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In vitro approaches to evaluate placental drug transport by using differentiating JEG-3 human choriocarcinoma cells

Research Project

Project/Area Number 23590207
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Medical pharmacy
Research InstitutionOsaka Ohtani University

Principal Investigator

IKEDA Kenji  大阪大谷大学, 薬学部, 講師 (10434812)

Co-Investigator(Renkei-kenkyūsha) UTOGUCHI Naoki  帝京大学, 薬学部, 教授 (80276633)
HIROTANI Yoshihiko  大阪大谷大学, 薬学部, 教授 (00351487)
MYOTOKU Michiaki  大阪大谷大学, 薬学部, 准教授 (30469233)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords胎盤 / モデル / 物質透過 / トロホブラスト / BCRP / DJEG / JEG-3 / 妊娠時薬物療法 / 胎盤関門 / 胎盤透過 / 物質透過モデル / 透過モデル
Research Abstract

Human choriocarcinoma cells have been used as models for studying transcellular drug transport through placental trophoblasts. However, these models allow the transport of low-molecular-weight drugs through intercellular gap junctions. This study aimed to investigate the differentiation patterns of JEG-3 choriocarcinoma cells under different culture conditions and establish the appropriate model of in vitro syncytiotrophoblast drug transport. The differentiation pattern of JEG-3 cultured in CS-C resembled the syncytiotrophoblast-like differentiation signal characterizations in vivo. In conclusion, the syncytiotrophoblast-like models of differentiating JEG-3 cells cultured in CS-C might be appropriate for evaluating drug transport across the placental trophoblast.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (21 results)

All 2014 2013 2012 2011 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (18 results)

  • [Journal Article] Efflux transporter mRNA expression profiles in differentiating JEG-3 human choriocarcinoma cells as a placental transport model2012

    • Author(s)
      池田賢二、山﨑恭平、保米本愛美、山上聡子、小川雅史、中尾絵莉奈、福永悠海、中西剛、宇都口直樹、名德倫明、廣谷芳彦
    • Journal Title

      Pharmazie

      Volume: 67巻 Pages: 88-90

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Efflux transporter mRNA expression profiles in differentiating JEG-3 human choriocarcinoma cells as a placental transport model.2012

    • Author(s)
      Kenji IKEDA
    • Journal Title

      Pharmazie

      Volume: 67 Pages: 86-90

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] In Vitro Approaches to Evaluate Placental Drug Transport by Using Differentiating JEG-3 Human Choriocarcinoma Cells2011

    • Author(s)
      池田賢二、宇都口直樹、筒居秀伸、山上聡子、保米本愛美、中尾絵莉奈、福永悠海、山﨑恭平、名德倫明、廣谷芳彦
    • Journal Title

      Basic Clin. Pharmacol. Toxicol

      Volume: 108巻 Pages: 138-145

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] In vitro胎盤薬物透過評価系細胞層におけるリアルタイム経上皮電気抵抗値測定2014

    • Author(s)
      池田賢二
    • Organizer
      第31回日本TDM学会
    • Place of Presentation
      ソラシティカンファレンスセンター(東京)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Effect of Breast Cancer Resistance Protein on Cimetidine Transport across the Placental model using Differentiating Human Choriocarcinoma JEG-3 Cells2013

    • Author(s)
      池田賢二
    • Organizer
      13th International Congress of Therapeutic Drug Monitoring & Clinical Toxicology (IATDMCT)
    • Place of Presentation
      Grand America Hotel, Salt Lake City, USA
    • Year and Date
      2013-09-26
    • Related Report
      2013 Final Research Report
  • [Presentation] Effect of Breast Cancer Resistance Protein on Cimetidine Transport across the Placental model using Differentiating Human Choriocarcinoma JEG-3 Cells2013

    • Author(s)
      池田賢二
    • Organizer
      13th International Congress of Therapeutic Drug Monitoring & Clinical Toxicology
    • Place of Presentation
      Grand America Hotel, Salt Lake City, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] ヒト由来がん細胞株の増殖率に及ぼす各種培養条件の影響2012

