Development of techniques for establishing the fully human antibody high producing human cell lines
| Project/Area Number |
23580477
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Kitakyushu National College of Technology |
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Principal Investigator |
INOUE Yuichi 北九州工業高等専門学校, 物質化学工学科, 准教授 (20284911)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAHARA Hiroharu 北九州工業高等専門学校, 物質化学工学科, 教授 (20321515)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Keywords | ヒト抗体 / ヒト細胞株 / ハイブリドーマ / レチノイド |
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| Research Abstract |
To produce fully human antibodies, cell fusion were examined using human cells. As a result, it was found that the cell fusion using the T lymphoid fusion partner such as A4H12 could generate universally human hybridomas that formed cell aggregation and increased their antibody production by retinoic acid. For the cell fusion, the quality of fetal bovine serum was the most important factor.
In addition, the production of recombinant fully human antibodies was also challenged. The introduction of the fructose transporter GLUT5 and antibody co-expression system into the human cell line SC-01MFP made it possible to produce them in the fructose-based medium up to about two-fold of the conventional glucose-based medium.
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Report
(4 results)
Research Products
(7 results)
