| Project/Area Number |
23580470
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Ibaraki University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAKUTA Yoshimitsu 九州大学, 大学院・農学研究院, 准教授 (00314360)
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| Co-Investigator(Renkei-kenkyūsha) |
YOSHIDA Shigeto 金沢大学, 医薬保健研究域, 教授 (10296121)
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| Research Collaborator |
YONEKURA Masami 茨城大学, 農学部, 教授 (40134168)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | レクチン / 溶血 / マラリア |
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| Research Abstract |
In this study, mutants of hemolytic lectin CEL-III were constructed to improve the hemolytic activity and change the Ca-dependency. A fusion protein of earthworm lectin with pore-forming domain of CEL-III was constructed and showed hemagglutinating activity with no Ca-dependency. Furthermore, amino acid residues involved in stacking interaction with carbohydrate in five carbohydrate binding sites of CEL-III were replaced to other amino acids. Among the CEL-III mutants, five mutants (H36F, Y134H, Y181F,Y181W, Y222F) have a stronger hemolytic activity than wild type CEL-III.
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