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Elucidation of drug resistance mechanism against influenza virus

Research Project

Project/Area Number 23590145
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Environmental pharmacy
Research InstitutionThe University of Tokyo

Principal Investigator

Tagawa Yuko  東京大学, 医科学研究所, 助手 (40178538)

Project Period (FY) 2011-04-28 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsインフルエンザ / 薬剤耐性 / 新型インフルエンザ / 抗インフルエンザ薬 / ノイラミニダーゼ阻害薬 / 小児ぜんそく / ステロイド剤 / 迅速診断キット / 高病原性鳥インフルエンザ / インフルエンザウイルス / サーベイランス / 迅速診断 / NA阻害剤
Outline of Final Research Achievements

In clinical specimens from hundreds of patients who infected with Influenza virus (A/H1N1pdm) and received anti-influenza drug therapy, quantifying the virus and analyzing the nucleotide sequences of the drug-resistant mutant viruses were carried out. It was succeeded to show that neuraminidase inhibitor was more effective to viral shedding of H1N1pdm than that of H3N2 or B-type, but resistance mutation was observed in 10~30%. In addition, the steroid treatment for immunosuppression resulted in the viral shedding period was longer and emerging the drug-resistant virus was also remain in the long period in the case of the H1N1pdm-infected children.

Report

(6 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (23 results)

All 2016 2015 2014 2013 2012 2011 Other

All Journal Article (12 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 11 results,  Open Access: 5 results,  Acknowledgement Compliant: 4 results) Presentation (7 results) Remarks (4 results)

  • [Journal Article] インフルエンザ迅速診断キットの性能について2016

    • Author(s)
      坂井(田川)優子、河岡義裕
    • Journal Title

      インフルエンザ

      Volume: 58 Pages: 43-50

    • Related Report
      2015 Annual Research Report
  • [Journal Article] C646, a Novel p300/CREB-Binding Protein-Specific Inhibitor of Histone Acetyltransferase, Attenuates Influenza A Virus Infection.2015

    • Author(s)
      Zhao D, Fukuyama S, Sakai-Tagawa Y, Takashita E, Shoemaker JE, Kawaoka Y.
    • Journal Title

      Antimicrob Agents Chemother.

      Volume: 60 Issue: 3 Pages: 1902-6

    • DOI

      10.1128/aac.02055-15

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Comparison between virus shedding and fever duration after treating children with pandemic A H1N1/09 and children with A H3N2 with a neuraminidase inhibitor.2015

    • Author(s)
      Sugaya N, Sakai-Tagawa Y, Bamba M, Yasuhara R, Yamazaki M, Kawakami C, Yamaguchi Y, Ide Y, Ichikawa M, Mitamura K, Kawaoka Y.
    • Journal Title

      Antivir Ther.

      Volume: 20 Issue: 1 Pages: 49-55

    • DOI

      10.3851/imp2798

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Identification of PB2 mutations responsible for the efficient replication of H5N1 influenza viruses in human lung epithelial cells.2015

    • Author(s)
      Yamaji R, Yamada S, Le MQ, Li C, Chen H, Qurnianingsih E, Nidom CA, Ito M, Sakai-Tagawa Y, Kawaoka Y.
    • Journal Title

      J Virol.

      Volume: 89 Issue: 7 Pages: 3947-3956

    • DOI

      10.1128/jvi.03328-14

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Mammalian Adaptive Mutations of the PA Protein of Highly Pathogenic Avian H5N1 Influenza Virus.2015

    • Author(s)
      Yamaji R, Yamada S, Le MQ, Ito M, Sakai-Tagawa Y, Kawaoka Y.
    • Journal Title

      J Virol.

