An elucidation of hierarchy of a biological clock and a rhythm adjustment drug by generating a novel clock function-deficient mouse
Project/Area Number |
23590107
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Biological pharmacy
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Research Institution | Kyoto University |
Principal Investigator |
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Project Period (FY) |
2011 – 2013
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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Keywords | 視交叉上核 / 概日時計 / 時計遺伝子 / 時差 / バソプレッシン / 遺伝子改変マウス / 概日リズム / 組織特異的ノックアウト / サーカディアンリズム / 生物時計 / 組織特異的Cre / リズム異常 |
Research Abstract |
The endogenous circadian clock drives oscillations in physiology and behavior with a period of about 24 hours. When travelling rapidly across multiple time zones (jet lag), we become aware of the desynchrony between our internal clock and external light-dark cycle. Although jet lag is recognized as a chronobiological problem, its specific molecular and cellular mechanisms are poorly understood. In this study, we show that circadian rhythms of locomotor activity, clock gene expression, and body temperature rapidly re-entrained to phase-shifted light-dark cycles in mice genetically deficient in V1a and V1b receptors (V1aV1bDKO). Real-time imaging of cellular rhythms in the suprachiasmatic nucleus (SCN) slices suggested that interneuronal communication mediated by V1a and V1b confers on the SCN an intrinsic resistance to external rhythm perturbation. Pharmacological blockade of V1a and V1b in the SCN of wild-type mice accelerated the speed of recovery from jet lag.
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Report
(4 results)
Research Products
(32 results)
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[Journal Article] Mice Genetically Deficient in Vasopressin V1a and V1b Receptors Are Resistant to Jet Lag2013
Author(s)
Yamaguchi Y, Suzuki T, Mizoro Y, Kori H, Okada K, Chen Y, Fustin JM, Yamazaki F, Mizuguchi N, Zhang J, Dong X, Tsujimoto G, Okuno Y, Doi M, Okamura H
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Journal Title
Science
Volume: 342
Issue: 6154
Pages: 85-90
DOI
Related Report
Peer Reviewed
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[Journal Article] RNA-methylation-dependent RNA processing controls the speed of the circadian clock2013
Author(s)
Fustin JM, Doi M, Yamaguchi Y, Hayashi H, Nishimura S, Yoshida M, Isagawa T, Suimye-Morioka M, Kakeya H, Manabe I, Okamura H
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Journal Title
Cell
Volume: 155
Issue: 4
Pages: 793-806
DOI
Related Report
Peer Reviewed
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[Journal Article] Circadian regulation of intracellular G-protein signalling mediates inter cellular synchrony and rhythmicity in the suprachiasmatic nucleus2011
Author(s)
M.Doi, A.Ishida, A.Miyake, M.Sato, R.Komatsu, F.Yamazaki, I.Kimura, S.Tsuchiya, H.Kori, K.Seo, Y.Yamaguchi, M.Matsuo, J-M.Fustin, R.Tanaka, Y.Santo, H.Yamada, Y.Takahashi, M.Araki, K.Nakao, S.Aizawa, M.Kobayashi, K.Obrietan, G.Tsujimoto, H.Okamura
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Journal Title
Nature communication
Volume: Vol.2 article#327
Issue: 1
Pages: 327-327
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Accurate Determination of S-Phase Fraction in Proliferative Cells by Dual Fluorescence and Peroxidase Immunohistochemistry with 5-Bromo-2'-Deoxyuridine (BrdU) and Ki67 Antibodies2011
Author(s)
Tanaka R, Tainaka M, Ota T, Mizuguchi N, Kato H, Urabe S, Chen Y, Fustin JM, Yamaguchi Y, Doi M, Hamada S, Okamura H
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Journal Title
J Histochem Cytochem
Volume: 59
Issue: 8
Pages: 791-798
DOI
NAID
Related Report
Peer Reviewed
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[Presentation] 時差モデルの分子解析2012
Author(s)
山口賀章、岡村均
Organizer
第35回日本神経科学大会
Place of Presentation
名古屋国際会議場、シンポジウム
Year and Date
2012-09-21
Related Report
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