Molecular mechanism of cellular interaction between placode and neural crest

• Project/Area Number: 23590224
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Hyogo Medical University
• Principal Investigator: IKEDA Keiko 兵庫医科大学, 医学部, 教授 (10265241)
• Research Collaborator: KAWAKAMI Kiyoshi 自治医科大学, 医学部, 教授 (10161283)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: プラコード / 神経堤 / 細胞間相互作用 / 発生 / Six 遺伝子 / 外胚葉ボーダー細胞 / 全胚培養 / マウス / 電気穿孔法 / 遺伝子改変 / ROSA / Six遺伝子群 / エンハンサー / Cre / レーザーキャプチャー

Research Abstract

The cranial peripheral nervous system is unique in the sense that it is derived from both ectodermal placodes and neural crest cells (NCCs). The interdependent relationship exists between placodes and NCCs all through development. Considering obvious importance of understanding the interactions between placodes and NCCs, it is not fully uncovered about the ligands and receptors governing their interactions, especially in the mammals. The aim of this research was to identify molecules for the interaction. We have already reported that transcription factor encoding genes, Six1 and Six4 expressed solely in the placodes, but not in the NCCs. Knockout mouse embryos targeting these genes showed defects not only placodal, but also NCCs derived organs. Using the gene targeting mice and wild type one, here we searched candidate molecules essential for the interaction between placodes and NCCs. We also examined the effects of inhibiting the expression of candidate molecules during development.

Research Products

• [Journal Article] Distinct neurological disorders with ATP1A3 mutations. (2014)
  • Author(s): Heinzen, E.L.*, Arzimanoglou, A., Brashear, A., Clapcote, S.J., Gurrieri, F., Goldstein, D.B., Johannesson, S.H., Mikati, M.A., Neville, B., Nicole, S., Ozelius, L.J., Poulsen, H., Schyns, T., Sweadner, K.J., van den Maagdenberg, A., Vilsen, B., the ATP1A3 Working Group (included Ikeda, K.).
  • Journal Title: Lancet Neurol. Volume: 13 Issue: 5 Pages: 503-514
  • DOI: 10.1016/s1474-4422(14)70011-0
  • Peer Reviewed / Open Access
• [Journal Article] Enhanced inhibitory neurotransmission in the cerebellar cortex of Atp1a3-deficient heterozygous mice (2013)
  • Author(s): Ikeda, K., Satake, S., Onaka, T., Sugimoto, H., Takeda, N., Imoto, K., Kawakami, K.
  • Journal Title: J. Physiol Volume: 591 (IF=4.718) Pages: 3433-3449
  • Peer Reviewed
• [Journal Article] 遺伝子改変マウス(Atp1a3+/-)を使った家族性ジストニアパーキンソニズムの病態解明 (2013)
  • Author(s): 池田啓子
  • Journal Title: 加藤記念難病助成基金研究報告集 Volume: 26 Pages: 9-21
• [Journal Article] Enhanced inhibitory neurotransmission in the cerebellar cortex of Atp1a3-deficient heterozygous mice. (2013)
  • Author(s): Ikeda K., Satake S., Onaka T., Sugimoto H., Takeda N., Imoto K., Kawakami K.
  • Journal Title: Journal of Physiology Volume: 591 Issue: 13 Pages: 3433-3449
  • DOI: 10.1113/jphysiol.2012.247817
  • Peer Reviewed
• [Journal Article] 遺伝子改変マウス(Atp1a3+/-)を使った家族性ジストニアパーキンソニズムの病態解明. (2013)
  • Author(s): 池田啓子*
  • Journal Title: 加藤記念難病助成基金 Volume: 26 Pages: 9-21
• [Journal Article] Regulation of Six1 expression by evolutionarily conserved enhancers in tetrapods (2012)
  • Author(s): Sato, S., Ikeda, K., Shioi, G., Nakao K., Yajima, H., Kawakami, K
  • Journal Title: Dev. Biol Volume: 368 (IF= 4.069) Pages: 95-108
  • Peer Reviewed
• [Journal Article] Postsynaptic mechanisms of CO_2 responses in parafacial respiratory neurons of newborn rats (2012)
  • Author(s): Onimaru, H., Ikeda, K., Kawakami, K
  • Journal Title: J. Physiol Volume: 590 (IF=4.718) Pages: 1615-1624
  • Peer Reviewed
• [Journal Article] Relationship between the distribution of the paired-like homeobox gene (Phox2b) expressing cells and blood vessels in the parafacial region of the ventral medulla of neonatal rats (2012)
  • Author(s): Onimaru, H., Ikeda, K., Kawakami, K
  • Journal Title: Neuroscience Volume: 212 (IF=3.38) Pages: 131-139
  • Peer Reviewed
• [Journal Article] Two distinct neurogenesis in olfactory epithelium (2012)
  • Author(s): Ikeda, K.
