Analysis of the epithelial-mesenchymal transition (EMT) mechanism by utilizing neural crest cell generation model.

• Project/Area Number: 23590233
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Gifu University
• Principal Investigator: MOTOHASHI Tsutomu 岐阜大学, 医学(系)研究科(研究院), 講師 (40334932)
• Co-Investigator(Renkei-kenkyūsha): WAKAOKA Takanori 岐阜大学, 大学院医学系研究科, 大学院生 ; KITAGAWA Daisuke 岐阜大学, 大学院医学系研究科, 大学院生
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 上皮-間葉系転換 / 神経堤細胞 / 転写因子Sox10 / ES細胞 / 遺伝子改変マウス

Research Abstract

We identified the up-regulated genes in epithelial-mesenchymal transition (EMT) by utilizing the embryonic stem cells and mice, where the expression of Sox10 can be visualized with a fluorescent protein. Among these genes, we found the transcription factors that enhanced EMT. In addition, we found that glycoprotein Galectin-1 enhanced neural crest cell generation.

Research Products

• [Journal Article] Neural crest-derived cells sustain their multipotency even after entry into their target tissues (2014)
  • Author(s): Tsutomu Motohashi, Daisuke Kitagawa, Natsuki Watanabe, Takanori Wakaoka, Takahiro Kunisada
  • Journal Title: Developmental Dynamics Volume: 243 Pages: 368-380
  • Peer Reviewed
• [Journal Article] Neural crest-derived cells sustain their multipotency even after entry into their target tissues. (2014)
  • Author(s): Tsutomu Motohashi, Daisuke Kitagawa, Natsuki Watanabe, Takanori Wakaoka, Takahiro Kunisada
  • Journal Title: Developmental Dynamics Volume: 243 Issue: 3 Pages: 368-380
  • DOI: 10.1002/dvdy.24072
  • Peer Reviewed
• [Journal Article] Keratinocyte Stem cells but not Melanocyte Stem cells are the primary target for Radiation-induced Hair Graying (2013)
  • Author(s): Hitomi Aoki, Akira Hara, Tsutomu Motohashi, Takahiro Kunisada
  • Journal Title: Journal of Investigative Dermatology Volume: 133 Pages: 2143-2151
  • Peer Reviewed
• [Journal Article] Tracing Sox10-expressing cells elucidates the dynamic development of the mouse inner ear (2013)
  • Author(s): Takanori Wakaoka, Tsutomu Motohashi, Hisamitsu Hayashi, Mitsuhiro Aoki, Keisuke Mizuta, Takahiro Kunisada, Yatsuji Ito
  • Journal Title: Hearing Research Volume: 302 Pages: 17-25
  • Peer Reviewed
• [Journal Article] Dual origin of melanocytes defined by Sox 1 expression and their region-specific distribution in mammalian skin (2013)
  • Author(s): Naoko Yoshimura, Tsutomu Motohashi, Hitomi Aoki, Ken-ichi Tezuka, Natsuki Watanabe, Takanori Wakaoka, Takumi Era, Takahiro Kunisada
  • Journal Title: Development, Growth & Differentiation Volume: 55 Pages: 270-281
  • NAID: 40019734976
  • Peer Reviewed
• [Journal Article] Keratinocyte Stem cells but not Melanocyte Stem cells are the primary target for Radiation-induced Hair Graying. (2013)
  • Author(s): Hitomi Aoki, Akira Hara, Tsutomu Motohashi, Takahiro Kunisada
  • Journal Title: Journal of Investigative Dermatology. Volume: 133 Issue: 9 Pages: 2143-2151