    • Author(s)
      小宮山水智、池田賢二, 他
    • Organizer
      第22回日本医療薬学会年会
    • Place of Presentation
      新潟朱鷺メッセ
    • Year and Date
      2012-10-28
    • Related Report
      2013 Final Research Report
  • [Presentation] 新規in vitro胎盤薬物透過評価モデルにおける物質透過機能解析2012

    • Author(s)
      寺尾佳子、池田賢二, 他
    • Organizer
      第62回日本薬学会近畿支部総会・大会
    • Place of Presentation
      武庫川女子大学・薬学部
    • Year and Date
      2012-10-20
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] 医薬品の胎児移行性評価に適う細胞層モデルの検討2012

    • Author(s)
      池田賢二
    • Organizer
      日本薬学会第132年会シンポジウム(妊娠時薬物療法に関わる基礎と臨床の情報共有機会拡大に関する提言)
    • Place of Presentation
      北海道大学
    • Year and Date
      2012-03-31
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒト由来がん細胞株の増殖率に及ぼす各種培養条件の影響2012

    • Author(s)
      小宮山 水智
    • Organizer
      第22回日本医療薬学会年会
    • Place of Presentation
      新潟 朱鷺メッセ
    • Related Report
      2012 Research-status Report
  • [Presentation] 医薬品医療機器総合機構発出医薬品情報の周知方法に関する検討2011

    • Author(s)
      福永悠海、池田賢二, 他
    • Organizer
      第21回日本医療薬学会年会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2011-10-01
    • Related Report
      2013 Final Research Report
  • [Presentation] 分化JEG-3細胞株によるIn vitro胎盤薬物透過評価モデルを用いたSSRIおよび睡眠剤の栄養膜透過に関する検討2011

    • Author(s)
      山﨑恭平、池田賢二, 他
    • Organizer
      第21回日本医療薬学会年会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2011-10-01
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒト絨毛癌細胞株BeWoとJEG-3のBreast Cancer Resistance Protein遺伝子多型判定について2011

    • Author(s)
      保米本愛美、池田賢二, 他
    • Organizer
      第21回日本医療薬学会年会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2011-10-01
    • Related Report
      2013 Final Research Report
  • [Presentation] 分化させたヒト絨毛癌細胞株JEG-3のMultidrug associated protein発現誘導の比較検討2011

    • Author(s)
      中尾絵莉奈、池田賢二, 他
    • Organizer
      第21回日本医療薬学会年会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2011-10-01
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒト絨毛癌細胞株JEG-3及びBeWo細胞層の胎盤薬物透過モデルにおける有用性の比較2011

    • Author(s)
      山上聡子、池田賢二, 他
    • Organizer
      第21回日本医療薬学会年会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2011-10-01
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒト絨毛癌細胞株JEG-3及びBeWo細胞層の胎盤薬物透過モデルにおける有用性の比較

    • Author(s)
      山上 聡子
    • Organizer
      第21回日本医療薬学会年会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2011 Research-status Report
  • [Presentation] 分化させたヒト絨毛癌細胞株JEG-3のMultidrug associated protein発現誘導の比較検討

    • Author(s)
      中尾 絵莉奈
    • Organizer
      第21回日本医療薬学会年会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2011 Research-status Report
  • [Presentation] ヒト絨毛癌細胞株 BeWo とJEG-3のBreast Cancer Resistance Protein 遺伝子多型判定について

    • Author(s)
      保米本 愛美
    • Organizer
      第21回日本医療薬学会年会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2011 Research-status Report
  • [Presentation] 分化JEG-3細胞株によるIn vitro 胎盤薬物透過評価モデルを用いたSSRIおよび睡眠剤の栄養膜透過に関する検討

    • Author(s)
      山崎 恭平
    • Organizer
      第21回日本医療薬学会年会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2011 Research-status Report
  • [Presentation] 医薬品医療機器総合機構発出医薬品情報の周知方法に関する検討

    • Author(s)
      福永 悠海
    • Organizer
      第21回日本医療薬学会年会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2011 Research-status Report
  • [Presentation] 妊娠時薬物療法に関わる基礎と臨床の情報共有機会拡大に関する提言-医薬品の胎児移行性評価に適う細胞層モデルの検討-

    • Author(s)
      池田 賢二
    • Organizer
      第132回日本薬学会年会シンポジウム42(招待講演)
    • Place of Presentation
      北海道大学
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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