| Project/Area Number |
23590121
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Doshisha Women's College of Liberal Arts |
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Principal Investigator |
FUJII Takeshi 同志社女子大学, 薬学部, 教授 (80255380)
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| Co-Investigator(Kenkyū-buntansha) |
TAKATORI Yuki 同志社女子大学, 薬学部, 助教 (90411090)
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| Co-Investigator(Renkei-kenkyūsha) |
KAWASHIMA Koichiro 北里大学, 薬学研究所, 客員教授 (70095008)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | アセチルコリン / Tリンパ球 / Ｂリンパ球 / コリンアセチルトランスフェラーゼ / 液胞型プロトンポンプ / メディエートフォア / Ｔリンパ球 / Acetylcholine / vacuolar ATPase / T cell / mediatophore / nicotinic receptor / phytohemagglutinin / リンパ球 / ニコチン受容体 / ムスカリン受容体 / ラジオイムノアッセイ |
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| Research Abstract |
We investigated interaction between vacuolar type H+ ATPase (V-ATPase) and lymphocytic cholinergic system in regulation of immune activity. In addition of mediatophore, which is a homooligomer with a 16-kDa subunit homologous to proteolipid subunit c of the V0 sector of V-ATPase, expression of several subunits (a,b,c,d,e) of the V0 sector mRNAs, which is a hydrophobic transmembrane sector of the V-ATPase, were detected in two human leukemic T cell lines CCRF-CCRF-CEM and MOLT-3, and MNLs of human and mouse. T cell activation induced a slight increase in expression of V-ATPase subunits. Anti-mediatophore siRNA decreased mediatophore mRNA expression and release of acetylcholine (ACh) from T cells, without reducing expression of choline acetyltransferase, a synthesizing enzyme of ACh. These findings indiucate that T cells express several subunits of V-ATPase V0 component, and acetylcholine release from T cells is regulated by V-ATPase.
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