Evolutionary diversity of podocyte slit diaphragm in metazoans

• Project/Area Number: 23590226
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Juntendo University
• Principal Investigator: ICHIMURA Koichiro 順天堂大学, 医学部, 准教授 (10343485)
• Co-Investigator(Kenkyū-buntansha): HIDETAKE Kurihara 順天堂大学, 医学部, 先任准教授 (80311976)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 比較解剖学 / 比較細胞組織学 / 腎臓 / 糸球体 / 足細胞 / スリット膜 / 電子顕微鏡 / 炎細胞 / ネフリン / プラナリア / 糸球体上皮細胞 / 足突起 / 体腔 / 中皮細胞

Research Abstract

Slit diaphragm (SD) is a highly specialized intercellular junction between podocyte foot processes and plays a crucial role in the formation of the filtration barrier. In this study, we examined the developmental localization of Nephrin, an essential component of SD, in the pronephric glomerulus of zebrafish and medaka. In the mature glomerulus of both fish, Nephrin is localized along the glomerular basement membrane like in mammals, indicating that Nephrin is localized at the SD. Nephrin was localized along the cell surface of immature podocytes, but as different localization patterns. In zebrafish, Nephrin signal bordered the lateral membrane of podocytes, which were columnar in shape as are in rat immature podocytes. However, in medaka immature podocytes, Nephrin was localized in a punctate pattern among podocyte cell bodies. These findings suggest that Nephrin is needed to be integrated to the membrane before the formation of SD and then move to the proper site to form SD.

