| Project/Area Number |
23590084
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Gifu Pharmaceutical University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | アレルギー / 気管支喘息 / toll-like receptors / 抗原 / toll様受容体 / インターフェロン / ダニ抗原 / 好酸球 / 喘息 / 自然免疫 |
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| Research Abstract |
In the present study, we investigated whether innate immune responses such as toll-like receptors (TLRs) against a crude extract of Dermatophagoides farinae (ceDer f)are involved in the onset of atopic asthma using a mouse model of allergic asthma.
As a result, asthma-like phenotype, including airway hyper-responsiveness and airway eosinophilia, are dependent on TLR4, TLR9, ASC and type I IFN according to the data using each KO mice. In addition, the treatment of chloroquine, an inhibitor of endosome-lysosome acidification, with ceDer f clearly inhibited asthmatic phenotype in mice. Furthermore, the contamination of genomic DNA was detected in ceDer f using qPCR. In contrast, TLR9 was not involved in the asthma like phenotype using a conventional asthma model administered with ceDer f plus alum intraperitoneally followed by intratracheal injection of the allergen three times.
These findings indicated that TLRs play a pivotal role in the onset of mite-induced asthmatic phenotype.
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