Molecular mechanism for malignant cell transformationinduced by a peptide derived from tenascin C through integrin activation

• Project/Area Number: 23590090
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Tokyo University of Science
• Principal Investigator: FUKAI Fumio 東京理科大学, 薬学部・生命創薬科学科, 教授 (90124487)
• Co-Investigator(Kenkyū-buntansha): IYODA Takuya 東京理科大学, 薬学部・生命創薬科学科, 助教 (80465715)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2013: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: 細胞生物学 / 細胞接着 / インテグリン / テネイシンC / 神経膠芽腫 / PDGF / 悪性腫瘍 / 細胞増殖 / 悪性転換 / テネイシン / がん

Research Abstract

Following results were obtained by the present study: 1) Peptide TNIIIA2 derived from tenascin-C (TNC) has the ability to induce potentiated and sustained activation in beta1-integrins, 2) TNIIIA2 is capable of inducing hyperstimulation of cell survival and proliferation though activation of integrins, 3) TNIIIA2 may serve as a tumor promotor. These results provide important basis for understanding of the role of TNC in tumor initiation and promotion

Research Products

• [Journal Article] The cell adhesion and proliferation activities of a peptide derived from human tenascin-C are dependent on two Ile residues (2012)
  • Author(s): Hayashi R., Miura, S., Saito Y., Osada S., Iyoda, T., Fukai, F., and Kodama H
  • Journal Title: Bioorg. Med Chem Volume: 20 Pages: 4608-4613
  • Peer Reviewed
• [Journal Article] (2012)
  • Author(s): Matsunaga T, Fukai F, Kameda T, Shide K, Shimoda H, Torii E, Kamiunten A, Sekine M, Yamamoto S, Hidaka T, Kubuki Y, Yokokura S, Uemura M, Matsuoka A, Waki F, Matsumoto K, Kanaji N, Ishii T, Imataki O, Dobashi H, Bandoh S, Shimoda K.
  • Journal Title: Ann Hamatol Volume: 91(10) Pages: 1633-1643
  • Peer Reviewed
• [Journal Article] Eukaryotic translation elongation factor 1A induces anoikis by triggering cell detachment (2012)
  • Author(s): Itagaki, K., Naito, T., Iwakiri, R., Haga, M., Miura S., Saito Y., Owaki, T., Kamiya, S., Iyoda, T., Yajima, H., Iwashita, S., Ejiri, S., and Fukai, F.
  • Journal Title: J Biol Chem Volume: 287 Pages: 16037-16046
  • Peer Reviewed
• [Journal Article] Elevated HIF-1αexpression of acute myelogenous leukemia stem cells in the endosteal hypoxic zone may be a cause of minimal residual disease in bone marrow after chemotherapy (2012)
  • Author(s): Matsunaga T, Imataki O, Torii E, Kameda T, Shide K, Shimoda H, Kamiunten A, Sekine M, Taniguchi Y, Yamamoto S, Hidaka T, Katayose K, Kubuki Y, Dobashi H, Bandoh S, Ohnishi H, Fukai F, Shimoda K.
  • Journal Title: Leukemia Res Volume: 36 Pages: 122-124
  • Peer Reviewed
• [Journal Article] Modulation of tumor cell survival, proliferation, and differentiation by the peptide derived from tenascin-C (2012)
  • Author(s): Iyoda, T., and Fukai, F
  • Journal Title: Int. J Cell Biol. 2012 Volume: Article ID 647594 Pages: 10-10
  • Peer Reviewed
• [Journal Article] Potentiated activation of VLA-4 and VLA-5 accelerates proplatelet-like formation (2012)
  • Author(s): Takuya Matsunaga
  • Journal Title: Ann Hematol Volume: 91 Pages: 1633-1643
  • Peer Reviewed
• [Journal Article] Elevated HIF-1α expression of acute myelogenous leukemia stem cells in the endosteal hypoxic zone may be a cause of minimal residual disease in bone marrow after chemotherapy (2012)
  • Author(s): Takuya Matunaga
  • Journal Title: Leukemia Res Volume: 36 Pages: 122-124
  • Peer Reviewed
• [Journal Article] Eukaryotic translation elongation factor 1A induces anoikis by triggering cell detachment. (2012)
  • Author(s): Keisuke Itagaki
  • Journal Title: J Biol Chem Volume: 287 Pages: 16037-16048
  • Peer Reviewed
• [Journal Article] Modulation of tumor cell survival, proliferation, and differentiation by the peptide derived from tenascin-C (2012)
  • Author(s): Takuya Iyoda
  • Journal Title: Int J Cell Biol Volume: 2012 Pages: 647594-647604
  • Peer Reviewed
• [Journal Article] Eukaryotic translation elongation factor 1A induces anoikis by triggering cell detachment. (2012)
  • Author(s): Itagaki, K.,
  • Journal Title: J. Biol. Chem. Volume: 287 Pages: 16037-16046
  • Peer Reviewed
• [Journal Article] Modulation of tumor cell survival, proliferation, and differentiation by the peptide derived from tenascin-C. (2012)
  • Author(s): Iyoda, T., and Fukai, F.
  • Journal Title: Int. J. Cell Biol. Volume: 2012
• [Presentation] テネイシンC分子由来のインテグリン活性化ペプチドによる神経膠芽腫細胞のPDGF依存性増殖、移動能の過剰増強 (2012)
  • Author(s): 藤田元道、藤澤達也、伊豫田拓也、深井文雄
  • Organizer: 第2回テネイシンフォーラム
  • Place of Presentation: 東京理科大学・森戸記念館
  • Year and Date: 2012-11-10
• [Presentation] 細胞接着分子を標的とした新しい治療薬の開発 (2012)
  • Author(s): 深井文雄
  • Organizer: 第23回新薬創製談話会
  • Place of Presentation: 熱海
  • Year and Date: 2012-09-11
• [Presentation] テネイシンC分子由来のインテグリン活性化ペプチドによる神経膠芽腫細胞のPDGF依存性増殖、移動能の過剰増強 (2012)
  • Author(s): 藤田元道、藤澤達也、伊豫田拓也、深井文雄
  • Organizer: 日本薬学会第132年会(北海道大学
  • Place of Presentation: 札幌(横浜)
  • Year and Date: 2012-03-29
• [Presentation] テネイシン C 由来のペプチドTNIIIA2 は PDGF 依存性の細胞増殖を過度に促進する (2011)
  • Author(s): 大塚一樹、藤澤達也、伊豫田拓也、深井文雄
  • Organizer: 第1回テネイシンフォーラム
  • Place of Presentation: 三重大学医学部
  • Year and Date: 2011-11-05
• [Presentation] Cell regulation by cryptic functional sites in fibronectin and tenascin-C molecules (2011)
  • Author(s): Fumio Fukai
  • Organizer: 10th Osteoponchin meeting
  • Place of Presentation: 北海道大学医学部、札幌
  • Year and Date: 2011-06-19
• [Presentation] Cell Regulation by Cryptic Functional Sites in Fibronectin and Tenascin-C Molecules (2011)
  • Author(s): Fumio Fukai
  • Organizer: 10th Osteopontin Meeting(招待講演)
  • Place of Presentation: Hokkaido University
• [Book] Cell regulation through membrane raft/caveolae in Electrical Phenomena at Interfaces and Biointerfaces: Fundamentals and Applications in Nano-, Bio-, and Environmental Sciences (2012)
  • Author(s): Yohei Saito, Toshiyuki Owaki, and Fumio Fukai
  • Publisher: John Wiley & Son