  • DOI: 10.1038/jid.2013.155
  • Peer Reviewed
• [Journal Article] Tracing Sox10-expressing cells elucidates the dynamic development of the mouse inner ear. (2013)
  • Author(s): Takanori Wakaoka, Tsutomu Motohashi, Hisamitsu Hayashi, Mitsuhiro Aoki, Keisuke Mizuta, Takahiro Kunisada, Yatsuji Ito
  • Journal Title: Hearing Research Volume: 302 Pages: 17-25
  • DOI: 10.1016/j.heares.2013.05.003
  • Peer Reviewed
• [Journal Article] Dual origin of melanocytes defined by Sox 1 expression and their region-specific distribution in mammalian skin. (2013)
  • Author(s): Naoko Yoshimura, Tsutomu Motohashi, Hitomi Aoki, Ken-ichi Tezuka, Natsuki Watanabe, Takanori Wakaoka, Takumi Era, Takahiro Kunisada
  • Journal Title: Development, Growth and Differentiation Volume: 55 Issue: 2 Pages: 270-281
  • DOI: 10.1111/dgd.12034
  • NAID: 40019734976
  • Peer Reviewed
• [Journal Article] Neural crest cells retain their capability for multipotential differentiation even after lineage-restricted stages (2011)
  • Author(s): Tsutomu Motohashi, Katsumasa Yamanaka, Kairi Chiba, Kentaro Miyajima, Hitomi Aoki, Tomohisa Hirobe, Takahiro Kunisada
  • Journal Title: Developmental Dynamics Volume: 240 Pages: 1681-1693
  • Peer Reviewed
• [Journal Article] Neural crest cells retain their capability for multipotential differentiation even after lineage-restricted stages. (2011)
  • Author(s): Tsutomu Motohashi, Katsumasa Yamanaka, Kairi Chiba, Kentaro Miyajima, Hitomi Aoki, Tomohisa Hirobe, Takahiro Kunisada.
  • Journal Title: Developmental Dynamics. Volume: 240 Issue: 7 Pages: 1681-1693
  • DOI: 10.1002/dvdy.22658
  • Peer Reviewed
• [Presentation] Tracing Sox10-expressing cells elucidates the dynamic development of the mouse inner ear. (2014)
  • Author(s): Takanori Wakaoka, Tsutomu Motohashi, Hisamitsu Hayashi, Bunya Kuze, Mitsuhiro Aoki, Keisuke Mizuta, Takahiro Kunisada, Yatsuji Ito
  • Organizer: 37th The Association for Research in Otolaryngology
  • Place of Presentation: San Diego, USA
• [Presentation] Galectin-1 promote the development of Neural crest cells (2013)
  • Author(s): 西岡真弘、本橋力
  • Organizer: 日本分子生物学会第36回年会
  • Place of Presentation: 神戸
  • Year and Date: 2013-12-04
• [Presentation] Notch signal play a role in the multipotency of melanoblast (2013)
  • Author(s): 渡邉奈月、本橋力
  • Organizer: 第25回日本色素細胞学会学術大会
  • Place of Presentation: 大阪
  • Year and Date: 2013-11-16
• [Presentation] Neural Crest-Derived Cells sustain the multipotency even after intrusion into the tissues (2013)
  • Author(s): Tsutomu Motohashi
  • Organizer: 日本発生生物学会第46回大会
  • Place of Presentation: 松江
  • Year and Date: 2013-05-31
• [Presentation] Notch signal play a role in the multipotency of melanoblast (2013)
  • Author(s): Natsuki Watanabe, Tsutomu Motohashi
  • Organizer: 日本発生生物学会第46回大会
  • Place of Presentation: 松江
  • Year and Date: 2013-05-29
• [Presentation] 色素芽細胞のもつ多分化能におけるNotchシグナル (2013)
