| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
Using transmission electron microscope, we investigated anchoring system between myofibrils and sarcolemma by washing out cytoplasmic soluble proteins with various methods. Actin and intermediate filaments links between myofibrils and sarcolemma and many of them are anchored to the subsarcolemmal densities. These filamentous structures were less common in skeletal muscles of mdx mice. We also investigated the functions of synemin that is a component of intermediate filaments in skeletal muscle. Through experiments, it may relate to the endurance of skeletal muscle and the change of fiber types. Furthermore we investigated relationship of dystrophin and synemin. We found that deficiency of dystrophin and synemin affected fertility. During the development of Zebrafish embryo, it expressed in the heart from 56 to 72 hours after fertilization.
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