| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
Lipid A deacylated by outer membrane enzyme LpxR is less active than unmodified lipid A, but more active than lipid A precursor purified from LpxM mutant. These results suggest that lipid A modification by LpxR is beneficial for bacterial adaptation to host.
Outer membrane vesicle was prepared from E. coli W3110, and the structure of lipid A and its protein composition were examined. Specific lipid A modification was not observed. On the other hand, it was revealed that the outer membrane vescicle contained flagellin, a component of flagella. The ability to form flagella, including the synthesis of flagella proteins, affected the production of outer membrane vesicles.
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