Research Products

• [Journal Article] Podocalyxin regulates pronephric glomerular development in zebrafish. (2014)
  • Author(s): Ichimura K, Nakamura T, Powell R, Kurihara H, Sakai T, Obara T.
  • Journal Title: Physiological Report Volume: 1 Issue: 3
  • DOI: 10.1002/phy2.74
  • Peer Reviewed
• [Journal Article] Podocalyxin regulates pronephric glomerular development in zebrafish (2013)
  • Author(s): Ichimur a K, Fukuyo Y, Nakamura T, Powell R, Sakai T, Obara T
  • Journal Title: Physiol Rep Volume: 1(3)
  • Peer Reviewed
• [Journal Article] Developmental localization of Nephrin in zebrafish and medaka pronephric glomerulus (2013)
  • Author(s): Ichimur a K, Fukuyo Y, Nakamura T, Powell R, Sakai T, Janknecht R, Obara T
  • Journal Title: J Histochem Cytochem Volume: 61(4) Pages: 313-24
  • Peer Reviewed
• [Journal Article] Medaka fish, Oryzias latipes, as a model for human obesity-related glomerulopathy (2013)
  • Author(s): Ichimur a K, Kawashima Y, Nakamura T, Powell R, Hidoh Y, Terai S, Sakaida I, Kodera Y, Tsuji T, Ma JX, Sakai T, Matsumoto H, Obara T
  • Journal Title: Biochem Biophys Res Commun Volume: 431(4) Pages: 712-7
  • Peer Reviewed
• [Journal Article] Elongation of in vitro cultured ovarian follicles in the mosquito, Culex pipiens pallens (2013)
  • Author(s): Uchida K, Ichimur a K
  • Journal Title: J Insect Physiol Volume: 59(1) Pages: 38-45
  • Peer Reviewed
• [Journal Article] Medaka fish, Oryzias latipes, as a model for human obesity-related glomerulopathy (2013)
  • Author(s): Ichimura K, Kawashima Y, Nakamura T, Powell R, Hidoh Y, Terai S, Sakaida l, Kodera Y, T suji T, Ma JX, Sakai T, Matsumoto H, Obara T
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 431 Issue: 4 Pages: 712-717
  • DOI: 10.1016/j.bbrc.2013.01.053
  • Peer Reviewed
• [Journal Article] Developmental localization of Nephrin in zebrafish and medaka pronephric glomerulus (2013)
  • Author(s): Ichimura K, Fukuyo Y, Nakamura T, Powell R, Sakai T, Janknecht R, Obara T
  • Journal Title: Journal of Histochemistry Cytochemistry Volume: 61(4) Issue: 4 Pages: 313-24
  • DOI: 10.1369/0022155413477115
  • Peer Reviewed
• [Journal Article] Elongation of in vitro cultured ovarian follicles in the mosquito, Culex pipiens pallens (2013)
  • Author(s): Uchida K, Ichimura K
  • Journal Title: Journal of Insect Physiology Volume: 59(1) Issue: 1 Pages: 38-45
  • DOI: 10.1016/j.jinsphys.2012.09.003
  • Peer Reviewed
• [Journal Article] A comparative analysis of glomerulus development in the pronephros of medaka and zebrafish (2012)
  • Author(s): Ichimur a K, Bubenshchikova E, Powell R, Fukuyo Y, Nakamura T, Tran U, Oda S, Tanaka M, Wessely O, Kurihara H, Sakai T, Obara T
  • Journal Title: PLoS One Volume: 7(9)
  • Peer Reviewed
• [Journal Article] Structural disorganization of pronephric glomerulus in zebrafish mpp5a/nagie oko mutant (2012)
  • Author(s): Ichimur a K, Fukuyo Y, Nakamura T, Powell R, Sakai T, Obara T
  • Journal Title: Dev Dyn Volume: 241(12) Pages: 1922-32
  • Peer Reviewed
• [Journal Article] Wtip and Vangl2 are required for mitotic spindle orientation and cloaca morphogenesis (2012)
  • Author(s): Bubenshchikova E, Ichimur a K, Fukuyo Y, Powell R, Hsu C, Morrical SO, Sedor JR, Sakai T, Obara T
  • Journal Title: Biol Open Volume: 1 Pages: 588-596
  • Peer Reviewed
• [Journal Article] Structural disorganization of pronephric glomerulus in zebrafish mpp5a/nagie oko mutant (2012)
  • Author(s): Ichimura K, Fukuyo Y, Nakamura T, Powell R, Sakai T, Obara T
  • Journal Title: Developmental Dynamics Volume: 241 Issue: 12 Pages: 1922-1932
  • DOI: 10.1002/dvdy.23877