  • Journal Title: Jpn. J. Taste Smell Res Volume: 19(1) Pages: 63-69
  • Peer Reviewed
• [Journal Article] 嗅上皮における2種類のNeurogenesis (2012)
  • Author(s): 池田啓子
  • Journal Title: 日本味と匂学会誌 Volume: 19 Pages: 63-69
  • Peer Reviewed
• [Journal Article] Regulation of Six1 expression by evolutionarily conserved enhancers in tetrapods (2012)
  • Author(s): Sato S, Ikeda K, Shioi G, Nakao K, Yajima H, Kawakami K.
  • Journal Title: Dev Biol Volume: 368 Issue: 1 Pages: 95-108
  • DOI: 10.1016/j.ydbio.2012.05.023
  • Peer Reviewed
• [Journal Article] Relationship between the distribution of Phox2b expressing cells and blood vessels in the parafacial region of the ventral medulla of neonatal rats (2012)
  • Author(s): Onimaru, H., Ikeda, K., Kawakami, K.
  • Journal Title: Neuroscience Volume: (印刷中) Pages: 131-139
  • DOI: 10.1016/j.neuroscience.2012.03.037
  • Peer Reviewed
• [Journal Article] Postsynaptic mechanisms of CO2 responses in parafacial pre-inspiratory neurons of newborn rats (2012)
  • Author(s): Onimaru, H., Ikeda, K., Kawakami, K.
  • Journal Title: J.Phys. Volume: (印刷中) Issue: 7 Pages: 1615-1624
  • DOI: 10.1113/jphysiol.2011.222687
  • Peer Reviewed
• [Journal Article] Postsynaptic mechanisms of CO2 responses in parafacial respiratory neurons of newborn rats. (2012)
  • Author(s): Onimaru H, Ikeda K, Kawakami K.
  • Journal Title: J Physiol Volume: 590(Pt 7) Pages: 1615-1624
  • Peer Reviewed
• [Journal Article] Relationship between the distribution of Phox2b expressing cells and blood vessels in the parafacial region of the ventral medulla of neonatal rats (2012)
  • Author(s): Onimaru H, Ikeda K, Kawakami K.
  • Journal Title: Neuroscience Volume: in press
  • Peer Reviewed
• [Journal Article] Two distinct neurogenesis in olfactory epithelium. (Review) (2012)
  • Author(s): Ikeda K.
  • Journal Title: Jap J Taste Smell Res Volume: in press
• [Journal Article] Development of gustatory papillae in the absence of Six1 and Six4 (2011)
  • Author(s): Suzuki, Y., Ikeda, K., Kawakami, K
  • Journal Title: J. Anat Volume: 219 (IF= 2.37) Pages: 710-721
  • NAID: 110009441199
  • Peer Reviewed
• [Journal Article] Development of gustatory papillae in the absence of Six1 and Six4. (2011)
  • Author(s): Suzuki Y, Ikeda K, Kawakami K.
