Development of novel antibody therapy targeting tetraspanin CD81

• Project/Area Number: 23590102
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Shujitsu University
• Principal Investigator: NAKANISHI Tohru 就実大学, 薬学部, 教授 (30243463)
• Co-Investigator(Kenkyū-buntansha): WATANABE Masahiko 就実大学, 大学院医療薬学研究科, 教授 (00182949) ; ARAI Yuji 京都府立医科大学, 医学部, 講師 (50347449) ; NAGATSUKA Hitoshi 岡山大学, 大学院医歯薬学総合研究科, 教授 (70237535)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: テトラスパニン / CD81 / siRNA / モノクローナル抗体 / サンドイッチELISA / 炎症性サイトカイン / シノビオリン / TNFalpha / アポトーシス / グリア細胞 / iPS細胞 / 関節リウマチ / ELISA

Research Abstract

Extracellular domain of CD81 was expressed in E.coli and immunized into mice. Monoclonal antibodies against CD81 were established from these mice, and antibody treatment of RA was performed by using CIA rat model. Furthermore, quantitative sandwich ELISA system was established by using recombinant CD81 and anti-CD81 antibodies. CD81siRNA introduced into SW982 cells reduced the expression of inflammatory cytikines and synoviolin.

Research Products

• [Journal Article] Necrotic and apoptotic cells serve as nuclei for calcification on osteoblastic differentiation of human mesenchymal stem cells in vitro (2014)
  • Author(s): Fujita H, Yamamoto M, Ogino T, Kubochi H, Ohmoto N, Aoyama E, Oka T, Nakanishi T, Inoue K, Sasaki J
  • Journal Title: Cell Biochem Funct Volume: 32 Pages: 77-8
• [Journal Article] Establishment of a monoclonal antibody against CD81 that decreases the proliferation of rat glioma cells (2014)
  • Author(s): Fujimoto E, Mori H, Takehara M, Tanaka M, Ohashi T, Ninomiya Y, Nakanishi T
  • Journal Title: J Hard Tissue Biol Volume: 23 Pages: 131-134
  • NAID: 130004480632
• [Journal Article] Establishment of a monoclonal antibody against CD81 that decreases the proliferation of rat glioma cells. (2014)
  • Author(s): Fujimoto E, Mori H, Takehara M, Tanaka M, Ohashi T, Ninomiya Y, Nakanishi T.
  • Journal Title: J Hard Tissue Biol Volume: 23 Pages: 131-134
  • NAID: 130004480632
  • Peer Reviewed
• [Journal Article] Necrotic and apoptotic cells serve as nuclei for calcification on osteoblastic differentiation of human mesenchymal stem cells in vitro. (2014)
  • Author(s): Fujita H, Yamamoto M, Ogino T, Kobuchi H, Ohmoto N, Aoyama E, Oka T, Nakanishi T, Inoue K, Sasaki J.
  • Journal Title: Cell Biochem Funct Volume: 32 Pages: 77-86
  • Peer Reviewed
• [Journal Article] Small interfering RNA targeting CD81 decreased the expression of tumor necrosis factor-alpha and synoviolin in SW982 cells (2013)
  • Author(s): Hanada T, Fujimoto E, Mori H, Ohashi T, Ninomiya Y, Nakanishi T
  • Journal Title: J Hard Tissue Biol Volume: 22 Pages: 195-200
  • NAID: 130004480579
• [Journal Article] Small Interfering RNA Targeting CD81 Decreased the Expression of Tumor Necrosis Factor-alpha and Synoviolin in SW982 Cells (2013)
  • Author(s): Hanada T, Fujimoto E, Mori H, Ohashi T, Ninomiya Y, Nakanishi T
  • Journal Title: J Hard Tissue Biol Volume: 22 Pages: 195-200
  • NAID: 130004480579
  • Peer Reviewed
• [Journal Article] Application of tetraspanin CD81 RNAi for diagnosis and therapy of rheumatoid arthrits (RA) (2012)
  • Author(s): Nakanishi T, Arai Y, Mori H, Nakajima T, Kubo T
  • Journal Title: Arthr Res Therap Volume: 14 Pages: 52-52
• [Journal Article] Application of tetraspanin CD81 RNAi for diagnosis and therapy of rheumatoid arthritis (RA) (2012)
  • Author(s): Nakanishi T, Arai Y, Mori H, Nakajima T, Kubo T
  • Journal Title: Arthr Res Therap Volume: 14 Pages: 52-52
  • Peer Reviewed
• [Journal Article] 就実大学・就実短期大学における食品衛生分野での教育研究 (2012)
  • Author(s): 中西 徹
  • Journal Title: 食品衛生学雑誌 Volume: 53 Pages: 298-300
  • Peer Reviewed
• [Journal Article] Effect of risedronate on osteoblast differentiation, expression of receptor activator of NF-κB ligand and apoptosis in mesenchymalstem cells (2011)
  • Author(s): Fujita H, Kurokawa K, Ogino T, Ono M, Yamamoto M, Oka T, Nakanishi T, kobayashi N, Tanaka N, Ogawa T, SUzaki E, Utsumi K, Sasaki J
  • Journal Title: Basic Clin Pharmacol Toxicol Volume: 109 Pages: 78-84
• [Journal Article] Application of tetraspanin CD81 RNAi for diagnosis and therapy of rheumatoid arthritis (RA) (2011)
  • Author(s): Nakanishi T
  • Journal Title: Arthritis Research and Therapy Volume: 14 Pages: 52-52
  • Peer Reviewed
• [Presentation] テトラスパニンCD81を標的とする新規リウマチ治療薬の開発 (2014)
  • Author(s): 中川周士、新井祐志、森宏樹、松下有美、久保俊一、中西徹
  • Organizer: 日本薬学会
  • Place of Presentation: 熊本
  • Year and Date: 2014-03-27
• [Presentation] テトラスパニンCD81を標的とした新規関節リウマチ治療薬および診断薬の開発 (2012)
  • Author(s): 中西 徹
  • Organizer: バイオテクノロジー国際会議・国際バイオEXPO
  • Place of Presentation: 東京ビッグサイト（東京）
• [Presentation] テトラスパニンCD81を標的とした新規関節リウマチ治療薬および診断薬の開発 (2011)
  • Author(s): 中西徹
  • Organizer: バイオテクノロジー国際会議・国際バイオEXPO
  • Place of Presentation: 東京
• [Presentation] Application of Tetraspanin CD81 RNAi for Diagnosis and Therapy of Rheumatoid Arthritis (RA) (2011)
  • Author(s): Nakanishi T
  • Organizer: 1st Bio-Rheumatology International Congress/8th GARN Meeting Tokyo(招待講演)
  • Place of Presentation: Tokyo
• [Presentation] テトラスパニンCD81を標的とする新規リウマチ治療薬の開発
  • Author(s): 中川周士、新井祐志、森 宏樹、松下有美、久保俊一、中西 徹
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本大学
• [Remarks] CD81診断薬に関する出願準備中