  • Peer Reviewed
• [Journal Article] A comparative analysis of glomerulus development in the pronephros of medaka and zebrafish (2012)
  • Author(s): Ichimura K, Bubenshchikova E, Powell R, Fukuyo Y, Nakamura T, Tran U, Oda S, Tanaka M, Wessely O, Kurihara H, Sakai T, Obara T
  • Journal Title: PLoS One Volume: 7(9) Issue: 9 Pages: e45286-e45286
  • DOI: 10.1371/journal.pone.0045286
  • Peer Reviewed
• [Journal Article] Wtip and Vangl2 are required for mitotic spindle orientation and cloaca morphogenesis (2012)
  • Author(s): Bubenshchikova E, Ichimura K, Fukuyo Y, Powell R, Hsu C, Morrical SO, Sedor JR, Sakai T, Obara T
  • Journal Title: Biology Open Volume: 1(6) Issue: 6 Pages: 588-596
  • DOI: 10.1242/bio.20121016
  • Peer Reviewed
• [Journal Article] 構造変化から見た腎臓の老化 (2012)
  • Author(s): 市村浩一郎、坂井建雄
  • Journal Title: 日本腎臓学会誌 Volume: 54 Pages: 59-62
• [Presentation] 多細胞動物における原尿産生装置の進化 (2014)
  • Author(s): 市村浩一郎
  • Organizer: 第105回東京腎生理集談会
  • Place of Presentation: 東京
  • Year and Date: 2014-05-24
  • Invited
• [Presentation] 多細胞動物における原尿産生装置の進化 (2014)
  • Author(s): 市村浩一郎
  • Organizer: 第55回信州腎セミナー
  • Place of Presentation: 松本市
  • Year and Date: 2014-03-08
  • Invited
• [Presentation] 多細胞動物における原尿産生装置の進化 (2013)
  • Author(s): 市村浩一郎
  • Organizer: 第7回蠕虫研究会
  • Place of Presentation: 藤沢市
  • Year and Date: 2013-11-15
  • Invited
• [Presentation] 哺乳類における腎糸球体の進化と老化 (2013)
  • Author(s): 市村浩一郎
  • Organizer: 第14回腎セミナー
  • Place of Presentation: 大津市
  • Year and Date: 2013-08-08
  • Invited
• [Presentation] 糸球体疾患における濾過障壁の構造変化 (2013)
  • Author(s): 市村浩一郎、坂井建雄
  • Organizer: 第118回日本解剖学会学術総会
  • Place of Presentation: 高松市 (シンポジウム)
  • Year and Date: 2013-03-28
• [Presentation] ゼブラフィッシュmpp5a/nagie oko変異体における前腎糸球体の形成異常 (2013)
  • Author(s): 市村浩一郎、福与弥生、中村友美、レベッカパウエル、坂井建雄、小原朋子
  • Organizer: 第118回日本解剖学会学術総会
  • Place of Presentation: 高松市
  • Year and Date: 2013-03-28
• [Presentation] 糸球体疾患における濾過障壁の構造変化 (2013)
  • Author(s): 市村浩一郎、坂井建雄
  • Organizer: 第118回日本解剖学会学術総会
  • Place of Presentation: 高松市
  • Invited
• [Presentation] Pronephric glomerulus development in zebrafish and medaka (2012)
  • Author(s): Ichimura K, Bubenshchikova E, Powell R, Fukuyo Y, Nakamura T, Tran U, Oda S, Tanaka M, Wessely O, Kurihara H, Sakai T, Obara T
  • Organizer: 10th International Conference Zebrafish Development and Genetics
  • Place of Presentation: Madison, Wisconsin, USA
  • Year and Date: 2012-06-20
• [Presentation] Using both medaka and zebrafish to study glomerulus development and slit diaphragms (2012)
  • Author(s): Ichimura K, Bubenshchikova E, Powell R, Fukuyo Y, Nakamura T, Tran U, Oda S, Tanaka M, Wessely O, Kurihara H, Sakai T, Obara T
  • Organizer: 9th Internaltional Podocyte Conference
  • Place of Presentation: Miami, Florida, USA
  • Year and Date: 2012-04-22
• [Presentation] 糸球体構成細胞における一次線毛の構造 (2011)
  • Author(s): 市村浩一郎、坂井建雄
  • Organizer: 第54回日本腎臓学会学術総会(招待講演)
  • Place of Presentation: パシフィコ横浜（横浜市）
• [Presentation] 哺乳類における腎糸球体の進化と老化
  • Author(s): 市村浩一郎
  • Organizer: 第14回腎セミナー
  • Place of Presentation: 滋賀医科大学（大津市）
  • Invited
• [Presentation] 多細胞動物における原尿産生装置の進化
  • Author(s): 市村浩一郎
  • Organizer: 第7回蠕虫研究会
  • Place of Presentation: KKR江の島ニュー向洋（藤沢市）
  • Invited
• [Presentation] 多細胞動物における原尿産生装置の進化
  • Author(s): 市村浩一郎
  • Organizer: 第55回信州腎セミナー
  • Place of Presentation: 護国神社美須々会館（松本市）
  • Invited
• [Presentation] 多細胞動物における原尿産生装置の進化
  • Author(s): 市村浩一郎
  • Organizer: 第105回東京腎生理集談会
  • Place of Presentation: ソラシティカンファレンスホール（東京）
  • Invited
• [Book] スンクスの生物学(第1版)腎臓、尿管、膀胱の構造 (2011)
  • Author(s): 市村浩一郎、寺山隼人(著)、磯村源藏、織田銑一、東家一雄、宮木孝昌(監修)
  • Publisher: 学会出版センター (東京)