  • Journal Title: J Anat Volume: 219 Issue: 6 Pages: 710-721
  • DOI: 10.1111/j.1469-7580.2011.01435.x
  • NAID: 110009441199
  • Peer Reviewed
• [Presentation] モデルマウスを使った小児交代性片麻痺の病態分子基盤の解明 (2013)
  • Author(s): 池田啓子
  • Organizer: 第66回兵庫県医師会設立記念式典受賞記念講演
  • Place of Presentation: 兵庫県医師会館,兵庫 (特別講演)
  • Year and Date: 2013-11-10
• [Presentation] Toward the understanding sodium pump disease (2013)
  • Author(s): Kawakami, K., Sugimoto H., Ikeda, K
  • Organizer: 10th Nikko Symposium
  • Place of Presentation: Tochigi, Japan (シンポジウム, Abstract book p.14)
  • Year and Date: 2013-10-17
• [Presentation] Naポンプの最近の知見〜周産期におけるNaポンプの役割〜 (2013)
  • Author(s): 池田啓子
  • Organizer: 第6回福井生殖生物学・医学研究会
  • Place of Presentation: 福井 (特別講演)
  • Year and Date: 2013-08-30
• [Presentation] Atp1a3-deficient heterozygous mice show shorter stride and fall latency in hanging box in chronic stress condition (2013)
  • Author(s): Sugimoto, H., Ikeda, K., Kawakami, K
  • Organizer: 2nd Symposium on ATP1A3 in disease
  • Place of Presentation: Rome, Italy (poster)
• [Presentation] Increased inhibitory neurotransmission in the cerebellum of the Atp1a3-deficient heterozygous mice (2013)
  • Author(s): Ikeda, K., Satake, S., Kawakami, K
  • Organizer: 2nd Symposium on ATP1A3 in disease
  • Place of Presentation: Rome, Italy (poster)
• [Presentation] Expression of Six genes in the mouse early development of mouse and the role in cranial gangliogenesis (2013)
  • Author(s): Ikeda, K, Sato, S., Kawakami, K
  • Organizer: Gordon Research Conference : Neural Crest & Cranial Placodes
  • Place of Presentation: Stonehill College in Easton MA, United States (poster)
• [Presentation] Role of Six1 in evolution of vertebrate primary sensory system (2013)
  • Author(s): Kawakami, K., Yajima, H., Suzuki, M., Ochi,H., Ikeda, K., Sato, S., Ogino, H., Ueno, N
  • Organizer: 46^<th> Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: Matsue, Japan (Program Book : p.35)
• [Presentation] Atp1a3+/-マウス小脳皮質において登上線維伝達物質のシナプス外拡散は強く抑制されている (2013)
  • Author(s): 佐竹伸一郎、池田啓子、川上潔、井本敬二
  • Organizer: 第36回日本神経科学大会Neuro2013
  • Place of Presentation: 国立京都会館,京都(Abstract book p.277, (poster, P2-2-33)
• [Presentation] Expression of Six genes in the mouse early development of mouse and the role in cranial gangliogenesis. (2013)
  • Author(s): Ikeda, K.*, Sato, S., Kawakami, K.
  • Organizer: Gordon Research Conference: Neural Crest & Cranial Placodes
  • Place of Presentation: Stonehill College in Easton MA United States
• [Presentation] Expression of Six genes in the mouse early development of mouse and the role in cranial gangliogenesis. (2013)
  • Author(s): Ikeda, K.*, Satake, S., Kawakami, K.
  • Organizer: 2nd Symposium on ATP1A3 in disease
  • Place of Presentation: Rome, Italy
• [Presentation] Atp1a3-deficient heterozygous mice show shorter stride and fall latency in hanging box in chronic stress condition. (2013)
  • Author(s): Sugimoto, H., Ikeda, K., Kawakami, K.*
  • Organizer: 2nd Symposium on ATP1A3 in disease
  • Place of Presentation: Rome, Italy
• [Presentation] Atp1a3+/-マウス小脳皮質において登上線維伝達物質のシナプス外拡散は強く抑制されている. (2013)
  • Author(s): 佐竹伸一郎*、池田啓子、川上潔、井本敬二
  • Organizer: 第36回日本神経科学大会Neuro2013
  • Place of Presentation: 京都、京都
• [Presentation] Role of Six1 in evolution of vertebrate primary sensory system. (2013)
  • Author(s): Kawakami, K.*, Yajima, H., Suzuki, M., Ochi,H., Ikeda, K., Sato, S., Ogino, H., Ueno, N.
