| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Rho family GTPase-specific guanine nucleotide exchange factors of Dbl family regulate a variety of cellular events including cytoskeletal arrangement, signal transduction, and gene expression through activation of Rho family GTPases. FLJ00018/CLG/PLEKHG2 is a guanine nucleotide exchange factor (GEF) for Rho family of small GTPases. In this research project, we have examined the regulation mechanisms of PLEKHG2 by phosphorylation and protein-protein interactions. We showed here that PLEKHG2 interacted with cytosolic actin or phosphoinositide containing PtdIns(4,5)P2, PtdIns(3,4,5)P3 and phosphatidic acid. We showed that PLEKHG2 was phosphorylated by tyrosine kinase Src or EGF receptor signals.
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