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Development of novel antibody therapy targeting tetraspanin CD81

Research Project

Project/Area Number 23590102
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biological pharmacy
Research InstitutionShujitsu University

Principal Investigator

NAKANISHI Tohru  就実大学, 薬学部, 教授 (30243463)

Co-Investigator(Kenkyū-buntansha) WATANABE Masahiko  就実大学, 大学院医療薬学研究科, 教授 (00182949)
ARAI Yuji  京都府立医科大学, 医学部, 講師 (50347449)
NAGATSUKA Hitoshi  岡山大学, 大学院医歯薬学総合研究科, 教授 (70237535)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsテトラスパニン / CD81 / siRNA / モノクローナル抗体 / サンドイッチELISA / 炎症性サイトカイン / シノビオリン / TNFalpha / アポトーシス / グリア細胞 / iPS細胞 / 関節リウマチ / ELISA
Research Abstract

Extracellular domain of CD81 was expressed in E.coli and immunized into mice. Monoclonal antibodies against CD81 were established from these mice, and antibody treatment of RA was performed by using CIA rat model. Furthermore, quantitative sandwich ELISA system was established by using recombinant CD81 and anti-CD81 antibodies. CD81siRNA introduced into SW982 cells reduced the expression of inflammatory cytikines and synoviolin.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (17 results)

All 2014 2013 2012 2011 Other

All Journal Article (11 results) (of which Peer Reviewed: 6 results) Presentation (5 results) Remarks (1 results)

  • [Journal Article] Necrotic and apoptotic cells serve as nuclei for calcification on osteoblastic differentiation of human mesenchymal stem cells in vitro2014

    • Author(s)
      Fujita H, Yamamoto M, Ogino T, Kubochi H, Ohmoto N, Aoyama E, Oka T, Nakanishi T, Inoue K, Sasaki J
    • Journal Title

      Cell Biochem Funct

      Volume: 32 Pages: 77-8

    • Related Report
      2013 Final Research Report
  • [Journal Article] Establishment of a monoclonal antibody against CD81 that decreases the proliferation of rat glioma cells2014

    • Author(s)
      Fujimoto E, Mori H, Takehara M, Tanaka M, Ohashi T, Ninomiya Y, Nakanishi T
    • Journal Title

      J Hard Tissue Biol

      Volume: 23 Pages: 131-134

    • NAID

      130004480632

    • Related Report
      2013 Final Research Report
  • [Journal Article] Establishment of a monoclonal antibody against CD81 that decreases the proliferation of rat glioma cells.2014

    • Author(s)
      Fujimoto E, Mori H, Takehara M, Tanaka M, Ohashi T, Ninomiya Y, Nakanishi T.
    • Journal Title

      J Hard Tissue Biol

      Volume: 23 Pages: 131-134

    • NAID

      130004480632

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Necrotic and apoptotic cells serve as nuclei for calcification on osteoblastic differentiation of human mesenchymal stem cells in vitro.2014

    • Author(s)
      Fujita H, Yamamoto M, Ogino T, Kobuchi H, Ohmoto N, Aoyama E, Oka T, Nakanishi T, Inoue K, Sasaki J.
    • Journal Title

      Cell Biochem Funct

      Volume: 32 Pages: 77-86

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Small interfering RNA targeting CD81 decreased the expression of tumor necrosis factor-alpha and synoviolin in SW982 cells2013

    • Author(s)
      Hanada T, Fujimoto E, Mori H, Ohashi T, Ninomiya Y, Nakanishi T
    • Journal Title

      J Hard Tissue Biol

      Volume: 22 Pages: 195-200

    • NAID

      130004480579

    • Related Report
      2013 Final Research Report
  • [Journal Article] Small Interfering RNA Targeting CD81 Decreased the Expression of Tumor Necrosis Factor-alpha and Synoviolin in SW982 Cells2013

    • Author(s)
      Hanada T, Fujimoto E, Mori H, Ohashi T, Ninomiya Y, Nakanishi T
    • Journal Title

      J Hard Tissue Biol

      Volume: 22 Pages: 195-200

    • NAID

      130004480579

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Application of tetraspanin CD81 RNAi for diagnosis and therapy of rheumatoid arthrits (RA)2012

    • Author(s)
      Nakanishi T, Arai Y, Mori H, Nakajima T, Kubo T
    • Journal Title

      Arthr Res Therap

      Volume: 14 Pages: 52-52

    • Related Report
      2013 Final Research Report
  • [Journal Article] Application of tetraspanin CD81 RNAi for diagnosis and therapy of rheumatoid arthritis (RA)2012

    • Author(s)
      Nakanishi T, Arai Y, Mori H, Nakajima T, Kubo T
    • Journal Title

      Arthr Res Therap

      Volume: 14 Pages: 52-52

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 就実大学・就実短期大学における食品衛生分野での教育研究2012

    • Author(s)
      中西 徹
    • Journal Title

      食品衛生学雑誌

      Volume: 53 Pages: 298-300

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effect of risedronate on osteoblast differentiation, expression of receptor activator of NF-κB ligand and apoptosis in mesenchymalstem cells2011

    • Author(s)
      Fujita H, Kurokawa K, Ogino T, Ono M, Yamamoto M, Oka T, Nakanishi T, kobayashi N, Tanaka N, Ogawa T, SUzaki E, Utsumi K, Sasaki J
    • Journal Title

      Basic Clin Pharmacol Toxicol

      Volume: 109 Pages: 78-84

    • Related Report
      2013 Final Research Report
  • [Journal Article] Application of tetraspanin CD81 RNAi for diagnosis and therapy of rheumatoid arthritis (RA)2011

    • Author(s)
      Nakanishi T
    • Journal Title

      Arthritis Research and Therapy

      Volume: 14 Pages: 52-52

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] テトラスパニンCD81を標的とする新規リウマチ治療薬の開発2014

    • Author(s)
      中川周士、新井祐志、森宏樹、松下有美、久保俊一、中西徹
    • Organizer
      日本薬学会
    • Place of Presentation
      熊本
    • Year and Date
      2014-03-27
    • Related Report
      2013 Final Research Report
  • [Presentation] テトラスパニンCD81を標的とした新規関節リウマチ治療薬および診断薬の開発2012

    • Author(s)
      中西 徹
    • Organizer
      バイオテクノロジー国際会議・国際バイオEXPO
    • Place of Presentation
      東京ビッグサイト(東京)
    • Related Report
      2012 Research-status Report
  • [Presentation] テトラスパニンCD81を標的とした新規関節リウマチ治療薬および診断薬の開発2011

    • Author(s)
      中西徹
    • Organizer
      バイオテクノロジー国際会議・国際バイオEXPO
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Presentation] Application of Tetraspanin CD81 RNAi for Diagnosis and Therapy of Rheumatoid Arthritis (RA)2011

    • Author(s)
      Nakanishi T
    • Organizer
      1st Bio-Rheumatology International Congress/8th GARN Meeting Tokyo(招待講演)
    • Place of Presentation
      Tokyo
    • Related Report
      2011 Research-status Report
  • [Presentation] テトラスパニンCD81を標的とする新規リウマチ治療薬の開発

    • Author(s)
      中川周士、新井祐志、森 宏樹、松下有美、久保俊一、中西 徹
    • Organizer
      日本薬学会第134年会
    • Place of Presentation
      熊本大学
    • Related Report
      2013 Annual Research Report
  • [Remarks] CD81診断薬に関する出願準備中

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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