  • Organizer: 46th Annual Meeting for the Japanese Society of Developmental Biologists.
  • Place of Presentation: 島根、松江
• [Presentation] 頭部感覚器発生を司る遺伝子カスケード (2012)
  • Author(s): 池田啓子
  • Organizer: 兵庫医科大学医学会学術講演会
  • Place of Presentation: 兵庫 (特別講演)
  • Year and Date: 2012-01-23
• [Presentation] Altered inhibitory neurotransmission in the cerebellum DYT12 model mice (2012)
  • Author(s): Ikeda, K., Satake, S., Onaka, T., Takeda, N., Yamada, G., Imoto, K., Kawakami, K
  • Organizer: Neuroscience 2012 (第25回日本神経科学会)
  • Place of Presentation: 名古屋
• [Presentation] Development of gustatory papillae in the absence of Six1 and Six4 (2012)
  • Author(s): Ikeda, K., Suzuki, Y., Kawakami, K
  • Organizer: Society for Developmental Biology 71st Annual Meeting
  • Place of Presentation: Motreal, Canada (poster)
• [Presentation] Key regulator for developmental and evolutionary switch from Rohon-Beard cells to dorsal root ganglia (2012)
  • Author(s): Yajima, H., Suzuki, M., Ochi, H., Ikeda, K., Sato, S., Ogino, H., Ueno, N., Kawakami, K
  • Organizer: Society for Developmental Biology 71st Annual Meeting
  • Place of Presentation: Motreal, Canada (poster)
• [Presentation] Six1 expression is regulated by evolutionarily conserved enhancers (2012)
  • Author(s): Sato, S., Ikeda, K., Shioi, G., Nakao, K., Yajima, H., Kawakami, K
  • Organizer: Society for Developmental Biology 71st Annual Meeting
  • Place of Presentation: Motreal, Canada (poster)
• [Presentation] Developmental defects of gustatory papillae in Six gene-knockout mice (2012)
  • Author(s): Ikeda, K., Suzuki, Y., Kawakami, K
  • Organizer: Joint meeting of the 45th Annual meeting of JSDB and the 64th Annual meeting of JSCB
  • Place of Presentation: 神戸
• [Presentation] Conservation and diversity of Six1 gene enhancers in chordate (2012)
  • Author(s): Sato, S., Ikeda, K., Shioi, G., Nakao K., Yajima, H., Aizawa, S., Kawakami, K
  • Organizer: Joint meeting of the 45th Annual meeting of JSDB and the 64th Annual meeting of JSCB
  • Place of Presentation: 神戸
• [Presentation] Developmental defects of gustatory papillae in Six gene-knockout mice. (2012)
  • Author(s): Keiko Ikeda, Yuko Suzuki, Kiyoshi Kawakami
  • Organizer: Joint Meeting of the 45th Annual Meeting of JSDB and the 64th Annual Meeting of JSCB
  • Place of Presentation: 神戸
• [Presentation] Conservation and diversity of Six1 gene enhancers in chordate. (2012)
  • Author(s): Shigeru Sato, Keiko Ikeda, Go Shioi, Kazuki Nakao, Shinichi Aizawa, Hiroshi Yajima, Kiyoshi Kawakami
  • Organizer: Joint Meeting of the 45th Annual Meeting of JSDB and the 64th Annual Meeting of JSCB
  • Place of Presentation: 神戸
• [Presentation] Development of gustatory papillae in the absence of Six1 and Six4. (2012)
  • Author(s): Keiko Ikeda, Yuko Suzuki, Kiyoshi Kawakami
  • Organizer: Society for developmental Biology 71st Annual Meeting
  • Place of Presentation: Montreal, Canada
