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Induction of latent biosynthetic activities of secondary metabolites in higher plants as the novel medicinal resources of natural products

Research Project

Project/Area Number 23590146
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Environmental pharmacy
Research InstitutionUniversity of Toyama

Principal Investigator

KUROSAKI FUMIYA  富山大学, 医学薬学研究部(薬学), 教授 (70143865)

Co-Investigator(Kenkyū-buntansha) KATOH Yasuo  富山県立大学, 工学部, 教授 (20254237)
OGITA Shinjiro  富山県立大学, 工学部, 准教授 (50363875)
Project Period (FY) 2011-04-28 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords植物 / バイオテクノロジー / 物質生産 / 遺伝子 / 有用資源 / 情報伝達機構 / 天然物 / 二次代謝
Outline of Final Research Achievements

The aim of the present study is the elucidation of the signal transduction mechanism of the induction of secondary metabolic activity induced by the stimulation of a plant hormone, jasmonic acid. Several cDNA cloned encoding Rac GTPase, a family of monomeric GTP-binding proteins specifically observed in higher plants, were isolated, and a homologue of which translate product should function in jasmonate-signaling was identified. Several medicinal plants were transformed by the Rac GTPase gene, and it was found that these transgenic plants showed high productivities of the useful natural products. These results suggested that the engineering of jasmonates-signaling processes in plant cells by transformation with appropriate genes would be a novel method for the transcriptional activation of several biosynthetic enzymes involved in secondary metabolism of various useful plants.

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (28 results)

All 2015 2014 2013 2012 2011 Other

All Journal Article (20 results) (of which Peer Reviewed: 18 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (8 results)

  • [Journal Article] Induction, cloning and functional expression of a sesquiterpene biosynthetic enzyme, delta-guaiene synthase, of Aquilaria microcarpa cell cultures.2014

    • Author(s)
      Lee, J. B., Hirohashi, S., Yamamura, Y., Taura, F. & Kurosaki, F.
    • Journal Title

      Natural Product Communications

      Volume: 9 Pages: 1231-1236

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Cloning and expression of putative Rac/Rop GTPase genes, Am-rac1 and Am-rac2, involved in methyl jasmonate-induced transcriptional activation of farnesyl diphosphate synthase in cell cultures of Aquilaria microcarpa.2013

    • Author(s)
      Kenmotsu, Y., Asano, K., Yamamura, Y. & Kurosaki, F.
    • Journal Title

      Plant Mol. Biol. Rep.

      Volume: 31 Issue: 3 Pages: 539-546

    • DOI

      10.1007/s11105-012-0529-0

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Molecular cloning and functional characterization of an NADPH:cytochrome P450 reductase from a tropical medicinal plant Scoparia dulcis.2013

    • Author(s)
      Yamamura, Y., Mabuchi, A. & Kurosaki F.
    • Journal Title

      Planta Medica

      Volume: 79 Pages: 1247-1247

    • Related Report
      2013 Research-status Report
  • [Journal Article] Enhanced accumulation of atropine in Atropa belladonna transformed by Rac GTPase gene isolated from Scoparia dulcis.2013

    • Author(s)
      Asano, K., Lee, J. B., Yamamura, Y. & Kurosaki, F.
    • Journal Title

      Transgenic Res.

      Volume: 22 Issue: 6 Pages: 1249-1255

    • DOI

      10.1007/s11248-013-9733-4

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Molecular Diversity of Tuliposide A-Converting Enzyme in the Tulip2013

    • Author(s)
      Nomura, T., Tsuchigami, A., Ogita, S. &Kato, Y.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 77 Issue: 5 Pages: 1042-1048

    • DOI

      10.1271/bbb.130021

    • NAID

      10031177594

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Occurrence of hydroxycinnamoylputrescines in xylogenic bamboo suspension cells2013

    • Author(s)
      Nomura, T., Shiozawa, M., Ogita, S. & Kato, Y.
    • Journal Title

      Plant Biotechnology

      Volume: 30 Issue: 5 Pages: 447-453

    • DOI

      10.5511/plantbiotechnology.13.0704a

    • NAID

      130003382493

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Two new b-glucosidases from ethanol-fermenting fungus Mucor circinelloides NBRC 4572: enzyme purification, functional characterization, and molecular cloning of the gene.2013

    • Author(s)
      Kato, Y. Nomura, T. Ogita, S.,Takano, M. & Hoshino K.
    • Journal Title

      Appl. Microbiol. Biotech.

