Establishment of the disinfection technique with hydroxyl radicals and catalase inhibitor based on the reaction kinetic analysis

• Project/Area Number: 23590144
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Environmental pharmacy
• Research Institution: Tohoku University
• Principal Investigator: NIWANO Yoshimi 東北大学, 歯学研究科(研究院), 教授 (40375184)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 過酸化水素光分解殺菌法 / 水酸化ラジカル / カタラーゼ阻害 / 生薬 / 植物エキス / ポリフェノール / ポリフェノール光酸化 / 過酸化水素 / ヒドロキシルラジカル / カタラーゼ阻害剤

Research Abstract

Disinfection treatment with photolysis of hydrogen peroxide could be a novel alternative to existing antibiotics because the risk of inducing bacterial resistance by the disinfection treatment was proven to be quite low. In addition, we revealed that bactericidal activity of photo-irradiated hydrogen peroxide was synergistically augmented in the presence of polyphenol containing-herbal medicines and -plant extracts. The bactericidal activity of the disinfection treatment is due to hydroxyl radicals generated by photolysis of hydrogen peroxide which disappear immediately after the cessation of photo-irradiation. Therefore, there would be no problematic issues on residual toxicity, and there would be an advantage that antioxidant activity of the herbal medicines and plant extracts would alleviate the oxidative damage that might be caused by hydroxyl radicals during photo-irradiation.