• [Presentation] Key regulator for developmental and evolutionary switch from Rohon-Beard cells to dorsal root ganglia. (2012)
  • Author(s): Yajima H, Suzuki M, Ochi H, Ikeda K, Sato S, Ogino H, Ueno N, Kawakami K
  • Organizer: Society for developmental Biology 71st Annual Meeting
  • Place of Presentation: Montreal, Canada
• [Presentation] Six1 expression is regulated by evolutionarily conserved enhancers. (2012)
  • Author(s): Sato S, Ikeda K, Shioi G, Nakao K, Yajima H, Kawakami K
  • Organizer: Society for developmental Biology 71st Annual Meeting
  • Place of Presentation: Montreal, Canada
• [Presentation] Altered inhibitory neurotransmission in the cerebellum of DYT12 model mice. (2012)
  • Author(s): Keiko Ikeda, Shin'Ichiro Satake, Tatsushi Onaka, Naoki Takeda, Gen Yamada, Keiji Imoto, Kiyoshi Kawakami
  • Organizer: Neuroscience 2012 第35回日本神経科学会
  • Place of Presentation: 名古屋
• [Presentation] 嗅上皮発生段階におこるneurogenesisを司る遺伝子カスケード (2011)
  • Author(s): 池田啓子
  • Organizer: 日本味と匂学会第45回大会
  • Place of Presentation: 金沢(シンポジウム)
• [Presentation] Six1/4二重欠損マウスにおける舌乳頭の発生 (2011)
  • Author(s): 鈴木裕子,池田啓子,川上潔
  • Organizer: 日本味と匂学会第45回大会
  • Place of Presentation: 金沢(poster)
• [Presentation] Expression pattern of Six genes in the mouse early embryos (2011)
  • Author(s): Ikeda, K., Sato, S., Yajima, H., Kawakami, K
  • Organizer: 第44回日本発生生物学会年会
  • Place of Presentation: 沖縄宜野湾 (poster)
• [Presentation] Heterochronic shift of Six1 expression drives evolutionary transition of vertebrate primary sensory neurons (2011)
  • Author(s): Yajima, H., Suzuki, M., Ochi, H., Ikeda, K., Sato, S., Ogino, H., Ueno, N., Kawakami, K
  • Organizer: 第44回日本発生生物学会年会
  • Place of Presentation: 沖縄宜野湾(poster)
• [Presentation] Expression pattern of Six genes in the mouse early embryos. (2011)
  • Author(s): Ikeda K, Sato S, Yajima H, Kawakami K.
  • Organizer: 第44回日本発生生物学会年会
  • Place of Presentation: 沖縄宜野湾
• [Presentation] Heterochronic shift of Six1 expression drives evolutionary transition of vertebrate primary sensory neurons. (2011)
  • Author(s): Yajima H, Suzuki M, Ochi H, Ikeda K, Sato S, Ogino H, Ueno N, Kawakami K.
  • Organizer: 第44回日本発生生物学会年会
  • Place of Presentation: 沖縄宜野湾
• [Presentation] 嗅上皮発生段階におこるNeurogenesisを司る遺伝子カスケード. (2011)
  • Author(s): 池田啓子
  • Organizer: 日本味と匂学会第45回大会（シンポジウム）
  • Place of Presentation: 金沢
• [Presentation] Six1/4二重欠損マウスにおける舌乳頭の発生. (2011)
  • Author(s): 鈴木裕子、池田啓子、川上潔
  • Organizer: 日本味と匂学会第45回大会
  • Place of Presentation: 金沢
• [Presentation] Toward the understanding of Na-pump disease.
  • Author(s): Kawakami, K., Ikeda, K., Sugimoto, H.
  • Organizer: The 10th Nikko International Symposium 2013
  • Place of Presentation: 栃木、下野
• [Presentation] Naポンプの最近の知見～周産期におけるNaポンプの役割～
  • Author(s): 池田啓子
  • Organizer: 第6回福井生殖生物学・医学研究会
  • Place of Presentation: 福井、福井
• [Presentation] モデルマウスを使った小児交代性片麻痺の病態分子基盤の解明
  • Author(s): 池田啓子*
  • Organizer: 第66回兵庫県医師会
  • Place of Presentation: 兵庫、神戸
• [Remarks] 研究紹介
  • URL: http://www.hyo-med.ac.jp/faculty/course/biology.html