| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
Current study was carried out to make clear the mechanism(s) of development of airway hyperresponsiveness in asthmatics. In bronchial smooth muscles of mice with allergic asthma, an up-regulation of RhoA protein and a down-regulation of miR-140-3p were observed. In cultured human bronchial smooth muscle cells, an up-regulation of RhoA protein was observed when the function of endogenous miR-140-3p was inhibited by its antagomir. Treatment of the cells with interleukin-13 caused an up-regulation of RhoA protein and a down-regulation of miR-140-3p. These findings suggest that RhoA protein expression is negatively regulated by miR-140-3p in bronchial smooth muscle cells. The increased expression of interleukin-13 in the airways of asthmatics might, at least in part, contribute to the reduction of miR-140-3p, resulting in an up-regulation of RhoA protein and augmented smooth muscle contraction of the airways.
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