Biomarker strategies using blood microRNA to predict pharmacokinetics

• Project/Area Number: 23590186
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Medical pharmacy
• Research Institution: Kyushu University
• Principal Investigator: HIROTA TAKESHI 九州大学, 薬学研究科(研究院), 助教 (80423573)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
• Keywords: オーダーメード医療 / 薬物トランスポーター / microRNA

Research Abstract

MicroRNA (miRNA) is a non-coding small RNA that regulates gene expression at the translational level by mainly interacting with 3'-untranslated region of target mRNA. It was reported that miR-328 expression influences on breast cancer resistance protein (BCRP) expression in cancer cells. We showed that the methylation patterns of several CpG dinucleotides proximal to identified two C/EBP binding sites in the pre-miR-328 5'-flanking regions were negatively correlated with miR-328 expression and positively associated with BCRP expression in the human placental samples. Recent studies suggested that circulating microRNAs as stable blood-based markers for cancer detection. We analyzed the association between circulating miR-328 expression and the pharmacokinetics of BCRP substrate drug. Circulating miR-328 expression was not significantly associated with the pharmacokinetics.

Research Products

• [Journal Article] Association between DNA methylation in the miR-328 5'-flanking region and inter-individual differences in miR-328 and BCRP expression in human placenta (2013)
  • Author(s): Saito J, Hirota T, Furuta S, Kobayashi D, Takane H, Ieiri I
  • Journal Title: PLoS One Volume: 21;8(8) Issue: 8 Pages: e72906-e72906
  • DOI: 10.1371/journal.pone.0072906
• [Journal Article] Association between DNA methylation in the miR-328 5'-flanking region and inter-individual differences in miR-328 and BCRP expression in human placenta (2013)
  • Author(s): Saito J, Hirota T, Furuta S, Kobayashi D, Takane H, Ieiri I.
  • Journal Title: PLoS One Volume: 8
  • Peer Reviewed
• [Presentation] マイクロRNAによる薬物トランスポーターの発現制御とその機能予測バイオマーカーとしての可能性 (2013)
  • Author(s): 廣田豪, 家入一郎
  • Organizer: 第30回日本TDM学会・学術大会
  • Year and Date: 2013-05-25
• [Presentation] マイクロRNAによる薬物トランスポーターの発現制御とその機能予測バイオマーカーとしての可能性 (2013)
  • Author(s): 廣田豪、家入一郎
  • Organizer: 第30回日本TDM学会・学術大会
  • Place of Presentation: 熊本
• [Presentation] DNAメチル化によるヒトmiR-328発現制御機構の解明 (2012)
  • Author(s): 古田真士, 齋藤順平, 廣田豪, 家入一郎
  • Organizer: 第29回日本薬学会九州支部大会
  • Year and Date: 2012-12-09
• [Presentation] DNA METHYLATION REGULATES miR-328 EXPRESSION WHICH IS ASSOCIATED WITH INTER-INDIVIDUAL DIFFERENCES IN BCRP EXPRESSION IN HUMAN PLACENTA (2012)
  • Author(s): 齋藤順平, 廣田豪, 古田真士, 家入一郎
  • Organizer: 日本薬物動態学会第27回年会
  • Year and Date: 2012-11-22
• [Presentation] ヒトmiR-328発現に個体間変動をもたらすメカニズムの解明 (2012)
  • Author(s): 齋藤順平, 廣田豪, 古田真士, 家入一郎
  • Organizer: 第11回次世代を担う若手ファーマ・バイオフォーラム
  • Place of Presentation: 岩手
  • Year and Date: 2012-09-15
• [Remarks] 九州大学大学院薬学研究院薬物動態学分野Publication
  • URL: http://doutai.phar.kyushu-u.ac.jp/6.publications/publications.html
• [Remarks] 九州大学大学院薬学研究院薬物動態学分野2013年度 Publications
  • URL: http://doutai.phar.kyushu-u.ac.jp/6.publications/2013pub.html