| Project/Area Number |
23590051
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
HONDA Akira 東京医科大学, 医学部, 教授 (10468639)
|
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| Co-Investigator(Kenkyū-buntansha) |
MIYAZAKI Teruo 東京医科大学, 医学部, 講師 (60532687)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMASHITA Kouwa 東北薬科大学, 薬学部, 教授 (80382670)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | ステロイド / LC-MS/MS |
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| Research Abstract |
We have developed a highly sensitive and specific method for the analysis of steroid profiles in biological samples including pathological tissue specimens. After the addition of deuterium-labeled internal standards, biological samples were saponified with 1N ethanolic KOH, extracted with n-hexane followed by n-hexane:ethylacetate (1:1), and derivatized into picolinyl esters before analysis by high performance liquid chromatography-positive electrospray ionization-tandem mass spectrometry (HPLC-PESI-MS/MS). Cholesterol related sterols, oxysterols, adrenal steroids and neurosteroids were quantified simultaneously. The effects of formaldehyde fixation and Mayer's hematoxylin staining on the steroid analysis of pathological tissue specimen were examined. These procedures did not affect significantly the results of the present analysis.
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