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Regulatory mechanism of expression of ETS transcription factor Myeloid elf-1-like factor (MEF) by tumor suppressors

Research Project

Project/Area Number 23590082
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biological pharmacy
Research InstitutionKumamoto University

Principal Investigator

SUICO Mary Ann Soten  熊本大学, 大学院生命科学研究部(薬), 助教 (20363525)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords転写因子 / がん抑制遺伝子 / MEF / 低酸素 / HIF1 / ETS転写因子 / 癌抑制遺伝子 / 翻訳後発現調節 / p53 / MDM2 / Rb / 翻訳後発現制御
Research Abstract

Myeloid elf-1-like factor (MEF) functions as transcriptional factor and plays critical roles in development, cellular differentiation, proliferation, transformation and immune responses. In the present study, we aim to determine the regulatory mechanisms of MEF expression by several cellular factors. First, we demonstrated that tumor suppressor p53 decreases the level of MEF protein through the induction of transcription of MDM2, an E3 ubiquitin ligase. Moreover, the findings also revealed that another tumor suppressor Rb increases the MEF protein level possibly by stabilizing its protein expression. Finally, our data further showed that hypoxia increases both the mRNA and protein levels of MEF through HIF1alpha, the main effector molecule of hypoxia. On the whole, the findings revealed in these studies provide a better understanding of how the transcriptional regulator MEF is influenced by tumor suppressors such as p53 and Rb, and by hypoxia-related protein HIF1alpha.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (40 results)

All 2014 2013 2012 2011 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (26 results) Remarks (4 results)

  • [Journal Article] Mild electrical stimulation at 0.1-ms pulse width induces p53 protein phosphorylation and G2 arrest in human epithelial cells.2013

    • Author(s)
      Fukuda ft, Suico MA, Koyama K, Omachi K, Kai Y, Matsuyama S, Mitsutake K, Taura M, Mo rino-Koga S, Shuto T, Kai H.
    • Journal Title

      Journal of Biological Chemistry

      Volume: 288(22) Issue: 22 Pages: 16117-16126

    • DOI

      10.1074/jbc.m112.442442

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rb/E2F1 regulates the innate immune receptor Toll-like receptor 3 in epithelial cells2012

    • Author(s)
      Taura M., et.al.
    • Journal Title

      Molecular and Cellular Biology

      Volume: 32(8) Issue: 8 Pages: 1581-1590

    • DOI

      10.1128/mcb.06454-11

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] STT3B-dependent posttranslational N-glycosylation as a surveillance system for secretory protein.2012

    • Author(s)
      Sato T., et.al.
    • Journal Title

      Molecular Cell

      Volume: 47(1) Issue: 1 Pages: 99-110

    • DOI

      10.1016/j.molcel.2012.04.015

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mild electrical stimulation and heat shock ameliorates progressive proteinuria and renal inflammation in mouse model of Alport syndrome.2012

    • Author(s)
      Tomoaki Koga
    • Journal Title

      PLoS One

      Volume: 7 Issue: 8 Pages: 43852-43852

    • DOI

      10.1371/journal.pone.0043852

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Diethyldithiocarbamate induces apoptosis in HHV-8-infected primary effusion lymphoma cells via inhibition of the NF-κB pathway.2012

    • Author(s)
      Matsuno T, Kariya R, Yano S, Morino-Koga S, Taura M, Suico MA, Shimauchi Y, Matsuyama S, Okamoto Y, Shuto T, Kai H, Okada S.
    • Journal Title

      Int J Oncol.

      Volume: 40(4) Pages: 1071-1078

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Synergism Between Interleukin (IL)-17 and Toll-like Receptor 2 and 4 Signals to Induce IL-8 Expression in Cystic Fibrosis Airway Epithelial Cells.2012

    • Author(s)
      Mizunoe S, Shuto T, Suzuki S, Matsumoto C, Watanabe K, Ueno-Shuto K, Suico MA, Onuki K, Gruenert DC, Kai H.
    • Journal Title

      J Pharmacol Sci.

