Acquisition of location-specific information of neuronal cell death and elucidation of contribution of the microdomains of the cell

• Project/Area Number: 23590120
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Waseda University (2011, 2013-2014); Teikyo Heisei University (2012)
• Principal Investigator: EDAGAWA Yoshikuni 早稲田大学, 付置研究所, 教授 (50303607)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 細胞死 / 神経薬理学 / アポトーシス / マイクロ流体システム / ミクロ薬理学 / 薬理学 / マイクロ流体デバイス

Outline of Final Research Achievements

This study was designed by using the microsystem in order to obtain the location-specific information of cell death in single cells in culture and to examine the contribution of cell micro-domains to it. The microfluidic device developed in the present study enabled to no-invasive capture and culture of isolated single cells in the micro-environment. And the system realized to monitor the process of the cell death induced by the localized stress exposed with a laminar flow of chemical fluid. The present study suggest that a microdomain of a cell projection played a critical role in inducing the apoptotic cell death, because the chemical stress of that was confirmed to be sufficient to induce expression of activated caspase-3 as an effector caspases of apoptosis in the vicinity of the cell nucleus.

Research Products

• [Journal Article] Live imaging of cell apoptosis using FRET-based bioprobes in MEMS fabricated microculture dish (2013)
  • Author(s): R Kajiyama, Y Edagawa, M Suzuki, A Nakahara, DH Yoon, T Sekiguchi, S Shoji
  • Journal Title: Transducers Volume: 2013 Pages: 2560-2563
  • Peer Reviewed
• [Journal Article] 核内受容体 (2013)
  • Author(s): 枝川義邦
  • Journal Title: 日経ドラッグインフォメーション Volume: 190 Pages: 12-13
• [Journal Article] メラトニン作動薬 (2013)
  • Author(s): 枝川義邦
  • Journal Title: 日経ドラッグインフォメーション Volume: 193 Pages: 12-13
• [Journal Article] Local cell damage-induced apoptosis analysis on microfluidic system (2013)
  • Author(s): Kajiyama R, Watanabe T, Edagawa Y, Sekiguchi T, Shoji S
  • Journal Title: J Pharmacol Sci Volume: 121 Pages: 220-220
  • Peer Reviewed
• [Journal Article] 細胞死 (2012)
  • Author(s): 枝川義邦
  • Journal Title: 日経ドラッグインフォメーション Volume: 175 Pages: 8-9
• [Journal Article] カルシウムチャネル (2012)
  • Author(s): 枝川義邦
  • Journal Title: 日経ドラッグインフォメーション Volume: 181 Pages: 8-9
• [Journal Article] NMDA受容体と記憶 （細胞保護作用を中心に） (2012)
  • Author(s): 枝川義邦
  • Journal Title: 脳21 Volume: 15 Pages: 454-460
• [Journal Article] Local stimulation-induced cell death on microfluidic analysis: implication for intracellular transmission of apoptotic impact (2011)
  • Author(s): Y Edagawa, T Watanabe, M Fujii, K Kawai, S Shoji
  • Journal Title: Neursci Res Volume: in press
  • Peer Reviewed
• [Journal Article] Microfluidic analysis of neurodegenerative impact evoked by local atimulation-triggered apoptotic infoemation (2011)
  • Author(s): Y Edagawa, T Watanabe, M Fujii, K Kawai, S Shoji
  • Journal Title: Micro Tot Anal Sys Volume: in press
  • Peer Reviewed
• [Presentation] 記憶形成におけるNMDA受容体の役割と分子病態 (2015)
  • Author(s): 枝川義邦
  • Organizer: AD Round Table Discussion in Yokohama
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2015-03-05
  • Invited
• [Presentation] アルツハイマー型認知症治療におけるNMDA受容体をターゲットにした分子アプローチ (2014)
  • Author(s): 枝川義邦
  • Organizer: 認知症カンファレンス
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2014-12-17
  • Invited
• [Presentation] Live imaging of cell apoptosis using FRET-based bioprobes in MEMS fabricated microculture dish (2013)
  • Author(s): R Kajiyama, Y Edagawa, M Suzuki, A Nakahara, DH Yoon, T Sekiguchi, S Shoji
  • Organizer: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems
  • Place of Presentation: Barcelona, Spain
• [Presentation] マイクロ流体システムを用いた局所的損傷刺激によるアポトーシス解析 (2013)
  • Author(s): 梶山理沙、渡辺卓、枝川義邦、関口哲志、庄子習一
  • Organizer: 第86回日本薬理学会
  • Place of Presentation: 福岡国際会議場（福岡県）
• [Presentation] 細胞の形態と生育領域を制御する培養系の開発と細胞内イベントの可視化 (2013)
  • Author(s): 梶山理沙、枝川義邦、鈴木美穂、関口哲志、庄子習一
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: パシフィコ横浜（神奈川県）
• [Presentation] Microfluidic analysis of neurodegenerative impact evoked by local stimulation-triggered apoptotic information (2011)
  • Author(s): Y Edagawa, T Watanabe, M Fujii, K Kawai, S Shoji
  • Organizer: The 15th International Conference on Miniaturize Systems for Chemistry and Life Sciences
  • Place of Presentation: 米国・ワシントン州・シアトル
• [Presentation] From a part to the whole: Microsystems analysis of mechanism to control the fate of the cell (2011)
  • Author(s): Y Edagawa
  • Organizer: UCLA Seminar at SEMEL Institute(招待講演)
  • Place of Presentation: 米国・カリフォルニア州・ロサンジェルス
• [Presentation] アルツハイマー病の分子病態とNMDA受容体機能の調節効果
  • Author(s): 枝川義邦
  • Organizer: CHIBA Memary Alzheimer’s Disease Conference
  • Place of Presentation: 千葉県千葉市
• [Presentation] アルツハイマー病の分子病態と治療：NMDA型グルタミン酸受容体の障害性と阻害薬の効果
  • Author(s): 枝川義邦
  • Organizer: 香川県医学会
  • Place of Presentation: 香川県坂出市
• [Presentation] 記憶形成におけるNMDA受容体の役割
  • Author(s): 枝川義邦
  • Organizer: 山武郡市医師会講演会
  • Place of Presentation: 山武群市
  • Invited
• [Presentation] 認知症における記憶障害とNMDA受容体調節の効果
  • Author(s): 枝川義邦
  • Organizer: 第2回成田認知症カンファレンス
  • Place of Presentation: 成田市
  • Invited
• [Presentation] 記憶形成の分子メカニズムと認知症治療におけるNMDA受容体からのアプローチ
  • Author(s): 枝川義邦
  • Organizer: 東葛ADカンファランス
  • Place of Presentation: 柏市
  • Invited
• [Presentation] アルツハイマー型認知症における記憶障害とNMDA型グルタミン酸受容体の調節効果について
  • Author(s): 枝川義邦
  • Organizer: 千葉市認知症地域連携ADカンファランス
  • Place of Presentation: 千葉市幕張
  • Invited