Possible novel receptor and its signaling pathway for prostaglandin D2

• Project/Area Number: 23590169
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Medical pharmacy
• Research Institution: Akita University
• Principal Investigator: OYAMADA Hajime 秋田大学, 医学部, 臨床検査技師長 (80375310)
• Co-Investigator(Kenkyū-buntansha): MORITOKI Yuki 秋田大学, 医学部, 講師 (90585522) ; CHIHARA Junichi 蘇生会病院, 名誉理事 (80197615) ; UEKI Shigeharu 秋田大学, 医学部, 准教授 (60361234)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: プロスタグランジンD2 / PGD2受容体 / ケモカイン受容体CCR3 / アレルギー炎症 / DP / CRTH2 / CCR3 / GPER / PGD2 / 気道上皮細胞 / 好酸球

Research Abstract

Prostaglandin D2 (PGD2) has been known to play an important role in allergic inflammation through cell surface receptor CRTH2 and DP1, and also through nuclear receptor PPARg. Our previous study suggested the presence of novel receptor other than these three known receptors for PGD2.
Since we found that PGD2 induced chemokine receptor CCR3 internalization, we studied the possibility that PGD2 act through CCR3. Receptor binding assay and calcium signaling assay using CCR3-transfected cell indicated that PGD2 did not bind/activate CCR3.

