| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
In this study, we demonstrated that the coiled-coil domains isolated from the yeast cargo receptors Emp46p/47p show pH-dependent conversion of heteromeric to homomeric complexes, and identified the amino acid residues responsible for the regulation of the conversion. Mutations introduced at these residues altered the pH-dependency of the formation of heteromeric complexes. Moreover, using fluorescent protein fusions, the assembly of heteromeric complexes was successfully detected by FRET, which enabled real-time monitoring of the heteromeric complex formation. Thus, we have succeeded in providing strategies for application of the coiled-coil domains of Emp46p/47p to various pH-responsive bio-devices.
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