      Volume: 89 Issue: 8 Pages: 4117-4125

    • DOI

      10.1128/jvi.03532-14

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Disease severity is associated with differential gene expression at the early and late phases of infection in nonhuman primates infected with different H5N1 highly pathogenic avian influenza viruses.2014

    • Author(s)
      Muramoto Y, Shoemaker JE, Le MQ, Itoh Y, Tamura D, Sakai-Tagawa Y, Imai H, Uraki R, Takano R, Kawakami E, Ito M, Okamoto K, Ishigaki H, Mimuro H, Sasakawa C, Matsuoka Y, Noda T, Fukuyama S, Ogasawara K, Kitano H, Kawaoka Y.
    • Journal Title

      Journal of Virlogy

      Volume: 88(16) Issue: 16 Pages: 8981-8997

    • DOI

      10.1128/jvi.00907-14

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Detection sensitivity of influenza rapid diagnostic tests.2014

    • Author(s)
      Sakai-Tagawa Y, Ozawa M, Yamada S, Uchida Y, Saito T, Takahashi K, Sugaya N, Tashiro M, Kawaoka Y.
    • Journal Title

      Microbiol Immunol.

      Volume: 58 Issue: 10 Pages: 600-606

    • DOI

      10.1111/1348-0421.12185

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Influenza virus-host interactome screen as a platform for antiviral drug development.2014

    • Author(s)
      Watanabe T, Kawakami E, Shoemaker JE, Lopes TJ, Matsuoka Y, Tomita Y, Kozuka-Hata H, Gorai T, Kuwahara T, Takeda E, Nagata A, Takano R, Kiso M, Yamashita M, Sakai-Tagawa Y, Katsura H, Nonaka N, Fujii H, Fujii K, Sugita Y, Noda T, Goto H, Fukuyama S, Watanabe S, Neumann G, Oyama M, Kitano H, Kawaoka Y.
    • Journal Title

      Cell Host and Microbe

      Volume: 16 Issue: 6 Pages: 795-805

    • DOI

      10.1016/j.chom.2014.11.002

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Characterization of H7N9 influenza A viruses isolated from humans.2013

    • Author(s)
      Watanabe T, Kiso M, Fukuyama S, Nakajima N, Imai M, Yamada S, Murakami S, Yamayoshi S, Iwatsuki-Horimoto K, Sakoda Y, Takashita E, McBride R, Noda T, Hatta M, Imai H, Zhao D, Kishida N, Shirakura M, de Vries RP, Shichinohe S, Okamatsu M, Tamura T, Tomita Y , Fujimoto N, Goto K, Katsura H, Kawakami E, Ishikawa I, Watanabe S, Ito M, Sakai-Tagawa Y, Sugita Y, Uraki R, Yamaji R, Eisfeld AJ, Zhong G, Fan S, Ping J, Maher EA, Hanson A, Uchida Y, Saito T, Ozawa M, Neumann G, Kida H, Odagiri T, Paulson JC, Hasegawa H, Tashiro M, Kawaoka Y
    • Journal Title

      Nature

      Volume: 501(7468) Issue: 7468 Pages: 551-5

    • DOI

      10.1038/nature12392

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] One-Step Detection of the 2009 Pandemic Influenza A(H1N1) Virus by the RT-SmartAmp Assay and Its Clinical Validation2012

    • Author(s)
      Y. Kawai, Y. Kimura, A. Lezhava, H. Kanamori,. K. Usui, T.Hanami, T. Soma, J.Morlighem1 S. Saga, Y. Ishizu, S. Aoki, Y Sakai-Tagawa,
    • Journal Title

      PLoS One

      Volume: 7(1) Issue: 1 Pages: e30236-e30236

    • DOI

      10.1371/journal.pone.0030236

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mutation Analysis of 2009 Pandemic Influenza A(H1N1) Viruses Collected in Japan during the Peak Phase of the Pandemic2011

    • Author(s)
      Morlighem JÉ, Aoki S, Kishima M, Hanami M, Ogawa C, Jalloh A, Takahashi Y, Kawai Y, Saga S, Hayashi
    • Journal Title

      PLoS One.

      Volume: 6(4) Issue: 4 Pages: e18956-e18956

    • DOI

      10.1371/journal.pone.0018956

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Sero-prevalence of pandemic (H1N1) 2009 influenza A virus among schoolchildren and their parents in Tokyo, Japan2011

    • Author(s)
      Iwatsuki-Horimoto, K., et al.
    • Journal Title

      Clin.Vaccine Immunol.