Research Products

• [Journal Article] Expression and functional roles of G-protein-coupled estrogen receptor (GPER) in human eosinophils (2014)
  • Author(s): Tamaki M, Konno Y, Kobayashi Y, Takeda M, Itoga M, Moritoki Y, Oyamada H, Kayaba H, Chihara J, Ueki S
  • Journal Title: Immunol Lett Volume: 160(1) Issue: 1 Pages: 72-78
  • DOI: 10.1016/j.imlet.2014.03.013
  • Peer Reviewed
• [Journal Article] Expression and functional roles of G-protein-coupled estrogen receptor (GPER) in human eosinophils. (2014)
  • Author(s): Tamaki M, Konno Y, Kobayashi Y, Takeda M, Itoga M, Moritoki Y, Oyamada H, Kayaba H, Chihara J, Ueki S.
  • Journal Title: Immunology Letters Volume: 160 Issue: 1 Pages: 72-78
  • DOI: 10.1016/j.imlet.2014.03.012
  • Peer Reviewed
• [Journal Article] Adiponectin attenuates human eosinophil adhesion and chemotaxis: implications in allergic inflammation. (2013)
  • Author(s): Yamamoto R, Ueki S, Moritoki Y, Kobayashi Y, Oyamada H, Konno Y, Tamaki M, Itoga M, Takeda M, Ito W, Chihara J.
  • Journal Title: Journal of Asthma Volume: 50 Issue: 8 Pages: 828-835
  • DOI: 10.3109/02770903.2013.816725
  • Peer Reviewed
• [Presentation] Pharmacological PI3kγ inhibitor attenuates eotaxin-induced human eosinophil functions (2014)
  • Author(s): Yukiko Saito,Masahide Takeda,Yasunori Konno,Mami Tamaki,Yuki Moritoki,Shigeharu Ueki,Hajime Oyamada,Makoto Hirokawa
  • Organizer: 第63回日本医学検査学会
  • Place of Presentation: 新潟市
• [Presentation] イムライズ2000による可溶性インターロイキン2受容体(sIL-2R)測定の基礎的検討 (2013)
  • Author(s): 齋藤桃香,鎌田由美子,山内由美子,伊藤祐希,小山田一,廣川誠
  • Organizer: 第37回秋田県医学検査学会
  • Place of Presentation: 由利本荘市
  • Year and Date: 2013-11-03
• [Presentation] 動脈硬化性疾患予防ガイドライン(2012年版)への対応について (2013)
  • Author(s): 井上縁,藤田美好,平澤裕之,高崎貴海,今野裕子,菊地敏子,小山田一,廣川誠
  • Organizer: 第37回秋田県医学検査学会
  • Place of Presentation: 由利本荘市
  • Year and Date: 2013-11-03
• [Presentation] 高感度トロポニンT試薬の評価 (2013)
  • Author(s): 山田千尋,平澤裕之,小熊マリ子,井上縁,今野裕子,菊地敏子,藤田美好,高崎貴海,小山田一,廣川誠
  • Organizer: 第37回秋田県医学検査学会
  • Place of Presentation: 由利本荘市
  • Year and Date: 2013-11-03
• [Presentation] 分子標的薬治療中に重篤な不整脈を発症した慢性骨髄性白血病の一例 (2013)
  • Author(s): 佐藤麻衣子,富谷陽子,高屋のぶ子,山本梨絵,田村麗奈,田中美紀子,齋藤桃香,小山田一,廣川誠
  • Organizer: 第37回秋田県医学検査学会
  • Place of Presentation: 由利本荘市
  • Year and Date: 2013-11-03
• [Presentation] Adiponectin attenuates human eosinophil adhesion and chemotaxis (2013)
  • Author(s): Ueki S, Yamamoto R, Moritoki Y, Kobayashi Y, Oyamada H, Konno Y, Tamaki Y, Itoga M, Takeda M, Chihara J
  • Organizer: 8th Biennial symposium of the international eosinophil society
  • Place of Presentation: Oxford. UK
• [Presentation] 高アルカリ尿が尿蛋白性に及ぼす機器差の検討 (2013)
  • Author(s): 田村明日美,戸島洋子,小熊マリ子,石川千賀子,藤田美好,小山田一,竹田正秀,廣川誠
  • Organizer: 第45回日本臨床検査自動化学会
  • Place of Presentation: 横浜
• [Presentation] Adiponectin attenuates human eosinophil adhesion and chemotaxis. (2013)
  • Author(s): Ueki S, Yamamoto R, Moritoki Y, Kobayashi Y, Oyamada H, Konno Y, Tamaki Y, Itoga M, Takeda M, Chihara J.
  • Organizer: 8th Biennial symposium of the international eosinophil society.
  • Place of Presentation: Oxford. UK.
• [Presentation] 高アルカリ尿が尿蛋白性に及ぼす機器差の検討 (2013)
  • Author(s): 田村明日美，戸島洋子，小熊マリ子，石川千賀子，藤田美好，小山田一，竹田正秀，廣川誠
  • Organizer: 第45回日本臨床検査自動化学会
  • Place of Presentation: 秋田県由利本荘市
• [Presentation] イムライズ2000による可溶性インターロイキン2受容体（ｓIL-2R）測定の基礎的検討． (2013)
  • Author(s): 齋藤桃香，鎌田由美子，山内由美子，伊藤祐希，小山田一，廣川誠
  • Organizer: 第37回秋田県医学検査学会
  • Place of Presentation: 秋田県由利本荘市
• [Presentation] 動脈硬化性疾患予防ガイドライン（2012年版）への対応について (2013)
  • Author(s): 井上 縁，藤田美好，平澤裕之，高崎貴海，今野裕子，菊地敏子，小山田一，廣川誠
  • Organizer: 第37回秋田県医学検査学会
  • Place of Presentation: 秋田県由利本荘市
• [Presentation] 高感度トロポニンＴ試薬の評価 (2013)
  • Author(s): 山田千尋，平澤裕之，小熊マリ子，井上 縁，今野裕子，菊地敏子，藤田美好，高崎貴海，小山田一，廣川 誠
  • Organizer: 第37回秋田県医学検査学会
  • Place of Presentation: 秋田県由利本荘市
• [Presentation] 分子標的薬治療中に重篤な不整脈を発症した慢性骨髄性白血病の一例 (2013)
  • Author(s): 佐藤麻衣子，富谷陽子，高屋のぶ子，山本梨絵，田村麗奈，田中美紀子，齋藤桃香，小山田一，廣川 誠
  • Organizer: 第37回秋田県医学検査学会
  • Place of Presentation: 秋田県由利本荘市
• [Presentation] 好酸球膜受容体CCR3とCRTH2の同時internalizationについての検討 (2012)
  • Author(s): 小林良樹
  • Organizer: 第62回日本アレルギー学会秋期学術大会
  • Place of Presentation: 大阪国際会議場
• [Presentation] Pharmacological PI3k gamma inhibitor attenuates eotaxin-induced human eosinophil functions
  • Author(s): Yukiko Saito, Masahide Takeda, Yasunori Konno, Mami Tamaki, Yuki Moritoki, Shigeharu Ueki, Hajime Oyamada, Makoto Hirokawa
  • Organizer: 第63回日本医学検査学会
  • Place of Presentation: 新潟市