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Solubilization-inducing tag for solubilization, development of higher function and medicine for proteins.

Research Project

Project/Area Number 23580475
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied molecular and cellular biology
Research InstitutionKagoshima University

Principal Investigator

TOKUNAGA MASAO  鹿児島大学, 農学部, 教授 (20112782)

Co-Investigator(Kenkyū-buntansha) ISHIBASHI Matsujiro  鹿児島大学, 農学部, 准教授 (20305163)
TOKUNAGA Hiroko  鹿児島大学, 農学部, 技能補佐員 (60381191)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Keywords好塩性蛋白質 / 可溶化 / 高次構造形成 / まき戻り / 凝集耐性 / タグ / 可溶性 / 変性ストレス耐性 / Brevibacillus / scFv / 可溶性タグ / 金属結合タンパク質 / 好塩性酵素 / 好塩性微生物 / 融合蛋白質
Research Abstract

Halophilic proteins, acidic proteins with high content of negative charge, can function under high salt conditions without salting-out effects of salts. High solubility and negative charge repulsion make halophilic protein resistant to various stresses which induce denaturaion of protein, and thus, holophilic proteins are highly resistant to denaturation (resistant to irreversible-aggregation). We isolated several genes of halophilic proteins, characterized, and developed tag to construct fusion proteins.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (12 results)

All 2013 2012 2011 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (5 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Secretory production of single-chain antibody (scFv) in Brevibacillus choshinensis using novel fusion partner2013

    • Author(s)
      Tokunaga M, Mizukami M, Yamasaki K, Tokunaga H, Onishi H, Hanagata H, Ishibashi M, Miyauchi A, Tsumoto K, Arakawa T.
    • Journal Title

      Appl Microbiol Biotechnol

      Volume: 97(19) Pages: 8569-8580

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Amyloid fibril formation in vitro from halophilic metal binding protein : Its high solubility and reversibility minimized formation of amorphous protein aggregations2013

    • Author(s)
      Tokunaga Y, Matsumoto M, Tokunaga M, Arakawa T, Sugimoto Y.
    • Journal Title

      Protein Sci

      Volume: 22(11) Pages: 1582-1591

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Secretory production of single-chain antibody (scFv) in Brevibacillus choshinensis using novel fusion partner.2013

    • Author(s)
      Tokunaga M, Mizukami M, Yamasaki K, Tokunaga H, Onishi H, Hanagata H, Ishibashi M, Miyauchi A, Tsumoto K, Arakawa T.
    • Journal Title

      Applied Microbiology and Biotechnology

      Volume: 97 Issue: 19 Pages: 8569-8580

    • DOI

      10.1007/s00253-013-4695-2

    • Related Report
      2013 Annual Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Halophilic characterization of starch-binding domain from Kocuria variansα-amylase2012

    • Author(s)
      Yamaguchi R, Inoue Y, Tokunaga H, Ishibashi M, Arakawa T, Sumitani J, Kawaguchi T, Tokunaga M.
    • Journal Title

      Int J Biol Macromol

      Volume: 50(1) Pages: 95-102

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Halophilic characterization of starch-binding domain from Kocuria varians alpha-amylase2012

    • Author(s)
      R. Yamaguchi, I. Inoue, H. Tokunaga, M. Ishibashi, T. Arakawa, J. Sumitani, T. Kawaguchi, M. Tokunaga
    • Journal Title

      International Journal of Biological Macromolecules

      Volume: 50 Issue: 1 Pages: 95-102

    • DOI

      10.1016/j.ijbiomac.2011.10.007

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Brevibacillus発現系を用いた一本鎖抗体(scFv)の発現生産2012

    • Author(s)
      徳永正雄、山崎晃路、水上誠、大西廣優、徳永廣子、石橋松二郎、花方寛、宮内明、荒川力
    • Organizer
      生化学会2012年度大会
    • Place of Presentation
      福岡県福岡市
    • Related Report
      2013 Final Research Report
  • [Presentation] Brevibacillus発現系を用いた一本鎖抗体 (scFv) の発現生産2012

    • Author(s)
      徳永正雄、山崎晃路、水上 誠、大西廣優、徳永廣子、石橋松二郎、花方 寛、宮内 明、荒川 力
    • Organizer
      2012年度 日本生化学会大会
    • Place of Presentation
      福岡市
    • Related Report
      2012 Research-status Report
  • [Presentation] 塩依存的に巻き戻る中度好塩菌Kocuria varians由来α-アミラーゼの性質検討とクローニング2011

    • Author(s)
      山口類、石橋松二郎、徳永廣子、荒川力、徳永正雄
    • Organizer
      日第11回日本蛋白科学会年会
    • Place of Presentation
      大阪府吹田市
    • Related Report
      2013 Final Research Report
  • [Presentation] 中度好塩菌由来金属結合蛋白質の金属親和性と塩の効果

    • Author(s)
      尾辻大樹、西元愛里、石橋松二郎、徳永廣子、徳永正雄
    • Organizer
      日本生化学会 九州支部大会
    • Place of Presentation
      佐賀大学 農学部
    • Related Report
      2013 Annual Research Report
  • [Presentation] 塩依存的にまき戻る中度好塩菌Kocuria varians由来αーアミラーゼの性質検討とクローニング

    • Author(s)
      山口類、石橋松二郎、徳永廣子、荒川力、徳永正雄
    • Organizer
      第11回日本蛋白質科学会年会
    • Place of Presentation
      ホテル阪急エキスポパーク  大阪府
    • Related Report
      2011 Research-status Report
  • [Book] Halophilic Properties and their Manipulation and Application. in Extremophiles : Sustainable Resorces and Biotechnological Implications, First2013

    • Author(s)
      Arakawa T. Tokunaga H. Ishibashi M. Tokunaga M.
    • Publisher
      Edited by Om V. Singh (Wiley-Blackwell)
    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://ace1.agri.kagoshima-u.ac.jp/agri0029/information/2014/03/oubi-home.html

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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