      Volume: 18 Issue: 5 Pages: 860-866

    • DOI

      10.1128/cvi.00428-10

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 高病原性H5N1鳥インフルエンザウイルスに感染したカニクイザルの宿主応答2014

    • Author(s)
      村本裕紀子、Jason E Shoemaker、Mai Quynh Le、伊藤靖、田村大輔、坂井(田川)優子、今井博貴、岡本貴世子、石垣宏仁、小笠原一誠、北野宏明、河岡義裕
    • Organizer
      第62回日本ウイルス学会学術集会
    • Place of Presentation
      横浜市パシフィコ横浜
    • Year and Date
      2014-11-12
    • Related Report
      2014 Research-status Report
  • [Presentation] H5N1高病原性鳥インフルエンザウイルスのヒトへの適応に関与するアミノ酸変異の同定2014

    • Author(s)
      山地玲奈、山田晋弥、Mai Quyh Le、伊藤睦美、坂井(田川)優子、河岡義裕
    • Organizer
      第62回日本ウイルス学会学術集会
    • Place of Presentation
      横浜市パシフィコ横浜
    • Year and Date
      2014-11-11
    • Related Report
      2014 Research-status Report
  • [Presentation] 高病原性H5N1鳥インフルエンザウイルスに感染したカニクイザルの宿主応答2014

    • Author(s)
      村本 裕紀子、Shoemaker Jason 、Le Thi Quynh Mai 、伊藤 靖 、田村 大輔、坂井(田川) 優子、今井 博貴、岡本 貴世子、石垣 宏仁、小笠原 一誠、北野 宏明、河岡 義裕
    • Organizer
      第157回日本獣医学会学術集会
    • Place of Presentation
      札幌市北海道大学
    • Year and Date
      2014-09-11
    • Related Report
      2014 Research-status Report
  • [Presentation] 2010年から2011年に西日本で鳥から分離されたH5N1高病原性鳥インフルエンザウイルスのマウスにおける病原性の比較2013

    • Author(s)
      伊藤睦美、木曽真紀、浦木隆太、坂井(田川)優子、曽田公輔、笛吹達史、伊藤啓史、山口剛士、伊藤壽啓、河岡義裕
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2013 Research-status Report
  • [Presentation] Sero-Prevalence of Pandemic(H1N1)2009 Influenza A Virus amang schoolchildren and their parents in Tokyo,Japan.2011

    • Author(s)
      K. Iwatsuki-Horimoto,他20人
    • Organizer
      第59回日本ウイルス学会、国際微生物連合2011会議合同開催
    • Place of Presentation
      札幌、コンベンションセンター
    • Related Report
      2011 Research-status Report
  • [Presentation] 鳥インフルエンザA(H7N9)ウイルスは迅速診断キットで判定可能か?

    • Author(s)
      坂井(田川)優子、高山育代、景山勉、内田裕子、西藤岳彦、田代眞人、河岡義裕
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2013 Research-status Report
  • [Presentation] 2010/11シーズン小児臨床検体中の薬剤耐性インフルエンザウイルスの探索

    • Author(s)
      坂井(田川)優子
    • Organizer
      第60回日本ウイルス学会学術集会
    • Place of Presentation
      大阪国際会議場
    • Related Report
      2012 Research-status Report
  • [Remarks] 東京大学医科学研究所ウイルス感染分野

    • URL

      http://www.ims.u-tokyo.ac.jp/virology/index.html

    • Related Report
      2014 Research-status Report
  • [Remarks] 日経メディカル パンデミックに挑む トピックス

    • URL

      http://medical.nikkeibp.co.jp/leaf/all/special/pandemic/topics/201212/528042.html

    • Related Report
      2014 Research-status Report
  • [Remarks] 東京大学医科学研究所ウイルス感染分野ホームページ

    • URL

      http://www.ims.u-tokyo.ac.jp/virology/index.html

    • Related Report
      2012 Research-status Report
  • [Remarks] 日経メディカルオンラインに研究成果の記事掲載

    • URL

      http://medical.nikkeibp.co.jp/leaf/all/special/pandemic/topics/201212/528042.html

    • Related Report
      2012 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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