      Volume: 97 Issue: 23 Pages: 10045-10056

    • DOI

      10.1007/s00253-013-5210-5

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Accumulation of sennosides in leaflets, flowers and fruits of Cassia alata.2012

    • Author(s)
      Taniguchi, Y., Tajika, H., Takao, Y., Tatsuo, Y., Yamamura, Y., Kurosaki, F., Fujino, H., Setoyama, T. & Nakazawa, M.
    • Journal Title

      Jpn. J. Pharmacognosy

      Volume: 66 Pages: 77-80

    • NAID

      110009489177

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Jasmonates-induced expression of farnesyl diphosphate synthase gene in Aquilaria microcarpa.2012

    • Author(s)
      Kenmotsu, Y., Yamamura, Y. & Kurosaki, F.
    • Journal Title

      Planta Medica

      Volume: 78 Pages: 1067-1067

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Expression of specific calmodulin genes isolated from tissue cultured cells of Aquilaria microcarpa in response to methyl jasmonate and yeast extract.2012

    • Author(s)
      Kenmotsu, Y., Yamamura, Y. & Kurosaki, F.
    • Journal Title

      In Vitro Cell. Dev. Biol. Plant

      Volume: 48 Pages: 627-631

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] The mutated acetolactate synthase gene from rice as a non-antibiotic selection marker for transformation of bamboo cells,2012

    • Author(s)
      Ogita, S., Kikuchi, N., Nomura T. & Kato, Y.
    • Journal Title

      Am. J. Plant Sci.

      Volume: 3 Pages: 368-372

    • Related Report
      2012 Research-status Report
  • [Journal Article] A novel lactone-forming carboxylesterase: molecular identification of a tuliposide A-converting enzyme in Tulip,2012

    • Author(s)
      Nomura, T., Ogita, S. & Kato, Y.
    • Journal Title

      Plant Physiol.

      Volume: 159 Pages: 565-578

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] A beta-glucosidase activity potentially involved in cell division and wall development of Phyllostachys bamboo suspension cells2012

    • Author(s)
      Ogita, S., Ohki, S., Nomura T. & Kato, Y.
    • Journal Title

      Am. J. Plant Sci.

      Volume: 3 Pages: 1066-1072

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] jasmonate-induced enhancement of expression activity of Am-FaPS-1, a putative farnesyl diphosphate synthase gene from Aquilaria microcarpa.2011

    • Author(s)
      Kenmotsu Y., Ogita S., Katoh Y., Yamamura Y., Takao Y., Tatsuo Y., Fujino H., Kadota S. and Kurosaki F.
    • Journal Title

      J. Nat. Med.

      Volume: 65 Pages: 194-197

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Modification and translocation of Rac/Rop guanosine 5’-triphosphate-binding proteins of Scoparia dulcis in response to stimulation with methyl jasmonate.2011

    • Author(s)
      Mitamura T., Yamamura Y. and Kurosaki F.
    • Journal Title

      Biol. Pharm. Bull.

      Volume: 36 Pages: 845-849

    • NAID

      130000738059

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] A practical protocol for particle bombardment-mediated transformation of Phyllostachys bamboo suspension cells2011

    • Author(s)
      Ogita S., Kikuchi N., Nomura T. and Kato Y.
    • Journal Title

      Plant Biotech.

      Volume: 28 Pages: 43-50

    • NAID

      10028258533

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Characterization of a new (R)-hydroxynitrile lyase from Japanese apricot Prunus mume and cDNA cloning and secretory expression of one of the isozymes in Pichia pastoris2011

    • Author(s)
      Fukuta Y., Nanda S., Kato Y., Yurimoto H., Sakai Y., Komeda H. and Asano Y.
    • Journal Title

      Biosci. Biotech. Biochem.

