2013

    • Author(s)
      Abe H, Murata Y, (他5名)
    • Journal Title

      Bioresources

      Volume: 8(2) Pages: 1573-1581

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of sap squeezing systems from oil palm trunk2013

    • Author(s)
      Murata Y, Tanaka R (他10名)
    • Journal Title

      Biomass and Bioenergy

      Volume: 51 Pages: 8-16

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Estimation of The ratio of vascular bundles to parenchyma tissue in oil palm trunks using NIR spectroscopy.2013

    • Author(s)
      Abe, H., M. Yoshinori, K. Satoshi, K. Watanabe, R. Tanaka, et al.,
    • Journal Title

      BioResouces

      Volume: 8(2), Pages: 1573-1581

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Development of sap compressing systems from oil palm trunk.2013

    • Author(s)
      Murata, Y., R. Tanaka, K. Fujimoto, A. Kosug, T. Arai, et al.,
    • Journal Title

      Biomass and Bioenergy

      Volume: 51, 8-16. Pages: 8-16

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Efficient saccharification of ammonia soaked rice straw by combination of Clostridium thermocellum cellulosome and Thermoanaerobacter brockiiβ-glucosidase2012

    • Author(s)
      Waeonukul R, Kosugi A (Corresponding author、他7名)
    • Journal Title

      BioresourTechnol

      Volume: 107 Pages: 352-7

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Efficient ethanol production from separated parenchyma and vascular bundle of oil palm trunk.2012

    • Author(s)
      Prawitwong, P., A. Kosugi, T. Arai, L. Deng, K.C. Lee, D. et al.,
    • Journal Title

      Bioresour Technol

      Volume: 125, Pages: 37-42

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Detection of vascular bundle in oil palm trunk by polarization microscopy2012

    • Author(s)
      Yoshinori Murata, Satoshi Kubo, Akihiko Kosugi, Yutaka Mori, Hisashi Abe
    • Organizer
      日本農芸化学会 2012年度大会(京都)
    • Place of Presentation
      京都女子大学
    • Related Report
      2011 Research-status Report
  • [Presentation] Detection of vascular bundle in oil palm trunk by polarization microscopy

    • Author(s)
      Yoshinori Murata, et al.
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都、京都女子大
    • Related Report
      2013 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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