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Essential role of poly(ADP-ribosyl)ation in maintenance of spermatogonial stem cells

Research Project

Project/Area Number 23590097
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biological pharmacy
Research InstitutionOsaka Ohtani University

Principal Investigator

TAKEHASHI MASANORI  大阪大谷大学, 薬学部, 准教授 (10378862)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords精子幹細胞 / ポリ(ADP-リボース) / 多能性幹細胞 / 発生・分化 / 再生医療 / ポリ(ADP-リボース) / ポリ(ADP-リボース)
Research Abstract

Spermatogonial stem cells (SSCs) can proliferate in vitro in the presence of glial cell line-derived neurotrophic factor, a self-renewal factor for SSCs. These cultured SSCs are called germline stem (GS) cells. In previous study, we examined the effect of PARP inhibitor, inhibition of poly(ADP-ribosyl)ation, on GS cells. Inhibition of poly(ADP-ribosyl)ation suppressed cell proliferation of GS cells, and induced cell death. In order to identify the causative genes that is involved in the pathway leading to the suppression of cell proliferation, the gene expression and protein phosphorylation analyses were carried out. Some genes in p53 signaling pathway were up-regulated after addition of PARP inhibitors. We then investigated activation of p53 by detection of its phosphorylation site. The phosphorylation was increased after addition of PARP inhibitors. These results suggest that poly(ADP-ribosyl)ation regulates activity of p53 and thus p53 signaling pathway in GS cells.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (15 results)

All 2014 2013 2012 2011 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (9 results)

  • [Journal Article] Hybridization of testis-derived stem cells with somatic cells and embryonic stem cells in mice2012

    • Author(s)
      Takehashi M, Tada M, Kanatsu-Shinohara M, Morimoto H, Kazuki Y, Oshimura M, Tada T, Shinohara T
    • Journal Title

      Biol Reprod

      Volume: 86

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hybridization of testis-derived stem cells with somatic cells and embryonic stem cells in Mice2012

    • Author(s)
      Takehashi, M., Tada, M., Kanatsu-Shinohara, M., Morimoto, H., Kazuki, Y., Oshimura, M., Tada, T. and Shinohara, T
    • Journal Title

      Biol. Reprod

      Volume: 86(6) Issue: 6 Pages: 178-178

    • DOI

      10.1095/biolreprod.112.098988

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mammalian selenocysteine lyase is involved in selenoprotein biosynthesis2011

    • Author(s)
      Kurokawa S, Takehashi M, Tanaka H, Mihara H, Kurihara T, Tanaka S, Hill K, Burk R, Esaki N
    • Journal Title

      J Nutr Sci Vitaminol (Tokyo)

      Volume: 57 Pages: 298-305

    • NAID

      130001373002

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of poly(ADP-ribose) polymerase-1 attenuates the toxicity of carbon tetrachloride2011

    • Author(s)
      Banasik M, Stedeford T, Strosznajder RP, Takehashi M, Tanaka S, Ueda K
    • Journal Title

      J Enzyme Inhib Med Chem

      Volume: 26 Pages: 883-889

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of poly(ADP-ribose) polymerase-1 attenuates the toxicity of carbon tetrachloride.2011

    • Author(s)
      Banasik M, et al.
    • Journal Title

      J Enzyme Inhib Med Chem

      Volume: 26 Pages: 883-889

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mammalian selenocysteine lyase is involved in selenoprotein biosynthesis.2011

    • Author(s)
      Kurokawa S, et al.
    • Journal Title

      J Nutr Sci Vitaminol (Tokyo)

      Volume: 57 Pages: 298-305

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] PARP-1による神経幹細胞の増殖制御2014

    • Author(s)
      奥田明子, 他
    • Organizer
      日本薬学会第134年会
    • Place of Presentation
      熊本
    • Year and Date
      2014-03-28
    • Related Report
      2013 Final Research Report
  • [Presentation] 多能性幹細胞の初期化能の解析2014

    • Author(s)
      中嶋瀬里奈, 他
    • Organizer
      日本薬学会第134年会
    • Place of Presentation
      熊本
    • Year and Date
      2014-03-28
    • Related Report
      2013 Final Research Report
  • [Presentation] ポリ(ADP-リボシル)化の制御が精子幹細胞に与える影響2014

    • Author(s)
      小野友久, 他
    • Organizer
      日本薬学会第134年会
    • Place of Presentation
      熊本
    • Year and Date
      2014-03-28
    • Related Report
      2013 Final Research Report
  • [Presentation] PARP-1による神経幹細胞の増殖制御2014

    • Author(s)
      奥田明子,竹橋正則,田中静吾
    • Organizer
      薬学会134年会
    • Place of Presentation
      熊本
    • Related Report
      2013 Annual Research Report
  • [Presentation] 多能性精子幹細胞の初期化能の解析2014

    • Author(s)
      中嶋瀬里奈,片山健太郎,奥田明子,田中静吾,竹橋正則
    • Organizer
      薬学会134年会
    • Place of Presentation
      熊本
    • Related Report
      2013 Annual Research Report
  • [Presentation] ポリ(ADP-リボシル)化の抑制が精子幹細胞に与える影響2014

    • Author(s)
      小野友久,中嶋瀬里奈,奥田明子,田中静吾,竹橋正則
    • Organizer
      薬学会134年会
    • Place of Presentation
      熊本
    • Related Report
      2013 Annual Research Report
  • [Presentation] PARP-1阻害剤のよるp21を介した細胞周期制御2013

    • Author(s)
      奥田明子, 他
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      横浜
    • Year and Date
      2013-03-28
    • Related Report
      2013 Final Research Report
  • [Presentation] PARP-1阻害剤が神経幹細胞の細胞周期に与える影響について2012

    • Author(s)
      前田藍佳, 他
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      札幌
    • Year and Date
      2012-03-29
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Presentation] PARP-1阻害剤によるp21を介した細胞周期制御

    • Author(s)
      奥田明子
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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