      Volume: 75 Pages: 214-220

    • NAID

      10027897395

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Asymmetric total synthesis of 6-tuliposide B and its biological activities against Tulip pathogenic fungi2011

    • Author(s)
      Shigetomi K., Omoto S., Kato Y. and Ubukata M.
    • Journal Title

      Biosci. Biotech. Biochem.

      Volume: 75 Pages: 718-722

    • NAID

      10028271584

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Aldoxime dehydratase: probing the heme environment involved in the synthesis of the carbon-nitrogen triple bond2011

    • Author(s)
      PinakoulakiE., Koutsoupakis C., Sawai H., Pavlou A., Kato Y., Asano Y. and Aono S.
    • Journal Title

      J. Phys. Chem.

      Volume: 115 Pages: 13012-13018

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Transcriptional activation of a geranylgeranyl diphosphate synthase gene, GGPPS2, isolated from Scoparia dulcis by the treatment with methyl jasmonate and yeast extract.

    • Author(s)
      Yamamura, Y., Mizuguchi, Y., Taura, F. & Kurosaki, F
    • Journal Title

      Journal of Natural Medicines

      Volume: 68 Issue: 4 Pages: 748-753

    • DOI

      10.1007/s11418-014-0855-7

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Aquilaria microcarpa培養細胞におけるδ-guaiene synthase遺伝子転写活性の誘導2015

    • Author(s)
      廣橋 峻、李 貞範、山村 良美、田浦 太志、黒崎 文也
    • Organizer
      日本薬学会第135年会
    • Place of Presentation
      神戸学院大学、兵庫医療大学
    • Year and Date
      2015-03-25 – 2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] Molecular cloning and functional characterization of an NADPH: cytochrome P450 reductase from a tropical medicinal plant Scoparia dulcis.2013

    • Author(s)
      Yamamura Y., Mabuchi A., Kurosaki F.
    • Organizer
      61st International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA)
    • Place of Presentation
      Muenster, Germany
    • Related Report
      2013 Research-status Report
  • [Presentation] メチルジャスモン酸による植物二次代謝能活性化機構の解明と物質生産への応用2013

    • Author(s)
      黒崎文也,李貞範,山村良美
    • Organizer
      日本生薬学会第60回年会
    • Place of Presentation
      札幌
    • Related Report
      2013 Research-status Report
  • [Presentation] 薬用植物スコパリア由来のNADPH:シトクロームP450 還元酵素の単離と解析2013

    • Author(s)
      馬渕彩香,山村良美,黒崎文也
    • Organizer
      第31回日本植物細胞分子生物学会(札幌)大会
    • Place of Presentation
      札幌
    • Related Report
      2013 Research-status Report
  • [Presentation] 園内に保有する薬用植物の品質評価~第1報キハダ属の品質評価~2012

    • Author(s)
      高尾泰昌,辰尾良秋,山村良美,黒崎文也,藤野廣春,吉田尚利
    • Organizer
      日本生薬学会第59回年会
    • Place of Presentation
      千葉
    • Related Report
      2012 Research-status Report
  • [Presentation] メチルジャスモン酸で誘導されるAquilaria microcarpaのセスキテルペン生合成活性に関わる情報伝達関連遺伝子群のクローニングと発現解析2011

    • Author(s)
      監物慶英, 沢登亮吾, 高尾泰昌, 辰尾良秋, 藤野廣春, 山村良美, 黒崎文也
    • Organizer
      日本生薬学会第58回年会
    • Place of Presentation
      東京
    • Related Report
      2011 Research-status Report
  • [Presentation] 薬用植物Scoparia dulcis由来のチトクロームP450分子種のクローニングと発現解析2011

    • Author(s)
      井上幸枝, 山村良美, 黒崎文也
    • Organizer
      日本生薬学会第58回年会
    • Place of Presentation
      東京
    • Related Report
      2011 Research-status Report
  • [Presentation] coparia dulcis のジテルペン生合成に関与するent-kaurene synthase遺伝子のクローニングと解析2011

    • Author(s)
      江守佑介, 山村良美, 黒崎文也
    • Organizer
      日本生薬学会第58回年会
    • Place of Presentation
      東京
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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