| Project/Area Number |
23590101
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Himeji Dokkyo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SHIBATA Katsushi 姫路獨協大学, 薬学部, 教授 (70296565)
SAKAI Nobuya 姫路獨協大学, 薬学部, 講師 (30525077)
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| Co-Investigator(Renkei-kenkyūsha) |
KOSHIMIZU Taka-aki 自治医科大学, 医学部, 准教授 (20392491)
IMANISHI Yorihisa 慶應義塾大学, 医学部, 講師 (80255538)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | RASSF1A / PRMT5 / 微小管 / 蛋白質アルギニンメチル化 / アルギニンメチル化酵素 |
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| Research Abstract |
We have identified protein arginine methyltransferase 5 (PRMT5) as a novel interacting protein with RASSF1A by peptide mass fingerprinting. Also, we found that co-expression of RASSF1A and PRMT5 in COS-7 cells led to partial re-localization of PRMT5 from cytosol to circular microtubule ring where it became strongly co-localized with RASSF1A. Also we found that the RASSF1A associated serine/threonine kinases; Mst1/2 and LATS, which were involved in the Hippo tumor suppressor pathway, translocated from cytosol to microtubule network on co-expression with RASSF1A. The results obtained suggest that RASSF1A links PRMT5 to microtubules and PRMT5-RASSF1A interaction plays a role in microtubule organization and that RASSF1A may play a novel role in bridging PRMT5 to arginine-methylation on microtubule, where it may play a role in modulating the RASSF1A-mediated tumor suppressive functions.
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