      Volume: -

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Hyperthermia With Mild Electrical Stimulation Protects Pancreatic β-Cells From Cell Stresses and Apoptosis.2012

    • Author(s)
      Kondo T, Sasaki K, Matsuyama R, Morino-Koga S, Adachi H, Suico MA, Kawashima J, Motoshima H, Furukawa N, Kai H, Araki E.
    • Journal Title

      Diabetes.

      Volume: 61(4) Pages: 838-847

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Rb/E2F1 Regulates the Innate Immune Receptor Toll-Like Receptor 3 in Epithelial Cells.2012

    • Author(s)
      Taura M, Suico MA, Koyama K, Komatsu K, Miyakita R, Matsumoto C, Kudo E, Kariya R, Goto H, Kitajima S, Takahashi C, Shuto T, Nakao M, Okada S, Kai H.
    • Journal Title

      Mol Cell Biol.

      Volume: 32(8) Pages: 1581-1590

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] MEF/ELF4 transactivation by E2F1 is inhibited by p532011

    • Author(s)
      Taura M, Suico MA, Fukuda R, Koga T, Shuto T, Sato T, Morino-Koga S, Okada S, Kai H
    • Journal Title

      Nucleic Acids Res

      Volume: 39 Issue: 1 Pages: 76-88

    • DOI

      10.1093/nar/gkq762

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Glucose Uptake in Rat Skeletal Muscle L6 Cells Is Increased by Low-Intensity Electrical Current Through the Activation of the Phosphatidylinositol-3-OH kinase (PI-3K) / Akt Pathway.2011

    • Author(s)
      Yano S, Morino-Koga S, Kondo T, Suico MA, Koga T, Shimauchi Y, Matsuyama S, Shuto T, Sato T, Araki E, Kai H.
    • Journal Title

      J Pharmacol Sci.

      Volume: 115(1) Pages: 94-98

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Functional interplay of p53 and TLR links immune response and cancer2014

    • Author(s)
      Mary Ann SUICO
    • Organizer
      日本薬学会第134年会
    • Place of Presentation
      熊本
    • Related Report
      2013 Final Research Report
  • [Presentation] Specific pulse-width mild electrical stimulation induces p53 activation via p38 MAPK and ameliorates LPS-induced inflammatory cytokine response2013

    • Author(s)
      K. Omachi
    • Organizer
      15th International congress of immunology
    • Place of Presentation
      Milano, ITALY
    • Related Report
      2013 Final Research Report
  • [Presentation] p53 regulates the Ets trascription factor MEF/Elf4 via MDM22013

    • Author(s)
      スイコ メリーアン
    • Organizer
      第2回次世代創薬研究者養成塾
    • Place of Presentation
      熊本大学薬学部(熊本市中央区)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Specific pulse-width mild electrical stimulation induces p53 activation via p38 MAPK and ameliorates LPS-induced inflammatory cytokine response2013

    • Author(s)
      R. Fukuda, K. Omachi, M. A. Suico, K. Koyama, Y. Kai, S. Matsuyama, K. Mitsutake, S. Morino-Koga, T. Shuto, H. Kai
    • Organizer
      15th International congress of immunology
    • Place of Presentation
      Mico Milano Congressi, Milano, ITALY
    • Related Report
      2013 Annual Research Report
  • [Presentation] Crucial role of basal p53 in attenuation of inflammation and fibrosis in hereditary glomerular nephritis, Alport syndrome2013

    • Author(s)
      R. Fukuda, K. Koyama, T. Koga, Y. Kai, K. Omachi, K. Motomura, M. A. Suico, T. Shuto, H. Kai
    • Organizer
      15th International congress of immunology
    • Place of Presentation
      Mico Milano Congressi, Milano, ITALY
    • Related Report
      2013 Annual Research Report
  • [Presentation] 慢性腎疾患 Alport syndrome モデルマウスを用いた癌抑制遺伝子p53の腎保護的機能の解明2013

