| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
We have developed a basic technology for a malaria diagnosis system using a cell microarray chip. The chip, with 20,944 microchambers (105 microm width and 50 microm depth) was made from polystyrene. About 130 erythrocytes were accommodated in each microchamber with monolayer formation, there being over 2,700,000 erythrocytes in total on a chip. This detection method promises the potential of a cell microarray chip for the early detection of malaria offering 100 times higher sensitivity than of light microscopy with Giemsa staining.
We are now developing the system based on cell microarray chip technology including erythrocytes isolation column and exclusive fluorescence detector in cooperation with electrical appliance manufacturer. We examined 17 blood samples which were obtained from malaria patients in Hospital using developing diagnosis system. Good correlation was observed between cell microarray chip method and conventional light microscopy.
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