| Project/Area Number |
23590167
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental pharmacy
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| Research Institution | National Institute for Minamata Disease |
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Principal Investigator |
FUJIMURA Masatake 国立水俣病総合研究センター, 基礎研究部, 室長 (20416564)
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| Co-Investigator(Kenkyū-buntansha) |
USUKI Fusako 国立水俣病総合研究センター, 臨床部, 部長 (50185013)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | メチル水銀 / マイクロダイセクション / 小脳顆粒細胞 / 国際情報交換 / フランス / 選択的神経細胞障害 / 中毒学 / 環境系薬学 / 薬学 |
|---|
| Research Abstract |
We isolated granule, Purkinje and molecular layer's neurons in rat cerebellum by using microdissection system and performed real-time PCR analyses for anti-oxidative enzymes. The results suggest that low expression of anti-oxidative enzymes (Mn-SOD, GPx1 and TRxR1) causes CGCs vulnerability to MeHg toxicity.
Next, neurobehavioral analyses revealed that exposure to a low level of MeHg during developmental caused a significant deficit in the motor coordination of rats. The expression of synaptophysin, a marker protein for synaptic formation, significantly decreased in cerebellar granule cells. These results showed that exposure to low-dose MeHg during development induces the dysfunction of motor coordination due to changes of synaptic homeostasis in cerebellar granule cells.
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