Protein tyrosine phosphorylation in the neurovascular unit in cerebrovascular disease and therapeutic strategies

Research Project

Project/Area Number	23590114
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Biological pharmacy
Research Institution	Tokyo University of Pharmacy and Life Science
Principal Investigator	TAKAGI Norio 東京薬科大学, 薬学部, 教授 (50318193)
Project Period (FY)	2011 – 2013
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000) Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	脳梗塞 / 脳血管 / チロシンリン酸化 / 脳虚血 / 血液脳関門
Research Abstract	This study sought to determine the role of protein tyrosine phosphorylation in the neurovascular unit after stroke. This study demonstrated that MPO-positive cells were increased in the surrounding brain capillaries after stroke, suggesting neutrophil infiltration. This infiltration was inhibited by src inhibitor. The increases in the levels of NOX subunit p67phox and COX-2 were also attenuated by src inhibitor. In addition, this study suggests that the pathophysiological alteration of the blood brain barrier after stroke may be regulated by tyrosine phosphorylation of PDGFR-beta in the pericytes.