Research Products

• [Journal Article] In vitro evaluation of the risk of inducing bacterial resistance to disinfection treatment with photolysis of hydrogen peroxide (2013)
  • Author(s): Ikai, H., Odashima, Y., Kanno, T., Nakamura, K., Shirato, M., Sasaki, K., Niwano, Y
  • Journal Title: PLoS ONE Volume: 8(11) Issue: 11 Pages: e81316-e81316
  • DOI: 10.1371/journal.pone.0081316
  • Peer Reviewed
• [Journal Article] Synergistic Effect of Proanthocyanidin on the Bactericidal Action of the Photolysis of H<SUB>2</SUB>O<SUB>2</SUB> (2013)
  • Author(s): Ikai, H., Nakamura, K., Kanno, T., Shirato. M., Meirelles, L., Sasaki, K., Niwano, Y
  • Journal Title: Biocontrol Science Volume: 18 Issue: 3 Pages: 137-141
  • DOI: 10.4265/bio.18.137
  • NAID: 10031202904
  • ISSN: 1342-4815, 1884-0205
  • Peer Reviewed
• [Journal Article] <i>In Vitro</i> Bactericidal Activity of Photo-Irradiated Oxydol Products via Hydroxyl Radical Generation (2013)
  • Author(s): Oyamada, A., Ikai, H., Nakamura, K., Hayashi, E., Kanno, T., Sasaki, K., Niwano, Y
  • Journal Title: Biocontrol Science Volume: 18 Issue: 2 Pages: 83-88
  • DOI: 10.4265/bio.18.83
  • NAID: 10031184509
  • ISSN: 1342-4815, 1884-0205
  • Peer Reviewed
• [Journal Article] Photo-irradiation of proanthocyanidin as a new disinfection technique via reactive oxygen species formation (2013)
  • Author(s): Nakamura K, Shirato M, Ikai H, Kanno T, Sasaki K, Kohno M, Niwano Y.
  • Journal Title: PLoS ONE Volume: 8 Issue: 3 Pages: e60053-e60053
  • DOI: 10.1371/journal.pone.0060053
  • Peer Reviewed
• [Journal Article] Synergistic Effect of Proanthocyanidin on the Bactericidal Action of the Photolysis of H2O2 (2013)
  • Author(s): Ikai H, Nakamura K, Kanno T, Shirato M, Meirelles L, Sasaki K, Niwano Y
  • Journal Title: Biocontrol Science Volume: 印刷中
  • NAID: 10031202904
  • Peer Reviewed
• [Journal Article] In vitro and in vivo anti-Staphylococcus aureus activities of a new disinfection system utilizing photolysis of hydrogen peroxide (2012)
  • Author(s): Hayashi, E., Mokudai, T., Yamada, Y., Nakamura, K., Kanno, T., Sasaki, K., Niwano, Y
  • Journal Title: J. Biosci. Bioeng Volume: 114(2) Issue: 2 Pages: 193-197
  • DOI: 10.1016/j.jbiosc.2012.03.020
  • NAID: 110009495428
  • Peer Reviewed
• [Journal Article] Topical treatment of oral cavity and wounded skin with a new disinfection system utilizing photolysis of hydrogen peroxide in rats (2012)
  • Author(s): Yamada, Y., Mokudai, T., Nakamura, K., Hayashi, E., Kawana, Y., Kanno, T., Sasaki, K., Niwano, Y
  • Journal Title: The Journal of Toxicological Sciences Volume: 37 Issue: 2 Pages: 329-335
  • DOI: 10.2131/jts.37.329
  • NAID: 10030126757
  • ISSN: 0388-1350, 1880-3989
  • Peer Reviewed
• [Journal Article] Bactericidal action of photo-irradiated gallic acid via reactive oxygen species formation (2012)
  • Author(s): Nakamura K, Yamada Y, Ikai H, Kanno T, Sasaki K, Niwano Y.
  • Journal Title: Journal of Agricultural and Food Chemistry Volume: 60 Issue: 40 Pages: 10048-10054
  • DOI: 10.1021/jf303177p
  • Peer Reviewed
• [Presentation] 過酸化水素光分解殺菌法の耐性誘導試験 (2013)
  • Author(s): 猪飼紘代、中村圭祐、小田島優、白土翠、菅野太郎、庭野吉己
  • Organizer: 日本防菌防黴学会第40回年次大会
  • Place of Presentation: 大阪
  • Year and Date: 2013-09-10
• [Presentation] Advantages of new disinfection treatment utilizing photolysis of hydrogen peroxide - Low risk of inducing bacterial resistance and postantibiotic effect– (2013)
  • Author(s): Ikai, H., Odashima, Y., Nakamura, K., Shirato, M., Kanno, T., Sasaki, K., Niwano, Y