    • Author(s)
      福田 亮介
    • Organizer
      第86回日本薬理学会年会
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Related Report
      2012 Research-status Report
  • [Presentation] p53 regulates the Ets transcription factor MEF/Elf4 via MDM22012

    • Author(s)
      Mary Ann SUICO
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      北海道
    • Related Report
      2013 Final Research Report
  • [Presentation] p53 regulates the Ets transcription factor MEF/Elf4 via MDM22012

    • Author(s)
      Mary Ann SUICO
    • Organizer
      ASCB 2012 Annual Meeting
    • Place of Presentation
      San Francisco, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] 慢性腎疾患Alport syndromeモデルマウスを用いた癌抑制遺伝子p53の腎保護的機能の解明2012

    • Author(s)
      福田 亮介
    • Organizer
      第65回日本薬理学会西南部会
    • Place of Presentation
      熊本大学(熊本県)
    • Related Report
      2012 Research-status Report
  • [Presentation] p53 regulates the Ets transcription factor MEF/Elf4 via MDM2012

    • Author(s)
      Mary Ann Suico
    • Organizer
      第71回日本癌学会学術総会-がん研究とライフサイエンスの更なるリエゾン-
    • Place of Presentation
      ロイトン札幌(北海道)
    • Related Report
      2012 Research-status Report
  • [Presentation] 慢性腎疾患Alport syndromeにおける癌抑制遺伝子p53の腎保護的機能の解明2012

    • Author(s)
      福田 亮介
    • Organizer
      第11回次世代を担う若手ファーマ・バイオフォーラム
    • Place of Presentation
      九州大学(福岡県)
    • Related Report
      2012 Research-status Report
  • [Presentation] Specified pulse-width mild electrical stimulation induces p53 phosphorylation and transcriptional activation2012

    • Author(s)
      Ryosuke Fukuda
    • Organizer
      ICHO&JCTM 2012
    • Place of Presentation
      ハイアットリージェンシー京都(京都府)
    • Related Report
      2012 Research-status Report
  • [Presentation] p53 regulates the Ets transcription factor MEF/Elf4 via MDM22012

    • Author(s)
      M. Suico
    • Organizer
      ASCB 2012 Annual Meeting
    • Place of Presentation
      San Francisco, CA, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] Mef/Elf4 transactivation by E2F1 is inhibited by p532011

    • Author(s)
      Mary Ann SUICO
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Related Report
      2013 Final Research Report
  • [Presentation] The tumor suppressor Retinoblastoma protein positively regulates innate immune receptor Toll-like receptor 3 in epithelial cells2011

    • Author(s)
      K. Koyama
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      神奈川
    • Related Report
      2013 Final Research Report
  • [Presentation] Protective effects of p53 in a mouse model of progressive hereditary kidney disease Alport syndrome2011

    • Author(s)
      R. Fukuda
    • Organizer
      ASCB 51st Annual Meeting
    • Place of Presentation
      Denver, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Mef/Elf4 transactivation by E2F1 is inhibited by p53.2011

    • Author(s)
      M. Suico, M. Taura, R. Fukuda, T. Koga, T. Shuto, T. Sato, S. Morino, S. Okada, H. Kai
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] ストレス耐性付与および脂肪蓄積抑制作用を有する新たなphysical medicine2011

    • Author(s)
      松山 真吾、矢野 脩一郎、古賀(森野) 佐緒里、島内 裕一郎、岡本 友加、四反田 伊穂李、メリーアン スイコ、首藤 剛、甲斐 広文
    • Organizer
      第28回日本薬学会九州支部大会
    • Place of Presentation
      福岡大学薬学部(福岡)
    • Related Report
      2011 Research-status Report
  • [Presentation] 遺伝性腎疾患Alport症候群における癌抑制遺伝子p53の腎病態進行抑制機構の解明2011