  • Author(s): 渡邊奈月、本橋力
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2013-03-21
• [Presentation] 色素芽細胞のもつ多分化能におけるNotchシグナル (2013)
  • Author(s): 渡邊奈月，北川大祐，西岡真弘，若岡敬紀，広部知久，本橋力，國貞隆弘
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: 横浜市
• [Presentation] 内耳幹細胞の分離及びその分化能の解析 (2012)
  • Author(s): 若岡敬紀、本橋力
  • Organizer: 第11回日本再生医療学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2012-06-12
• [Presentation] Novel genes of neural crest cells generation elucidated by gene expression analysis (2012)
  • Author(s): Tsutomu Motohashi
  • Organizer: 日本発生生物学会第45回大会
  • Place of Presentation: 神戸
  • Year and Date: 2012-05-30
• [Presentation] Sox10-IRES-GFPマウスを利用した内耳発生の解析 (2012)
  • Author(s): 若岡敬紀、本橋力
  • Organizer: 第113回日本耳鼻咽喉科学会総会
  • Place of Presentation: 新潟
• [Presentation] Sox10-IRES-GFPマウスを利用した内耳発生の解析 (2012)
  • Author(s): 若岡敬紀、本橋力、林寿光、久世文也、青木光広、水田啓介、伊藤八次
  • Organizer: 第113回日本耳鼻咽喉科学会総会
  • Place of Presentation: 新潟市
• [Presentation] Novel genes of neural crest cells generation elucidated by gene expression analysis. (2012)
  • Author(s): Tsutomu Motohashi, Daisuke Kitagawa, Takanori Wakaoka, Natsuki Watanabe, Takahiro Kunisada
  • Organizer: 45th Annual Meeting of the Japanese Society of Developmental Biologists
  • Place of Presentation: 神戸市
• [Presentation] 内耳幹細胞の分離及びその分化能の解析 (2012)
  • Author(s): 若岡敬紀、本橋力、林寿光、久世文也、青木光広、水田啓介、伊藤八次、國貞隆弘
  • Organizer: 第11回日本再生医療学会総会
  • Place of Presentation: 横浜市
• [Presentation] Gene expression analysis of Neural crest cells in their early developmental stage (2011)
  • Author(s): Daisuke Kitagawa, Tsutomu Motohashi
  • Organizer: 日本分子生物学会第34回年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] Gene expression analysis of Neural crest cells in their early developmental stage. (2011)
  • Author(s): Daisuke Kitagawa, Tsutomu Motohashi, Takanori Wakaoka, Natsuki Watanabe, Takahiro Kunisada
  • Organizer: 日本分子生物学会 第34回年会
  • Place of Presentation: 横浜
• [Presentation] Neural Crest-Derived Cells sustain the multipotency even after intrusion into the tissues.
  • Author(s): Tsutomu Motohashi , Natsuki Watanabe, Masahiro Nishioka, Takanori Wakaoka, Takahiro Kunisada
  • Organizer: 第46回日本発生生物学会
  • Place of Presentation: 松江市
• [Presentation] Notch signal play a role in the multipotency of melanoblast.
  • Author(s): 渡邉奈月、西岡真弘、若岡敬紀、広部知久、本橋 力、國貞隆弘
  • Organizer: 第46回日本発生生物学会
  • Place of Presentation: 松江市
• [Presentation] 色素芽細胞の持つ多分化能にNotchシグナルが重要である
  • Author(s): 渡邉奈月、西岡真弘、若岡敬紀、広部知久、本橋 力、國貞隆弘
  • Organizer: 第25回日本色素細胞学会学術大会
  • Place of Presentation: 吹田市
• [Presentation] Galectin-1 promote the development of Neural crest cells.
  • Author(s): 西岡 真弘, 本橋 力, 北川 大祐, 渡邉 奈月, 若岡 敬紀, 門屋 利彦, 國貞 隆弘
  • Organizer: 日本分子生物学会 第36回年会
  • Place of Presentation: 神戸市
• [Book] Skin Stem Cells Methods and Protocols (2013)
  • Author(s): Tsutomu Motohashi, Takahiro Kunisada
  • Publisher: Human Press
• [Remarks]
  • URL: http://www.med.gifu-u.ac.jp/saisei/index.htm