Acquisition of location-specific information of neuronal cell death and elucidation of contribution of the microdomains of the cell
Project/Area Number |
23590120
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Biological pharmacy
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Research Institution | Waseda University (2011, 2013-2014) Teikyo Heisei University (2012) |
Principal Investigator |
|
Project Period (FY) |
2011-04-28 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | 細胞死 / 神経薬理学 / アポトーシス / マイクロ流体システム / ミクロ薬理学 / 薬理学 / マイクロ流体デバイス |
Outline of Final Research Achievements |
This study was designed by using the microsystem in order to obtain the location-specific information of cell death in single cells in culture and to examine the contribution of cell micro-domains to it. The microfluidic device developed in the present study enabled to no-invasive capture and culture of isolated single cells in the micro-environment. And the system realized to monitor the process of the cell death induced by the localized stress exposed with a laminar flow of chemical fluid. The present study suggest that a microdomain of a cell projection played a critical role in inducing the apoptotic cell death, because the chemical stress of that was confirmed to be sufficient to induce expression of activated caspase-3 as an effector caspases of apoptosis in the vicinity of the cell nucleus.
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Report
(5 results)
Research Products
(22 results)