  • Organizer: the Innovative Research for Biosis-Abiosis Intelligent Interface Summer Seminar 2013
  • Place of Presentation: Sendai
  • Year and Date: 2013-08-29
• [Presentation] 過酸化水素光分解殺菌技術を応用した新規歯周病治療器の開発 (2013)
  • Author(s): 菅野太郎、中村圭祐、猪飼紘代、庭野吉己、佐々木啓一
  • Organizer: 日本歯周病学会2013春季学術大会
  • Place of Presentation: タワーホール船堀（東京）
  • Invited
• [Presentation] Advantages of new disinfection treatment utilizing photolysis of hydrogen (2013)
  • Author(s): Ikai, H., Odashima, Y., Nakamura, K., Shirato, M., Kanno, T., Sasaki, K., Niwano, Y
  • Organizer: 8th International Workshop on Biomaterials in Interface Science
  • Place of Presentation: 遠刈田温泉さんさ亭（宮城蔵王）
• [Presentation] 過酸化水素光分解殺菌法の耐性誘導試験 (2013)
  • Author(s): 猪飼紘代 、中村圭祐、小田島優、白土翠、菅野太郎、庭野吉己
  • Organizer: 日本防菌防黴学会第40回年次大会
  • Place of Presentation: 千里ライフサイエンスセンター（豊中）
• [Presentation] 各種ポリフェノールに対する青色可視光照射で得られる殺菌効果の比較検討 (2013)
  • Author(s): 中村圭祐、石山希里香、猪飼紘代、菅野太郎、庭野吉己
  • Organizer: 日本防菌防黴学会第40回年次大会
  • Place of Presentation: 千里ライフサイエンスセンター（豊中）
• [Presentation] 市販オキシドールの光分解殺菌効果 (2012)
  • Author(s): 小山田晃、猪飼紘代、中村圭祐、菅野太郎、佐々木啓一、庭野吉己
  • Organizer: 日本防菌防黴学会第39回年次大会
  • Place of Presentation: 東京
  • Year and Date: 2012-09-12
• [Presentation] カテキン類・生薬の添加による過酸化水素光分解殺菌の併用効果 (2012)
  • Author(s): 猪飼紘代、白土翠、中村圭祐、菅野太郎、佐々木啓一、庭野吉己
  • Organizer: 日本防菌防黴学会第39回年次大会
  • Place of Presentation: 東京
  • Year and Date: 2012-09-12
• [Presentation] 過酸化水素光分解を応用したラジカル殺菌技術のin vivo殺菌活性および安全性評価 (2012)
  • Author(s): 庭野吉己、林栄成、山田康友、目代貴之、中村圭祐、菅野太郎、佐々木啓一
  • Organizer: 日本防菌防黴学会第39回年次大会
  • Place of Presentation: 東京
  • Year and Date: 2012-09-11
• [Presentation] In vivo evaluation of a new disinfection system utilizing photolysis of hydrogen peroxide (2012)
  • Author(s): Niwano, Y., Hayashi, E., Yamada, Y., Mokudai, T., Nakamura, K., Ikai, H., Kanno, T., Sasaki, K
  • Organizer: the Innovative Research for Biosis-Abiosis Intelligent Interface Summer Seminar 2012
  • Place of Presentation: Sendai
  • Year and Date: 2012-08-03
• [Presentation] 過酸化水素光分解を応用した新しい殺菌システムの黄色ブドウ球菌に対するin vitroおよびin vivo抗菌活性 (2012)
  • Author(s): 林栄成、目代貴之、山田康友、中村圭祐、菅野太郎、佐々木啓一、庭野吉己
  • Organizer: 第65回日本酸化ストレス学会学術集会
  • Place of Presentation: 徳島
  • Year and Date: 2012-06-08
• [Presentation] 過酸化水素光分解を利用した新規歯科治療器のラット口腔粘膜および創傷皮膚への局所応用における急性の毒性学的評価 (2012)
  • Author(s): 山田康友、目代貴之、中村圭祐、林栄成、菅野太郎、佐々木啓一、庭野吉己
  • Organizer: 第65回日本酸化ストレス学会学術集会
  • Place of Presentation: 徳島
  • Year and Date: 2012-06-08
• [Presentation] ポリフェノールへの青色可視光照射により生成される活性酸素を応用した新規殺菌技術 (2012)
  • Author(s): 中村圭祐、山田康友、猪飼紘代、菅野太郎、佐々木啓一、庭野吉己
  • Organizer: 日本防菌防黴学会第39回年次大会
  • Place of Presentation: 東京
• [Presentation] 過酸化水素光分解殺菌法に対する微生物の抵抗性とカタラーゼ活性の関連性 (2012)
  • Author(s): 中村圭祐、菅野太郎、目代貴之、林栄成、猪飼紘代、佐々木啓一、庭野吉己
  • Organizer: 第22回日本歯科医学会総会
  • Place of Presentation: 大阪
• [Presentation] 過酸化水素光分解殺菌法における微生物の反応動態解析 (2011)
  • Author(s): 林栄成、中村圭祐、菅野太郎、目代貴之、岩沢篤朗、河野雅弘、庭野吉己
  • Organizer: 第64回日本酸化ストレス学会
  • Place of Presentation: 北海度ルスツ
  • Year and Date: 2011-07-02
• [Presentation] 過酸化水素光分解殺菌法における微生物の反応動態解析 (2011)
  • Author(s): 林栄成、中村圭祐、菅野太郎、目代貴之、岩沢篤郎、河野雅弘、庭野吉己
  • Organizer: 第６４回日本酸化ストレス学会
  • Place of Presentation: 北海道ルスツ
• [Presentation] 過酸化水素光分解により生じるヒドロキシルラジカルの細胞毒性評価 (2011)
  • Author(s): 猪飼紘代、中村圭祐、菅野太郎、佐々木啓一、河野雅弘、庭野吉己
  • Organizer: 第６４回日本酸化ストレス学会
  • Place of Presentation: 北海道ルスツ
• [Remarks]
  • URL: http://www.redokuwa.dent.tohoku.ac.jp
• [Remarks] 庭野研究室 東北大学大学院歯学研究科 生体適合性計測工学講座
  • URL: http://www.redokuwa.dent.tohoku.ac.jp
• [Remarks] 庭野研究室 東北大学大学院歯学研究科 生体適合性計測工学講座
  • URL: http://www.redokuwa.dent.tohoku.ac.jp