| Project/Area Number |
23590221
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Nara Medical University |
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Principal Investigator |
WANAKA Akio 奈良県立医科大学, 医学部, 教授 (90210989)
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| Co-Investigator(Kenkyū-buntansha) |
TATSUMI Kouko 奈良県立医科大学, 医学部, 准教授 (90208033)
OKUDA Hiroaki 奈良県立医科大学, 医学部, 助教 (40453162)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | OASIS / コンドロイチン硫酸 / 神経再生 / アストロサイト / 転写調節 / ノックアウトマウス / 脳損傷 / 糖鎖修飾 / C6ST-1 / 転写因子 |
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| Research Abstract |
We found that chondroitin sulfate proteoglycan expression is reduced in the OASIS-kockout mouse as compared to the wild type mouse. We screened that target genes of OASIS transcription in the CSPG production pathway and found that C6ST-1, an enzyme that transfers sulfate moieties to CSPG, was a potential candidate. By artificial transcription assay system, we examined whether C6ST-1 transcription is regulated by OASIS or not. The first intron of the C6ST-1 genome contained several CRE (OASIS binding site) and deletion of those CREs reduced C6ST-1 transcription. Taken together, we concluded that OASIS inhibits neuronal regeneration by regulating C6ST-1 activities in the injured central nervous system.
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