    • Author(s)
      福田 亮介 、小山 皓介、古賀 友紹、甲斐 友佳理、メリーアン スイコ、首藤 剛、甲斐 広文
    • Organizer
      第28回日本薬学会九州支部大会
    • Place of Presentation
      福岡大学薬学部(福岡)
    • Related Report
      2011 Research-status Report
  • [Presentation] 翻訳後N型糖鎖修飾によるトランスサイレチンの新規小胞体品質管理機構2011

    • Author(s)
      庄 美里、佐藤 卓史、迫 康弘、桃原 真美子、西頭 英起、金子 雅幸、メリーアン スイコ、首藤 剛、甲斐 広文
    • Organizer
      第28回日本薬学会九州支部大会
    • Place of Presentation
      福岡大学薬学部(福岡)
    • Related Report
      2011 Research-status Report
  • [Presentation] 糖尿病性腎症に対する新たなphysical medicineの病態改善効果2011

    • Author(s)
      岡本 有加、古賀(森野) 沙緒里 、古賀 友紹 、大町 紘平、メリーアン スイコ、首藤 剛、甲斐 広文
    • Organizer
      第28回日本薬学会九州支部大会
    • Place of Presentation
      福岡大学薬学部(福岡)
    • Related Report
      2011 Research-status Report
  • [Presentation] 遺伝性腎疾患alport症候群に対する新たなphysical medicineの腎病態改善効果2011

    • Author(s)
      甲斐 友佳理、古賀 友紹 、福田 亮介、古賀(森野) 沙緒里 、メリーアン スイコ、小山 皓介、佐藤 卓史、首藤 剛、甲斐 広文
    • Organizer
      第28回日本薬学会九州支部大会
    • Place of Presentation
      福岡大学薬学部(福岡)
    • Related Report
      2011 Research-status Report
  • [Presentation] Mild electrical stimulation suppresses fat accumulation and increases stress resistance via mitochondrial membrane potentioal-dependent activation of AMP-activated protein kinase.2011

    • Author(s)
      Shingo Matsuyama, Shuichiro Yano, Saori Koga-Morino, Yuichiro Shimauchi, Yuka Okamoto, Ihori Shitanda, Mary Ann Suico, Tsuyoshi Shuto, Hirofumi Kai
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川)
    • Related Report
      2011 Research-status Report
  • [Presentation] The tumor suppressor Retinoblastoma protein positively regulates innate immune receptor Toll-like receptor 3 in epithelial cells.2011

    • Author(s)
      Kosue Koyama, Manabu Taura, Mary Ann Suico, Tsuyoshi Shuto, Seiji Okada, Hirofumi Kai
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川)
    • Related Report
      2011 Research-status Report
  • [Presentation] HRD1-dependent endoplasmic reticulum-associated degradation pathway is involved in the clearance of transthyretin variant.2011

    • Author(s)
      Misato Sho, Takashi Sato, Yasuhiro Sako, Mamiko Momohara, Hideki Nishitoh, Masayuki Kaneko, Mary Ann Suico, Tsuyoshi Shuto and Hirofumi Kai
    • Organizer
      5th International Congress on Stress Response in Biology and Medicine
    • Place of Presentation
      Loews Hotel Le Concorde,Quebec(Canada)
    • Related Report
      2011 Research-status Report
  • [Presentation] Protective effects of p53 in a mouse model of progressive hereditary kidney disease Alport syndrome.2011

    • Author(s)
      R. Fukuda, K. Koyama, T. Koga, Y. Kai, M. A. Suico, T. Shuto, H. Kai
    • Organizer
      ASCB 51st Annual Meeting
    • Place of Presentation
      Colorado Convention Center,Denver(USA)
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.molmed730.org/

    • Related Report
      2013 Final Research Report
  • [Remarks] 熊本大学薬学部遺伝子機能応用学研究室

    • URL

      http://molmed730.org/index.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] 熊本大学大学院薬学教育部 遺伝子機能応用学研究室

    • URL

      http://molmed730.org/index.html

    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://molmed730.org/

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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