Studies on controlling ventral migration of nucleus ambiguus motoneurons associated with Reelin-signaling and neurotrophic factors

• Project/Area Number: 23590218
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Kobe University
• Principal Investigator: SETSU Tomiyoshi 神戸大学, 大学院医学研究科, 技術専門員 (30403231)
• Co-Investigator(Renkei-kenkyūsha): TERASHIMA Toshio 神戸大学, 大学院医学研究科, 教授 (20101892)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: リーリン / Dab1 / 顔面神経核 / 疑核 / マウス / 細胞移動 / 疑核ニューロン

Research Abstract

To clarify the functional role of the Reelin-signaling pathway on the ventral migration of branchiogenic motoneurons, we compared the distribution of facial and ambiguus motoneurons between reeler and Dab1-deficient yotari mice using ChAT-immunohistochemistry and retrograde labeling technique. The migration of the facial nucleus neurons is affected severely in yotari but slightly in reeler, whereas the ambiguus nucleus is equally affected in these two mutants. The present study suggests that ventral migration of the nucleus ambiguus motoneurons is Reelin-dependent, whereas the migration of facial motoneurons is time controlled by some other signaling components (e.g. some neurotrophic factors expressed by migrating motoneruons) through dab1 during development. In conclution, Reelin and Dab1 play some different roles during migration and/or post-migration rearrangements of facial and ambiguus motoneurons.

Research Products

• [Presentation] リーリンシグナル伝達系の変異マウスreelerおよびyotariの顔面神経核と疑核の細胞構築異常 (2014)
  • Author(s): 薛富義, 寺島俊雄
  • Organizer: 第119回日本解剖学会総会
  • Place of Presentation: 下野市
• [Presentation] リーリンシグナル伝達系の変異マウス reeler および yotari の顔面神経核と疑核の細胞構築異常 (2014)
  • Author(s): 薛 富義，寺島俊雄
  • Organizer: 第119回日本解剖学会
  • Place of Presentation: 自治医大（栃木）
• [Remarks]
  • URL: http://www.med.kobe-u.ac.jp/anato1/Anat1_home.html
• [Remarks] 神戸大学医学研究科 生理学細胞生物学講座 神経発生学分野オリジナルホームページ
  • URL: http://www.med.kobe-u.ac.jp/anato1/Anat1_home.html