| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Research Abstract |
Seed formation is a survival strategy of higher plants that protect the juvenile embryo from unfavorable conditions and facilitates the dispersion of offspring into a new habitat. The chromatin structures play important roles in a variety of nuclear functions by influencing the assembly of nuclear factors through epigenetic modifications. In the pioneer works of plant seed science, the relationships between structure and function of seed nuclei have been well documented, and the microscopic analyses have confirmed morphological changes of chromatin structures between the quiescent and germinated embryos, including the highly condensed state of chromatin structures. These observations demonstrate that the protective mechanisms of genome integrity must be mobilized in the plant seeds. In this study, we have purified the chromatin components from Arabidopsis dry seeds and conducted the subproteome analyzes by the use of 2D-PAGE in conjugation with mass spectrometric technology.
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