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Basic research regarding the role of innate immune responses in the onset of allergic diseases

Research Project

Project/Area Number 23590084
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biological pharmacy
Research InstitutionGifu Pharmaceutical University

Principal Investigator

TANAKA Hiroyuki  岐阜薬科大学, 薬学部, 准教授 (70264695)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsアレルギー / 気管支喘息 / toll-like receptors / 抗原 / toll様受容体 / インターフェロン / ダニ抗原 / 好酸球 / 喘息 / 自然免疫
Research Abstract

In the present study, we investigated whether innate immune responses such as toll-like receptors (TLRs) against a crude extract of Dermatophagoides farinae (ceDer f)are involved in the onset of atopic asthma using a mouse model of allergic asthma.
As a result, asthma-like phenotype, including airway hyper-responsiveness and airway eosinophilia, are dependent on TLR4, TLR9, ASC and type I IFN according to the data using each KO mice. In addition, the treatment of chloroquine, an inhibitor of endosome-lysosome acidification, with ceDer f clearly inhibited asthmatic phenotype in mice. Furthermore, the contamination of genomic DNA was detected in ceDer f using qPCR. In contrast, TLR9 was not involved in the asthma like phenotype using a conventional asthma model administered with ceDer f plus alum intraperitoneally followed by intratracheal injection of the allergen three times.
These findings indicated that TLRs play a pivotal role in the onset of mite-induced asthmatic phenotype.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (10 results)

All 2012 2011 Other

All Journal Article (1 results) Presentation (7 results) Book (1 results) Remarks (1 results)

  • [Journal Article] 気道アレルギーにおける抗原感作と発症への感染の関与2012

    • Author(s)
      田中宏幸、山下弘高、稲垣直樹
    • Journal Title

      アレルギー・免疫総説

      Volume: 19 Pages: 20-25

    • Related Report
      2013 Final Research Report
  • [Presentation] ダニ抗原誘発マウス気道炎症モデルにおけるapoptosis-associated speck-like protein containing a CARD (ASC)の意義2012

    • Author(s)
      佐々木将也、加藤光、山下弘高、稲垣直樹、田中宏幸
    • Organizer
      第122回日本薬理学会近畿部会一般演題
    • Place of Presentation
      大阪
    • Year and Date
      2012-11-16
    • Related Report
      2013 Final Research Report
  • [Presentation] New frontier in the molecular mechanisms of allergic diseases. House dust mite-induced airway eosinophilic inflammation–Involvement of toll-like receptor 9-2012

    • Author(s)
      Hiroyuki Tanaka, Naoki Inagaki
    • Organizer
      第85回日本薬理学会年会シンポジウム
    • Place of Presentation
      京都
    • Year and Date
      2012-03-16
    • Related Report
      2013 Final Research Report
  • [Presentation] ダニ抗原誘発マウス気道炎症モデルにおけるapoptosis-associated speck-like protein containing a CARD (ASC)の意義2012

    • Author(s)
      佐々木将也、加藤 光、山下弘高、稲垣直樹、田中宏幸
    • Organizer
      第122回日本薬理学会近畿部会 一般演題C-15
    • Place of Presentation
      千里ライフサイエンスセンター
    • Related Report
      2012 Research-status Report
  • [Presentation] ダニ抗原誘発マウス喘息様病態形成におけるTLR9の意義2011

    • Author(s)
      升田雄士、田中宏幸、江崎友哉、村田健司、永井博弌、稲垣直樹
    • Organizer
      第61回日本アレルギー学会秋季学術大会一般演題
    • Place of Presentation
      東京
    • Year and Date
      2011-11-10
    • Related Report
      2013 Final Research Report
  • [Presentation] ダニ抗原誘発喘息様病態形成から見えてきた創薬ターゲット2011

    • Author(s)
      田中宏幸
    • Organizer
      生体機能と創薬シンポジウム2011シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2011-09-01
    • Related Report
      2013 Final Research Report
  • [Presentation] ダニ抗原誘発マウス喘息様病態形成におけるTLR9の意義

    • Author(s)
      升田雄士、田中宏幸、江崎友哉、村田健司、永井博弌、稲垣直樹
    • Organizer
      第61回日本アレルギー学会秋季学術大会
    • Place of Presentation
      グランドプリンスホテル新高輪
    • Related Report
      2011 Research-status Report
  • [Presentation] New frontiers in the molecular mechanisms of allergic diseases

    • Author(s)
      Hiroyuki Tanaka, Naoki Inagaki
    • Organizer
      第85回日本薬理学会年会(招待講演)
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2011 Research-status Report
  • [Book] アレルギー科・免疫2012

    • Author(s)
      田中宏幸、山下弘高、稲垣直樹
    • Total Pages
      172
    • Publisher
      気道アレルギーにおける抗原感作と発症への感染の関与
    • Related Report
      2011 Research-status Report
  • [Remarks] 研究室紹介ホームページ

    • URL

      http://www.gifu-pu.ac.jp/research/research_yakuri.html

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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