Study on host factors involved in Hepatitis C virus life cycle
| Project/Area Number |
23590104
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
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| Project Period (FY) |
2011-04-28 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | HCV / cell muitants / C型肝炎ウイルス |
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| Outline of Final Research Achievements |
Life cycle of hepatitis C virus (HCV) is still poorly understood. In this project, I was trying to isolate host cell mutants resistant to HCV infection and to identify the defective genes in the mutants. A lot of hepatic cell mutants defective in HCV infection were established, and I found several novel host factors substantially involved in HCV infection. From the results of various analyses using established cell mutants defective in HCV infection, I also confirmed that a tight junction protein occludin is essential for both cell-free HCV entry and cell-to-cell HCV transmission in hepatic cells, and would be therefore a promising anti-HCV drug target.
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Report
(6 results)
Research Products
(30 results)
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[Journal Article] Occludin-Knockout Human Hepatic Huh7.5.1-8-Derived Cells Are Completely Resistant to Hepatitis C Virus Infection2016
Author(s)
Yoshitaka Shirasago, Yoshimi Shimizu, Isei Tanida, Tetsuro Suzuki, Ryosuke Suzuki, Kazuo Sugiyama, Takaji Wakita, Kentaro Hanada, Kiyohito Yagi, Masuo Kondoh, and Masayoshi Fukasawa
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Journal Title
Biological and Pharmaceutical Bulletin
Volume: 39
Issue: 5
Pages: 839-848
DOI
NAID
ISSN
0918-6158, 1347-5215
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Monoclonal antibodies against extracellular domains of claudin-1 block hepatitis C virus infection in a mouse model2015
Author(s)
Masayoshi Fukasawa, Shotaro Nagase, Yoshitaka Shirasago, Manami Iida, Mayo Yamashita, Kohki Endo, Kiyohito Yagi, Tetsuro Suzuki, Takaji Wakita, Kentaro Hanada, Hiroki Kuniyasu, Masuo Kondoh
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Journal Title
J Virol
Volume: 89
Issue: 9
Pages: 4866-4879
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] Inhibition of hepatitis C virus infection by mouse anti-claudin 1 monoclonal antibodies2013
Author(s)
Masayoshi Fukasawa, Shotaro Nagase, Mayo Yamashita, Manami Iida, Yoshitaka Shirasago, Akihiro Watari, Tetsuro Suzuki, Takaji Wakita, Kiyohito Yagi, Masuo Kondoh
Organizer
The 20th International Symposium on Hepatitis C Virus and Related Viruses
Place of Presentation
Melbourne, Australia
Related Report
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