| Project/Area Number |
23590149
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental pharmacy
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
玉田 太郎 日本原子力研究開発機構, 研究員 (50391248)
安達 基泰 日本原子力研究開発機構, 研究員 (60293958)
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| Co-Investigator(Renkei-kenkyūsha) |
佐々木 香織 九州大学, 薬学研究院, 助教 (90464388)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | パーオキシダーゼ / モルヒネ / バイカレイン / ケシ / コガネバナ / 活性酸素種 |
|---|
| Research Abstract |
Hydrogen peroxide, which occurs in plant cells, is known to give serious stress to plant bodies. Many plants construct the systems to detoxify hydrogen peroxide in their bodies, and peroxidases catalyze this detoxification. To precisely understand the mechanism of these detoxifying reactions, I attempted to demonstrate the structures of morphine peroxidase and baicalein peroxidase by X-ray crystallography. As results, I expressed large amounts of recombinant protein in E. coli, whereas they were insoluble and lost the peroxidase activity. Therefore, when I refolded the insoluble and inactive protein, and obtained both peroxidases in the active forms. In addition, we crystallized morphine peroxidase.
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